{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:28:15Z","timestamp":1787020095582,"version":"build-2736575974"},"reference-count":63,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2022,5,24]],"date-time":"2022-05-24T00:00:00Z","timestamp":1653350400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,5,24]],"date-time":"2022-05-24T00:00:00Z","timestamp":1653350400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Bacterial pectinase is an enzyme that could be employed in numerous sectors to break down pectin polysaccharide compounds. The goal of this study is to find pectinase-producing bacteria in avocado peel waste and see if the pectinase enzyme produced can be used to make fruit juice clarification.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The researchers isolated four different bacterial strains from avocado peel waste samples. The potential two bacterial isolates that were identified as being\n                      <jats:italic>Serratia marcescens<\/jats:italic>\n                      and\n                      <jats:italic>Lysinibacillus macrolides.<\/jats:italic>\n                      Finally, the analysis of pectinase production and its application in fruit juice clarification were performed using one of the bacterial strains of\n                      <jats:italic>Serratia marcescens<\/jats:italic>\n                      . The clear apple, lemon, and mango juices were further processed to assess each juice's properties. The highest antioxidant activity was recorded in lemon juice samples. The lemon juice showed the highest total titratable acidity and total phenol content. Apple juices contained the highest total soluble solids, reducing sugar content, and viscosity and the mango juices have the maximum pH value recorded.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The pectinase isolated from the bacterium\u00a0\n                      <jats:italic>Serratia marcescens<\/jats:italic>\n                      \u00a0could clear fruit juices. This pectinase needs to be studied more to make sure it works better in the fruit industry and other businesses.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12866-022-02536-8","type":"journal-article","created":{"date-parts":[[2022,5,24]],"date-time":"2022-05-24T02:05:11Z","timestamp":1653357911000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Isolation and characterization of pectinase-producing bacteria (Serratia marcescens) from avocado peel waste for juice clarification"],"prefix":"10.1186","volume":"22","author":[{"given":"Setegn","family":"Haile","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandran","family":"Masi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mesfin","family":"Tafesse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,5,24]]},"reference":[{"issue":"1","key":"2536_CR1","doi-asserted-by":"publisher","first-page":"514","DOI":"10.20546\/ijcmas.2016.501.051","volume":"5","author":"GA Aaisha","year":"2016","unstructured":"Aaisha GA, Barate DL. Isolation and identification of pectinolytic bacteria from soil samples of Akola region, India. Int J Curr Microbiol Appl Sci. 2016;5(1):514\u201321.","journal-title":"Int J Curr Microbiol Appl Sci"},{"issue":"1","key":"2536_CR2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13213-021-01631-x","volume":"71","author":"C Masi","year":"2021","unstructured":"Masi C, Gemechu G, Tafesse M. Isolation, screening, characterization, and identification of alkaline protease-producing bacteria from leather industry effluent. Annals of Microbiology. 2021;71(1):1\u20131. https:\/\/doi.org\/10.1186\/s13213-021-01631-x.","journal-title":"Annals of Microbiology"},{"issue":"59","key":"2536_CR3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1042\/bse0590001","volume":"15","author":"PK Robinson","year":"2015","unstructured":"Robinson PK. Enzymes: principles and biotechnological applications. Essays Biochem. 2015;15(59):1. https:\/\/doi.org\/10.1042\/bse0590001.","journal-title":"Essays Biochem"},{"issue":"4","key":"2536_CR4","doi-asserted-by":"publisher","first-page":"705","DOI":"10.1007\/s12223-020-00776-7","volume":"65","author":"R Abdollahzadeh","year":"2020","unstructured":"Abdollahzadeh R, Pazhang M, Najavand S, Fallahzadeh-Mamaghani V, Amani-Ghadim AR. Screening of pectinase-producing bacteria from farmlands and optimization of enzyme production from selected strain by RSM. Folia Microbiologica. 2020;65(4):705\u201319.","journal-title":"Folia Microbiologica"},{"issue":"1","key":"2536_CR5","doi-asserted-by":"publisher","first-page":"13","DOI":"10.6026\/97320630010013","volume":"10","author":"V Ahmad","year":"2014","unstructured":"Ahmad V, Kamal A, Ahmad K, Khan MS. Protease characteristics of bacteriocin producing Lysinibacillus, isolated from fruits and vegetable waste. Bioinformation. 