{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T06:15:35Z","timestamp":1776838535974,"version":"3.51.2"},"reference-count":16,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2022,3,10]],"date-time":"2022-03-10T00:00:00Z","timestamp":1646870400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,3,10]],"date-time":"2022-03-10T00:00:00Z","timestamp":1646870400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"the Science and Technology Innovation Project of Medical Health in Sanya","award":["2019YW20"],"award-info":[{"award-number":["2019YW20"]}]},{"name":"the Key Research and Development Program of Hainan Province","award":["ZDYF2019149"],"award-info":[{"award-number":["ZDYF2019149"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p><jats:italic>Burkholderia pseudomallei<\/jats:italic> (<jats:italic>B. pseudomallei<\/jats:italic>), as a highly pathogenic organism, causes melioidosis, which is a disease of public health importance in many tropical developing countries. Here, we present and validate a novel detection technique, termed multiple cross displacement amplification combined with nanoparticles-based lateral flow biosensor (MCDA-NB), for identifying <jats:italic>B. pseudomallei<\/jats:italic> and diagnosing melioidosis.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p><jats:italic>B. pseudomallei<\/jats:italic>-MCDA targets the <jats:italic>TTS1<\/jats:italic> (Type III secretion system gene cluster 1) to specifically design ten MCDA primers. The nanoparticles-based biosensor (NB) can be combined with <jats:italic>B. pseudomallei<\/jats:italic>-MCDA for visually, objective, simply and rapidly reporting reaction results. The optimal amplification conditions of <jats:italic>B. pseudomallei<\/jats:italic>-MCDA were 66\u00a0\u00b0C for 30\u00a0min. Assay\u2019s sensitivity was 100\u00a0fg of genomic DNA in the pure cultures, and the analytical specificity was 100% by the examination of 257 strains, including 228 <jats:italic>B. pseudomallei<\/jats:italic> and 29 non-<jats:italic>B. pseudomallei<\/jats:italic>. As a result, the whole detection procedure was completed within 50\u00a0min, including 15\u00a0min for genomic DNA preparation, 30\u00a0min for l MCDA reaction, and 2\u00a0min for the interpretation of the results visually by biosensor.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p><jats:italic>B. pseudomallei<\/jats:italic>-MCDA assay is a rapid, sensitive and specific method for the detection of <jats:italic>B. pseudomallei<\/jats:italic>, and can be used as a potential tool for melioidosis diagnose in basic, field and clinical laboratories.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-022-02485-2","type":"journal-article","created":{"date-parts":[[2022,3,10]],"date-time":"2022-03-10T06:02:47Z","timestamp":1646892167000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Reliable detection of Burkholderia pseudomallei using multiple cross displacement amplification label-based biosensor"],"prefix":"10.1186","volume":"22","author":[{"given":"Xiaoxia","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Licheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huaxiong","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chongzhen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiong","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,3,10]]},"reference":[{"issue":"3","key":"2485_CR1","doi-asserted-by":"publisher","first-page":"814","DOI":"10.1128\/JCM.01702-10","volume":"49","author":"C-C Ho","year":"2011","unstructured":"Ho C-C, Lau CC, Martelli P, Chan S-Y, Cindy W, et al. Novel pan-genomic analysis approach in target selection for multiplex PCR identification and detection of Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia cepacia complex species: a proof-of-concept study. J Clin Microbiol. 2011;49(3):814\u201321.","journal-title":"J Clin Microbiol"},{"issue":"1","key":"2485_CR2","doi-asserted-by":"publisher","first-page":"15008","DOI":"10.1038\/nmicrobiol.2015.8","volume":"1","author":"D Limmathurotsakul","year":"2016","unstructured":"Limmathurotsakul D, Golding N, Dance DA, Messina JP, Pigott DM, et al. Predicted global distribution of Burkholderia pseudomallei and burden of melioidosis. Nat Microbiol. 