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Within this pathway, the activation of the transcription factor Pdr3 induces transcription of the ATP-binding cassette (ABC) transporter gene, <jats:italic>PDR5,<\/jats:italic> and causes pleiotropic drug resistance (PDR). Although a histone deacetylase, Rpd3, is also required for cycloheximide resistance in \u03c1<jats:sup>0\/\u2212<\/jats:sup> cells, it is currently unknown whether Rpd3 and its DNA binding partners, Ume6 and Ash1, are involved in the activation of <jats:italic>PDR5<\/jats:italic> transcription and PDR in \u03c1<jats:sup>0\/\u2212<\/jats:sup> cells. This study investigated the roles of <jats:italic>RPD3<\/jats:italic>, <jats:italic>UME6<\/jats:italic>, and <jats:italic>ASH1<\/jats:italic> in the activation of <jats:italic>PDR5<\/jats:italic> transcription and PDR by retrograde signalling in \u03c1<jats:sup>0<\/jats:sup> cells.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>\u03c1<jats:sup>0<\/jats:sup> cells in the <jats:italic>rpd3<\/jats:italic>\u2206 and <jats:italic>ume6<\/jats:italic>\u2206 strains, with the exception of the <jats:italic>ash1<\/jats:italic>\u2206 strain, were sensitive to fluconazole and cycloheximide. The <jats:italic>PDR5<\/jats:italic> mRNA levels in \u03c1<jats:sup>0<\/jats:sup> cells of the <jats:italic>rpd3<\/jats:italic>\u2206 and <jats:italic>ume6<\/jats:italic>\u2206 strains were significantly reduced compared to the wild-type and <jats:italic>ash1<\/jats:italic>\u2206 strain. Transcriptional expression of <jats:italic>PDR5<\/jats:italic> was reduced in cycloheximide-exposed and unexposed \u03c1<jats:sup>0<\/jats:sup> cells of the <jats:italic>ume6<\/jats:italic>\u2206 strain; the transcriptional positive response of <jats:italic>PDR5<\/jats:italic> to cycloheximide exposure was also impaired in this strain.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p><jats:italic>RPD3<\/jats:italic> and <jats:italic>UME6<\/jats:italic> are responsible for enhanced <jats:italic>PDR5<\/jats:italic> mRNA levels and PDR by retrograde signalling in \u03c1<jats:sup>0<\/jats:sup> cells of <jats:italic>S. cerevisiae<\/jats:italic>.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-021-02373-1","type":"journal-article","created":{"date-parts":[[2021,11,9]],"date-time":"2021-11-09T07:02:47Z","timestamp":1636441367000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["RPD3 and UME6 are involved in the activation of PDR5 transcription and pleiotropic drug resistance in \u03c10 cells of Saccharomyces cerevisiae"],"prefix":"10.1186","volume":"21","author":[{"given":"Yoichi","family":"Yamada","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,11,9]]},"reference":[{"key":"2373_CR1","doi-asserted-by":"publisher","first-page":"2206","DOI":"10.1016\/S0021-9258(17)42155-7","volume":"269","author":"E Balzi","year":"1994","unstructured":"Balzi E, Wang M, Leterme S, Dyck LV, Goffeau A. 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