2014;10(1):13.","journal-title":"Bioinformation"},{"issue":"2","key":"2536_CR6","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1007\/s12600-011-0148-6","volume":"39","author":"MH Abd-Alla","year":"2011","unstructured":"Abd-Alla MH, Bashandy SR, Schnell S, Ratering S. Isolation and characterization of Serratia rubidaea from dark brown spots of tomato fruits. Phytoparasitica. 2011;39(2):175\u201383.","journal-title":"Phytoparasitica."},{"issue":"11","key":"2536_CR7","first-page":"632","volume":"14","author":"G Gemechu","year":"2020","unstructured":"Gemechu G, Masi C, Tafesse M, Kebede G. A review on the bacterial alkaline proteases. J Xidian Univ. 2020;14(11):632\u20134.","journal-title":"J Xidian Univ."},{"issue":"6","key":"2536_CR8","doi-asserted-by":"publisher","first-page":"238","DOI":"10.3390\/catal8060238","volume":"8","author":"J Chapman","year":"2018","unstructured":"Chapman J, Ismail AE, Dinu CZ. Industrial applications of enzymes: Recent advances, techniques, and outlooks. Catalysts. 2018 Jun;8(6):238. https:\/\/doi.org\/10.3390\/catal8060238.","journal-title":"Catalysts"},{"issue":"3","key":"2536_CR9","doi-asserted-by":"publisher","first-page":"1680","DOI":"10.3390\/ijms23031680","volume":"23","author":"E Wilmowicz","year":"2022","unstructured":"Wilmowicz E, Ku\u0107ko A, Alch\u00e9 JD, Czeszewska-Rosiak G, Florkiewicz AB, Kapusta M, Karwaszewski J. Remodeling of Cell Wall Components in Root Nodules and Flower Abscission Zone under Drought in Yellow Lupine. Int J Mol Sci. 2022;23(3):1680. https:\/\/doi.org\/10.3390\/ijms23031680.","journal-title":"Int J Mol Sci"},{"key":"2536_CR10","doi-asserted-by":"publisher","unstructured":"Nighojkar A, Patidar MK, Nighojkar S. Pectinases: production and applications for fruit juice beverages. Processing and sustainability of beverages. Woodhead Publishing; 2019;2:235\u201373. https:\/\/doi.org\/10.1016\/B978-0-12-815259-1.00008-2.\u00a0","DOI":"10.1016\/B978-0-12-815259-1.00008-2"},{"key":"2536_CR11","doi-asserted-by":"publisher","first-page":"2018","DOI":"10.1155\/2018\/2961767","volume":"3","author":"OJ Oumer","year":"2018","unstructured":"Oumer OJ, Abate D. Screening and molecular identification of pectinase producing microbes from coffee pulp. Biomed Res Int. 2018;3:2018. https:\/\/doi.org\/10.1155\/2018\/2961767.","journal-title":"Biomed Res Int"},{"key":"2536_CR12","doi-asserted-by":"publisher","unstructured":"Haile S, Ayele A. Pectinase from Microorganisms and Its Industrial Applications. The Scientific World Journal. 2022; 2022.https:\/\/doi.org\/10.1155\/2022\/1881305","DOI":"10.1155\/2022\/1881305"},{"issue":"1","key":"2536_CR13","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13213-021-01662-4","volume":"72","author":"G Korsa","year":"2022","unstructured":"Korsa G, Masi C, Konwarh R, Tafesse M. Harnessing the potential use of cellulolytic Klebsiella oxytoca (M21WG) and Klebsiella sp. (Z6WG) isolated from the guts of termites (Isoptera). Ann Microbiol. 2022;72(1):1\u20135. https:\/\/doi.org\/10.1186\/s13213-021-01662-4.","journal-title":"Ann Microbiol."},{"issue":"109","key":"2536_CR14","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.fbp.2018.02.005","volume":"1","author":"RL de Oliveira","year":"2018","unstructured":"de Oliveira RL, Dias JL, da Silva OS, Porto TS. Immobilization of pectinase from Aspergillus aculeatus in alginate beads and clarification of apple and umbu juices in a packed bed reactor. Food Bioprod Process. 2018 May;1(109):9\u201318.","journal-title":"Food Bioprod Process"},{"key":"2536_CR15","first-page":"1","volume":"1","author":"R Javed","year":"2018","unstructured":"Javed R, Nawaz A, Munir M, Hanif M, Mukhtar H, Haq IU, Abdullah R. Extraction, purification and industrial applications of pectinase: A review. J Biotechnol Biores. 2018;1:1\u20136.","journal-title":"J Biotechnol Biores."},{"issue":"6","key":"2536_CR16","doi-asserted-by":"publisher","first-page":"396","DOI":"10.1038\/sj.jim.7000010","volume":"24","author":"QK Beg","year":"2000","unstructured":"Beg QK, Bhushan B, Kapoor M, Hoondal GS. Production and characterization of thermostable xylanase and pectinase from Streptomyces sp. QG-11\u20133. J Ind Microbiol Biotechnol. 2000;24(6):396\u2013402.","journal-title":"J Ind Microbiol Biotechnol"},{"key":"2536_CR17","doi-asserted-by":"publisher","unstructured":"Raveendran S, Parameswaran B, Ummalyma SB, Abraham A, Mathew AK, Madhavan A, Rebello S, Pandey A. Applications of microbial enzymes in food industry. Food Technol Biotechnol. 2018;56(1):16. https:\/\/doi.org\/10.17113\/ftb.56.01.18.5491","DOI":"10.17113\/ftb.56.01.18.5491"},{"issue":"3","key":"2536_CR18","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/S0960-8524(00)00118-8","volume":"77","author":"DR Kashyap","year":"2001","unstructured":"Kashyap DR, Vohra PK, Chopra S, Tewari R. Applications of pectinases in the commercial sector: a review. Biores Technol. 