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The production of <jats:italic>VacA<\/jats:italic> in natural circumstances is complex with heavy workload and low yield. Therefore, it is very important to obtain recombinant VacA protein which is stable and biologically active. This study therefore aims to explore the expression, purification and stable storage of <jats:italic>VacA<\/jats:italic> toxin of <jats:italic>H. pylori<\/jats:italic> in E.coli, and to provide experimental basis for further exploration of the role of VacA in <jats:italic>H. pylori<\/jats:italic> -induced inflammation of cancer.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>A 2502-bp fragment and <jats:italic>VacA<\/jats:italic> gene were identified. An 89.7-kDa VacA<jats:sup>34\u2013854<\/jats:sup> recombinant protein was expressed and purified from the recombinant engineering bacteria and was preserved stably in 50\u2009mM acetic acid buffer (pH\u20092.9). The amount of the recombinant protein was larger in the inclusion bodies than in the supernatant. In addition, after a 24-h culture with VacA recombinant protein, GES-1 cells demonstrated evidence of apoptosis including early nuclear immobilization and clustering under inverted microscope and TEM. It was found that VacA recombinant protein induced apoptosis by TUNEL assay.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>A VacA recombinant protein that is stably and highly expressed and possesses pro-apoptotic activity is successfully constructed. The protein is stably preserved in 50\u2009mM acetic acid buffer (pH\u20092.9).<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-021-02262-7","type":"journal-article","created":{"date-parts":[[2021,8,18]],"date-time":"2021-08-18T09:02:59Z","timestamp":1629277379000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Construction and preservation of a stable and highly expressed recombinant Helicobacter pylori vacuolating cytotoxin A with apoptotic activity"],"prefix":"10.1186","volume":"21","author":[{"given":"Ling-Zhi","family":"Yuan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao-Peng","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Ming","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1387-1126","authenticated-orcid":false,"given":"Fen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,8,18]]},"reference":[{"issue":"3","key":"2262_CR1","doi-asserted-by":"publisher","first-page":"280","DOI":"10.23736\/S1121-421X.18.02481-9","volume":"64","author":"GM Buz\u00e1s","year":"2018","unstructured":"Buz\u00e1s GM. 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