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The development of antimicrobial resistance is associated with multiple factors including mutations in the specific genes. To know the current status of mutation-mediated fluoroquinolone-resistance among <jats:italic>Salmonella enterica<\/jats:italic> serovars; Typhi, Paratyphi A, B and C, this study was focused on detecting <jats:italic>gyrA<\/jats:italic> ser83 mutation by restriction digestion analysis of <jats:italic>gyrA<\/jats:italic> gene using <jats:italic>HinfI<\/jats:italic> endonuclease.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>A total of 948 blood samples were processed for isolation of <jats:italic>Salmonella<\/jats:italic> spp. and 3.4% of them were found to be positive for <jats:italic>Salmonella<\/jats:italic> growth. Out of the 32 <jats:italic>Salmonella<\/jats:italic> isolates, 2.2% were <jats:italic>S. <\/jats:italic>Typhi\u00a0and 1.2% were <jats:italic>S. <\/jats:italic>Paratyphi\u00a0A. More interestingly, we observed less than 5% of isolates were resistant to first-line drugs including chloramphenicol, cotrimoxazole and ampicillin. More than 80% of isolates were resistant to fluoroquinolones accounting for 84.4% to levofloxacin followed by 87.5% to ofloxacin and 100% to ciprofloxacin by disc diffusion methods. However, the minimum inhibitory concentration method using agar dilution showed only 50% of isolates were resistant to ciprofloxacin. A total of 3.1% of isolates were multidrug-resistant. Similarly, 90.6% of the <jats:italic>Salmonella<\/jats:italic> isolates showed <jats:italic>gyrA<\/jats:italic> ser83 mutation with resistance to nalidixic acid.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The increased resistance to fluoroquinolones and nalidixic acid in <jats:italic>Salmonella<\/jats:italic> isolates in our study suggests the use of alternative drugs as empirical treatment. Rather, the treatment should focus on prescribing first-line antibiotics since we observed less than 5% of <jats:italic>Salmonella<\/jats:italic> isolates were resistant to these drugs.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-022-02456-7","type":"journal-article","created":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T05:04:28Z","timestamp":1644469468000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["gyrA ser83 mutation among fluoroquinolone-resistant Salmonella enterica serovars from enteric fever patients in tertiary care hospital, Kathmandu"],"prefix":"10.1186","volume":"22","author":[{"given":"Prashanna","family":"Adhikari","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roshani","family":"Maharjan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Subash","family":"Paudel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bikram","family":"Malla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pradeep Kumar","family":"Shah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anup","family":"Bastola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Upendra Thapa","family":"Shrestha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,2,10]]},"reference":[{"issue":"9973","key":"2456_CR1","doi-asserted-by":"publisher","first-page":"1136","DOI":"10.1016\/S0140-6736(13)62708-7","volume":"385","author":"J Wain","year":"2015","unstructured":"Wain J, Hendriksen RS, Mikoleit ML, Keddy KH, Ochiai RL. 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A copy of the information sheet and consent form was given to participants to obtain written consent before enrollment in the research and collecting samples. A local language-translated information sheet was read for illiterate participants. Informed consent was obtained from all the participants and in the case of children under 16\u2009years, both written informed consent with assent was obtained from a parent or guardian attending the hospital along with the participant. All the methods were carried out in accordance with the principles stated in the Declaration of Helsinki.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"We declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"51"}}