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Various antigenic proteins of<jats:italic>Plasmodium<\/jats:italic>are considered as the major targets for the development of an effective vaccine. The aim of the current study was a comprehensive analysis of the experimentally validated epitopes of<jats:italic>Plasmodium<\/jats:italic>obtained from various immunoassays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic>Plasmodium<\/jats:italic>species epitopes were prefetched from Immune Epitope Database (IEDB). Species specific classification of available epitopes was done for both human and murine malaria parasites. Further, these T cell and B cell epitopes along with MHC I\/II binders of different<jats:italic>Plasmodium<\/jats:italic>species were examined to find out their capability to induce IFN-\u03b3 and IL-10 using IFNepitope and IL-10 Pred, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The species-specific classification of 6874 unique epitopes resulted in the selection of predominant human and murine<jats:italic>Plasmodium<\/jats:italic>species. Further, the attempt was made to analyse the immune reactivity of these epitopes for their ability to induce cytokines namely IFN-\u03b3 and IL-10. Total, 2775 epitopes were predicted to possess IFN-\u03b3 inducing ability, whereas 1275 epitopes were found to be involved in the induction of IL-10.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study facilitates the assessment of<jats:italic>Plasmodium<\/jats:italic>epitopes and associated proteins as a potential approach to design and develop an epitope-based vaccine. Moreover, the results highlight the epitope-based immunization in malaria to induce a protective immune response.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12866-022-02480-7","type":"journal-article","created":{"date-parts":[[2022,3,11]],"date-time":"2022-03-11T07:03:02Z","timestamp":1646982182000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A comprehensive study of epitopes and immune reactivity among Plasmodium species"],"prefix":"10.1186","volume":"22","author":[{"given":"Meenu","family":"Kalkal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Kalkal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandeep Kumar","family":"Dhanda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veena","family":"Pande","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyoti","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,3,11]]},"reference":[{"key":"2480_CR1","doi-asserted-by":"publisher","DOI":"10.30875\/6c551ba0-en","volume-title":"World malaria report 2021","author":"Organization WH","year":"2021","unstructured":"Organization WH. 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