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Hypervirulent <jats:italic>Klebsiella pneumoniae<\/jats:italic> (hvKP) has become a new threat to human health. In our study, we aimed to investigate the epidemiological characteristics of hvKP in population with ASB.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>A total of 61 <jats:italic>K. pneumoniae<\/jats:italic> isolates were collected from 7530 urine samples between October and December 2020. The strains were sensitive to most of the antimicrobial agents tested, but a polymyxin resistant strain was found (MIC&gt;16 \u03bcg\/mL). Three serotypes were detected, including K1 (16.4%, 10\/61), K5 (1.6%, 1\/61) and K57 (3.2%, 2\/61). Four strains (KPNY9, KPNY31, KPNY40, and KPNY42) carried a combination of two or more hypervirulent markers (<jats:italic>peg-344<\/jats:italic>, <jats:italic>iroB<\/jats:italic>, <jats:italic>iucA<\/jats:italic>, <jats:sub><jats:italic>p<\/jats:italic><\/jats:sub><jats:italic>rmpA<\/jats:italic>, and <jats:sub><jats:italic>p<\/jats:italic><\/jats:sub><jats:italic>rmpA2<\/jats:italic>), and their survival rates after <jats:italic>Galleria mellonella<\/jats:italic> infection were lower than those of the other strains (40.0 <jats:italic>vs<\/jats:italic>. 70.0%), suggesting that they were hvKP. These hvKP strains with lower biofilm forming ability than classical <jats:italic>K. pneumoniae<\/jats:italic> (0.2625 \u00b1 0.0579 <jats:italic>vs<\/jats:italic>. 0.6686 \u00b1 0.0661, <jats:italic>P<\/jats:italic> = 0.033) were identified as belonging to K2-ST65, K2-ST86, K57-ST592, and K2-ST5559 (a new ST type). KPNY31 (ST5559) shared a close genetic relationship with KPNY42 (ST86) and other ST86 isolates, which have been detected in both nosocomial and community-acquired infections.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The hvKP with relatively weak biofilm formation was detected in a population with ASB, which was more likely to cause bacteremia and serious consequences. A novel sequence type (ST5559) hvKP derived from ST86 was found. Therefore, hvKP should be monitored in the population with ASB.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-021-02413-w","type":"journal-article","created":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T09:22:59Z","timestamp":1642497779000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Distribution, characterization, and antibiotic resistance of hypervirulent Klebsiella pneumoniae isolates in a Chinese population with asymptomatic bacteriuria"],"prefix":"10.1186","volume":"22","author":[{"given":"Jun","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanbing","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengli","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengjun","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changhang","family":"Min","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongmei","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haichen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxiang","family":"Zou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,1,18]]},"reference":[{"key":"2413_CR1","doi-asserted-by":"publisher","first-page":"2669","DOI":"10.1007\/s00345-019-03071-4","volume":"38","author":"R \u00d6zt\u00fcrk","year":"2020","unstructured":"\u00d6zt\u00fcrk R, Murt A. 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This study was approved by the Central South University Ethics Committee (Changsha, Hunan Province, China) with ID 201703302. All participants provided written informed consent.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"29"}}