{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T07:36:51Z","timestamp":1780472211513,"version":"3.54.1"},"reference-count":17,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,3,31]],"date-time":"2021-03-31T00:00:00Z","timestamp":1617148800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,3,31]],"date-time":"2021-03-31T00:00:00Z","timestamp":1617148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>16S rRNA gene sequencing is currently the most common way of determining the composition of microbiota. This technique has enabled many new discoveries to be made regarding the relevance of microbiota to the health and disease of the host. However, compared to other diagnostic techniques, 16S rRNA gene sequencing is fairly costly and labor intensive, leaving room for other techniques to improve on these aspects.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The current study aimed to compare the output of 16S rRNA gene sequencing to the output of the quick IS-pro analysis, using vaginal swab samples from 297 women of reproductive age. 16S rRNA gene sequencing and IS-pro analyses yielded very similar vaginal microbiome profiles, with a median Pearson\u2019s R<jats:sup>2<\/jats:sup> of 0.97, indicating a high level of similarity between both techniques.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>We conclude that the results of 16S rRNA gene sequencing and IS-pro are highly comparable and that both can be used to accurately determine the vaginal microbiota composition, with the IS-pro analysis having the benefit of rapidity.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-021-02149-7","type":"journal-article","created":{"date-parts":[[2021,3,31]],"date-time":"2021-03-31T17:03:02Z","timestamp":1617210182000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["The profiling of microbiota in vaginal swab samples using 16S rRNA gene sequencing and IS-pro analysis"],"prefix":"10.1186","volume":"21","author":[{"given":"M.","family":"Singer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Koedooder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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All the procedures and methods have been performed according to the guidelines of the ethics committee and in accordance with local laws and regulations. All participants were informed of the study contents and signed an informed consent form before inclusion into the study.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors would like to state a number of competing interests. The author AEB is co-founder of InBiome B.V. and together with author PHMS co-inventer of the IS-pro technology. The authors LP and MPB report that they are employees at Inbiome B.V., the authors RK and JDdJ report that they are an employee at ARTPred B.V.. JSEL reports consultancy fees from Titus Health Care. The Authors MS, SS and SAM declare that there is no conflict of interest.JDdJ and AEB have obtained patents \u2018Microbial population analysis\u2019 (9506109) and \u2018Microbial population analysis\u2019 (20170159108), both licenced to ARTPred B.V.. JDdJ and AEB report patent applications \u2018Method and kit for predicting the outcome of an assisted reproductive technology procedure\u2019 (392EPP0) and patent \u2018Method and kit for altering the outcome of an assisted reproductive technology procedure\u2019 by ARTPred.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"100"}}