2016;1(1):15008.","journal-title":"Nat Microbiol"},{"issue":"2","key":"2485_CR3","doi-asserted-by":"publisher","first-page":"265","DOI":"10.3201\/eid2002.121891","volume":"20","author":"D Limmathurotsakul","year":"2014","unstructured":"Limmathurotsakul D, Wongsuvan G, Aanensen D, Ngamwilai S, Saiprom N, et al. Melioidosis caused by Burkholderia pseudomallei in drinking water, Thailand, 2012. Emerg Infect Dis. 2014;20(2):265.","journal-title":"Emerg Infect Dis"},{"issue":"7","key":"2485_CR4","doi-asserted-by":"publisher","first-page":"1286","DOI":"10.3201\/eid1707.100661","volume":"17","author":"T Stewart","year":"2011","unstructured":"Stewart T, Engelthaler DM, Blaney DD, Tuanyok A, Wangsness E, et al. Epidemiology and investigation of melioidosis, Southern Arizona. Emerg Infect Dis. 2011;17(7):1286.","journal-title":"Emerg Infect Dis"},{"issue":"1","key":"2485_CR5","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1128\/JCM.44.1.85-90.2006","volume":"44","author":"RT Novak","year":"2006","unstructured":"Novak RT, Glass MB, Gee JE, Gal D, Mayo MJ, et al. Development and evaluation of a real-time PCR assay targeting the type III secretion system of Burkholderia pseudomallei. 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Nano Res. 2018;11(5):2632\u201347.","journal-title":"Nano Res"},{"key":"2485_CR8","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.aca.2014.09.037","volume":"853","author":"H Deng","year":"2015","unstructured":"Deng H, Gao Z. Bioanalytical applications of isothermal nucleic acid amplification techniques. Anal Chim Acta. 2015;853:30\u201345.","journal-title":"Anal Chim Acta"},{"issue":"2","key":"2485_CR9","doi-asserted-by":"publisher","first-page":"568","DOI":"10.1128\/JCM.01817-07","volume":"46","author":"N Chantratita","year":"2008","unstructured":"Chantratita N, Meumann E, Thanwisai A, Limmathurotsakul D, Wuthiekanun V, et al. Loop-mediated isothermal amplification method targeting the TTS1 gene cluster for detection of Burkholderia pseudomallei and diagnosis of melioidosis. J Clin Microbiol. 2008;46(2):568\u201373.","journal-title":"J Clin Microbiol"},{"key":"2485_CR10","doi-asserted-by":"publisher","first-page":"11902","DOI":"10.1038\/srep11902","volume":"5","author":"Y Wang","year":"2015","unstructured":"Wang Y, Wang Y, Ma AJ, Li DX, Luo LJ, et al. Rapid and Sensitive Isothermal Detection of Nucleic-acid Sequence by Multiple Cross Displacement Amplification. Sci Rep. 2015;5:11902.","journal-title":"Sci Rep"},{"key":"2485_CR11","unstructured":"Wang Y, Wang Y, Wang H, Xu J, Ye C. A label-free technique for accurate detection of nucleic acid\u2013based self-avoiding molecular recognition systems supplemented multiple cross-displacement amplification and nanoparticles based biosensor. Artif Cells Nanomed Biotechnol. 2018;46(8):1671-84."},{"issue":"11","key":"2485_CR12","doi-asserted-by":"publisher","first-page":"1035","DOI":"10.1056\/NEJMra1204699","volume":"367","author":"WJ Wiersinga","year":"2012","unstructured":"Wiersinga WJ, Currie BJ, Peacock SJ. Melioidosis. New Engl J Med. 2012;367(11):1035\u201344.","journal-title":"New Engl J Med"},{"issue":"12","key":"2485_CR13","doi-asserted-by":"publisher","first-page":"e2","DOI":"10.3201\/eid1812.120638","volume":"18","author":"R Lipsitz","year":"2012","unstructured":"Lipsitz R, Garges S, Aurigemma R, Baccam P, Blaney DD, et al. Workshop on treatment of and postexposure prophylaxis for Burkholderia pseudomallei and B. mallei infection, 2010. Emerg Infect Dis. 2012;18(12):e2.","journal-title":"Emerg Infect Dis"},{"issue":"5","key":"2485_CR14","doi-asserted-by":"publisher","first-page":"374","DOI":"10.1099\/0022-1317-51-5-374","volume":"51","author":"L Rainbow","year":"2002","unstructured":"Rainbow L, Hart CA, Winstanley C. Distribution of type III secretion gene clusters in Burkholderia pseudomallei, B thailandensis and B mallei. 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Informed consent was obtained from all the patients prior to testing. All data\/isolates were analyzed anonymously.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"72"}}