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We compared the performance of the KK method and real-time PCR in the framework of a randomized trial, which evaluated four novel treatments against <jats:italic>Trichuris trichiura<\/jats:italic> and concomitant STH infections.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>Two stool samples obtained from 320 participants were examined at baseline and follow-up with quadruplicate KK\u00a0and PCR analyses of one of the two samples using \u201cbead-beating\u201d for DNA extraction. At follow-up, 80 samples were negative according to both PCR and KK\u00a0and 173 were positive with both methods for any of the STHs. Relative to PCR, the calculated sensitivity of KK at follow-up was 83.6%, 43.0% and 53.8% for <jats:italic>T. trichiura<\/jats:italic>, for hookworm and for <jats:italic>Ascaris lumbricoides<\/jats:italic>, respectively. The sensitivity of PCR compared with KK at this time point was 89.1% for <jats:italic>T. trichiura<\/jats:italic>, 72.7% for hookworm and 87.5% for <jats:italic>A. lumbricoides.<\/jats:italic> Cure rates (CRs) for <jats:italic>T. trichiura<\/jats:italic> and <jats:italic>A. lumbricoides<\/jats:italic> were slightly lower with the PCR method. For hookworm CRs with KK were mostly significantly lower, namely 36.7%, 91.1%, 72.2% and 77.8% for moxidectin, moxidectin in combination with tribendimidine, moxidectin in combination with albendazole and albendazole in combination with oxantel pamoate, respectively, whereas with PCR the CRs were 8.3%, 82.6%, 37.1% and 57.1%, respectively.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>In conclusion, a single real-time PCR is as sensitive as quadruplicate KK\u00a0for <jats:italic>T. trichiura<\/jats:italic> and <jats:italic>A. lumbricoides<\/jats:italic> detection but more sensitive for hookworm<jats:italic>,<\/jats:italic> which has an influence on the estimated treatment efficacy. PCR method with DNA extraction using the \u201cbead-beating protocol\u201d should be further promoted in endemic areas and laboratories that can afford the needed equipment.<\/jats:p>\n<jats:p>The study is registered at ISRCTN (no. 20398469).<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12866-020-01963-9","type":"journal-article","created":{"date-parts":[[2020,10,2]],"date-time":"2020-10-02T09:03:22Z","timestamp":1601629402000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Comparison of real-time PCR and the Kato-Katz method for the diagnosis of soil-transmitted helminthiasis and assessment of cure in a randomized controlled trial"],"prefix":"10.1186","volume":"20","author":[{"given":"Beatrice","family":"Barda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Schindler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahel","family":"Wampfler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaali","family":"Ame","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Said M.","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0290-3521","authenticated-orcid":false,"given":"Jennifer","family":"Keiser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,10,2]]},"reference":[{"key":"1963_CR1","doi-asserted-by":"publisher","first-page":"1211","DOI":"10.1016\/S0140-6736(17)32154-2","volume":"390","author":"GBD 2016 Disease and Injury Incidence and Prevalence Collaborators","year":"2017","unstructured":"GBD 2016 Disease and Injury Incidence and Prevalence Collaborators. 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