{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T01:04:29Z","timestamp":1777511069515,"version":"3.51.4"},"reference-count":14,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,9,17]],"date-time":"2020-09-17T00:00:00Z","timestamp":1600300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2020,9,17]],"date-time":"2020-09-17T00:00:00Z","timestamp":1600300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100003977","name":"Israeli Science Foundation","doi-asserted-by":"crossref","award":["739\/19"],"award-info":[{"award-number":["739\/19"]}],"id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n<jats:title>Background<\/jats:title>\n<jats:p>Tolerance to antibiotics and persistence are associated with antibiotic treatment failures, chronic-relapsing infections, and emerging antibiotic resistance in various bacteria, including <jats:italic>Staphylococcus aureus<\/jats:italic>. Mechanisms of persistence are largely unknown, yet have been linked to physiology under low-ATP conditions and the metabolic-inactive state. EttA is an ATP-binding cassette protein, linked in <jats:italic>Eschrechia coli<\/jats:italic> to ribosomal hibernation and fitness in stationary growth phase, yet its role in <jats:italic>S. aureus<\/jats:italic> physiology is unknown.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>Using whole genome sequencing (WGS) of serial clinical isolates, we identified an EttA-negative <jats:italic>S. aureus<\/jats:italic> mutant (<jats:italic>ettA<\/jats:italic><jats:sup><jats:italic>stop<\/jats:italic><\/jats:sup>), and its isogenic wild-type counterpart. We used these two isogenic clones to investigate the role of <jats:italic>ettA<\/jats:italic> in <jats:italic>S. aureus<\/jats:italic> physiology in starvation and antibiotic stress, and test its role in persistence and antibiotic tolerance. <jats:italic>ettA<\/jats:italic><jats:sup><jats:italic>stop<\/jats:italic><\/jats:sup> and its WT counterpart were similar in their antibiotic resistance profiles to multiple antibiotics. Population dynamics of <jats:italic>ettA<\/jats:italic><jats:sup><jats:italic>stop<\/jats:italic><\/jats:sup> and the WT were similar in low-nutrient setting, with similar recovery from stationary growth phase or starvation. Supra-bacteriocidal concentration of cefazolin had the same killing effect on <jats:italic>ettA<\/jats:italic><jats:sup><jats:italic>stop<\/jats:italic><\/jats:sup> and WT populations, with no difference in persister formation.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>Lack of <jats:italic>ettA<\/jats:italic> does not affect <jats:italic>S. aureus<\/jats:italic> antibiotic resistance, beta-lactam tolerance, resilience to starvation or fitness following starvation. We conclude the role of <jats:italic>ettA<\/jats:italic> in <jats:italic>S. aureus<\/jats:italic> physiology is limited or redundant with another, unidentified gene. WGS of serial clinical isolates may enable investigation of other single genes involved in <jats:italic>S. aureus<\/jats:italic> virulence, and specifically persister cell formation.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12866-020-01970-w","type":"journal-article","created":{"date-parts":[[2020,9,17]],"date-time":"2020-09-17T15:15:30Z","timestamp":1600355730000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["EttA is likely non-essential in Staphylococcus aureus persistence, fitness or resistance to antibiotics"],"prefix":"10.1186","volume":"20","author":[{"given":"Michal","family":"Meir","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Rozenblit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simona","family":"Fliger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuval","family":"Geffen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Barkan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,9,17]]},"reference":[{"issue":"7346","key":"1970_CR1","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1038\/nature10069","volume":"473","author":"KR Allison","year":"2011","unstructured":"Allison KR, Brynildsen MP, Collins JJ. 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