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In particular, phosphorylated ArcA, as a global transcription factor, binds to the promoter regions of its target genes to regulate the expression of aerobic and anaerobic metabolism genes. However, the function of ArcA in <jats:italic>Plesiomonas shigelloides<\/jats:italic> is unknown.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>In the present study, <jats:italic>P. shigelloides<\/jats:italic> was used as the research object. The differences in growth, motility, biofilm formation, and virulence between the WT strain and the \u0394<jats:italic>arcA<\/jats:italic> isogenic deletion mutant strain were compared. The data showed that the absence of <jats:italic>arcA<\/jats:italic> not only caused growth retardation of <jats:italic>P. shigelloides<\/jats:italic> in the log phase, but also greatly reduced the glucose utilization in M9 medium before the stationary phase. The motility of the \u0394<jats:italic>arcA<\/jats:italic> mutant strain was either greatly reduced when grown in swim agar, or basically lost when grown in swarm agar. The electrophoretic mobility shift assay results showed that ArcA bound to the promoter regions of the <jats:italic>flaK<\/jats:italic>, <jats:italic>rpoN<\/jats:italic>, and <jats:italic>cheV<\/jats:italic> genes, indicating that ArcA directly regulates the expression of these three motility-related genes in <jats:italic>P. shigelloides<\/jats:italic>. Meanwhile, the ability of the \u0394<jats:italic>arcA<\/jats:italic> strain to infect Caco-2 cells was reduced by 40%; on the contrary, its biofilm formation was enhanced. Furthermore, the complementation of the WT <jats:italic>arcA<\/jats:italic> gene from pBAD33-<jats:italic>arcA<\/jats:italic><jats:sup>+<\/jats:sup> was constructed and all of the above features of the pBAD33-<jats:italic>arcA<\/jats:italic><jats:sup>+<\/jats:sup> complemented strain were restored to the WT level.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>We showed the effect of ArcA on the growth, motility, biofilm formation, and virulence of <jats:italic>Plesiomonas shigelloides<\/jats:italic>, and demonstrated that ArcA functions as a positive regulator controls the motility of <jats:italic>P. shigelloides<\/jats:italic> by directly regulating the expression of <jats:italic>flaK<\/jats:italic>, <jats:italic>rpoN<\/jats:italic> and <jats:italic>cheV<\/jats:italic> genes.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-021-02322-y","type":"journal-article","created":{"date-parts":[[2021,10,4]],"date-time":"2021-10-04T17:35:57Z","timestamp":1633368957000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["The effect of ArcA on the growth, motility, biofilm formation, and virulence of Plesiomonas shigelloides"],"prefix":"10.1186","volume":"21","author":[{"given":"Junxiang","family":"Yan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuehua","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueqian","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaochen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fenxia","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boyang","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,10,4]]},"reference":[{"issue":"18","key":"2322_CR1","doi-asserted-by":"publisher","first-page":"6788","DOI":"10.3390\/ijms21186788","volume":"21","author":"A Maciejewska","year":"2020","unstructured":"Maciejewska A, Bednarczyk B, Lugowski C, Lukasiewicz J. 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