{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T10:27:20Z","timestamp":1784543240488,"version":"3.55.0"},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2022,1,8]],"date-time":"2022-01-08T00:00:00Z","timestamp":1641600000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,1,8]],"date-time":"2022-01-08T00:00:00Z","timestamp":1641600000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p><jats:italic>Legionella pneumophila<\/jats:italic> is an opportunistic waterborne pathogen of significant public health problems, which can cause serious human respiratory diseases (Legionnaires\u2019 disease). Multiple cross displacement amplification (MCDA), a isothermal nucleic acid amplification technique, has been applied in the rapid detection of several bacterial agents. In this report, we developed a MCDA coupled with Nanoparticles-based Lateral Flow Biosensor (MCDA-LFB) for the rapid detection of <jats:italic>L. pneumophila<\/jats:italic>.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>A set of 10 primers based on the <jats:italic>L. pneumophila<\/jats:italic> specific <jats:italic>mip<\/jats:italic> gene to specifically identify 10 different target sequence regions of <jats:italic>L. pneumophila<\/jats:italic> was designed. The optimal time and temperature for amplification are 57\u2009min and 65\u2009\u00b0C. The limit of detection (LoD) is 10\u2009fg in pure cultures of <jats:italic>L. pneumophila<\/jats:italic>. No cross-reaction was obtained and the specificity of MCDA-LFB assay was 100%. The whole process of the assay, including 20\u2009min of DNA preparation, 35\u2009min of <jats:italic>L. pneumophila<\/jats:italic>-MCDA reaction, and 2\u2009min of sensor strip reaction, took a total of 57\u2009min (less than 1\u2009h). Among 88 specimens for clinical evaluation, 5 (5.68%) samples were <jats:italic>L. pneumophila<\/jats:italic>-positive by MCDA-LFB and traditional culture method, while 4(4.55%) samples were <jats:italic>L. pneumophila<\/jats:italic>-positive by PCR method targeting <jats:italic>mip<\/jats:italic> gene. Compared with culture method, the diagnostic accuracy of MCDA-LFB method was higher.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>In summary, the <jats:italic>L. pneumophila<\/jats:italic>-MCDA-LFB method we successfully developed is a simple, fast, reliable and sensitive diagnostic tool, which can be widely used in basic and clinical laboratories.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-021-02363-3","type":"journal-article","created":{"date-parts":[[2022,1,8]],"date-time":"2022-01-08T03:02:47Z","timestamp":1641610967000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Multiple Cross Displacement Amplification Coupled with Lateral Flow Biosensor (MCDA-LFB) for rapid detection of Legionella pneumophila"],"prefix":"10.1186","volume":"22","author":[{"given":"Luxi","family":"Jiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rumeng","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomeng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meijun","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojun","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deguang","family":"Mu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,1,8]]},"reference":[{"issue":"2","key":"2363_CR1","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1128\/CMR.00052-09","volume":"23","author":"HJ Newton","year":"2010","unstructured":"Newton HJ, Ang DK, van Driel IR, Hartland EL. 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