2001;77(3):215\u201327. https:\/\/doi.org\/10.1016\/S0960-8524(00)00118-8.","journal-title":"Biores Technol"},{"issue":"2","key":"2536_CR19","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1080\/08905436.2012.670830","volume":"26","author":"YS Kumar","year":"2012","unstructured":"Kumar YS, Kumar PV, Reddy OV. Pectinase production from mango peel using Aspergillus foetidus and its application in processing of mango juice. Food Biotechnol. 2012;26(2):107\u201323. https:\/\/doi.org\/10.1080\/08905436.2012.670830.","journal-title":"Food Biotechnol"},{"issue":"2","key":"2536_CR20","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1016\/j.jfoodeng.2005.10.024","volume":"78","author":"P Rai","year":"2007","unstructured":"Rai P, Majumdar GC, Gupta SD, De S. Effect of various pretreatment methods on permeate flux and quality during ultrafiltration of mosambi juice. J food Eng. 2007;78(2):561\u20138.","journal-title":"J food Eng."},{"issue":"5","key":"2536_CR21","doi-asserted-by":"publisher","first-page":"173","DOI":"10.12988\/asb.2013.313","volume":"5","author":"I Darah","year":"2013","unstructured":"Darah I, Nisha M, Lim SH. Enhancement of polygalacturonase production from Enterobacter aerogenes NBO2 by submerged fermentation. Adv Stud Biol. 2013;5(5):173\u201389.","journal-title":"Adv Stud Biol."},{"issue":"1","key":"2536_CR22","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/j.jfoodeng.2006.10.013","volume":"81","author":"AL Abdullah","year":"2007","unstructured":"Abdullah AL, Sulaiman NM, Aroua MK, Noor MM. Response surface optimization of conditions for clarification of carambola fruit juice using a commercial enzyme. J Food Eng. 2007;81(1):65\u201371.","journal-title":"J Food Eng"},{"issue":"1","key":"2536_CR23","first-page":"956","volume":"9","author":"C Masi","year":"2020","unstructured":"Masi C. Immobilization of Pseudomonas Aeruginosa in different matrices for the production of alkaline protease. Int J Recent Technol Eng. 2020;9(1):956\u20139.","journal-title":"Int J Recent Technol Eng"},{"issue":"4","key":"2536_CR24","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/S0260-8774(99)00124-7","volume":"42","author":"F Vaillant","year":"1999","unstructured":"Vaillant F, Millan P, O\u2019Brien G, Dornier M, Decloux M, Reynes M. Crossflow microfiltration of passion fruit juice after partial enzymatic liquefaction. J Food Eng. 1999;42(4):215\u201324.","journal-title":"J Food Eng."},{"issue":"100","key":"2536_CR25","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1016\/j.wasman.2019.09.013","volume":"1","author":"M Kazemi","year":"2019","unstructured":"Kazemi M, Khodaiyan F, Hosseini SS, Najari Z. An integrated valorization of industrial waste of eggplant: Simultaneous recovery of pectin, phenolics and sequential production of pullulan. Waste Manage. 2019;1(100):101\u201311.","journal-title":"Waste Manage"},{"key":"2536_CR26","first-page":"337","volume":"4","author":"AR Shet","year":"2018","unstructured":"Shet AR, Desai SV, Achappa S. Pectinolytic enzymes: classification, production, purification and applications. Res J Life Sci Bioinform Pharm Chem Sci. 2018;4:337\u201348.","journal-title":"Res J Life Sci Bioinform Pharm Chem Sci."},{"key":"2536_CR27","doi-asserted-by":"crossref","unstructured":"Bergey DH, Breed RS, Murray EGD, Hitchens AP. Bergey's Manual of Determinative Bacteriology. A Key for the Identification of Organisms of the Class Schizomycetes. Bergey's Manual of Determinative Bacteriology. A Key for the Identification of Organisms of the Class Schizomycetes., (Edn 5). 1939.","DOI":"10.1097\/00010694-193910000-00035"},{"key":"2536_CR28","first-page":"632","volume":"3","author":"PK Hitha","year":"2014","unstructured":"Hitha PK, Girija D. Isolation and screening of native microbial isolates for pectinase activity. Int J Sci Res (Ahmedabad). 2014;3:632\u20134.","journal-title":"Int J Sci Res (Ahmedabad)."},{"issue":"2","key":"2536_CR29","first-page":"636","volume":"30","author":"LK Varghese","year":"2013","unstructured":"Varghese LK, Rizvi AF, Gupta AK. Isolation, screening and biochemical characterization of pectinolytic microorganism from soil sample of Raipur city. J Biol Chem Res. 2013;30(2):636\u201343.","journal-title":"J Biol Chem Res."},{"key":"2536_CR30","doi-asserted-by":"publisher","unstructured":"Obafemi YD, Ajayi AA, Taiwo OS, Olorunsola SJ, Isibor PO. Isolation of polygalacturonase-producing bacterial strain from tomatoes (Lycopersicon esculentum Mill.). Int J Microbiol. 2019:7505606. https:\/\/doi.org\/10.1155\/2019\/7505606.","DOI":"10.1155\/2019\/7505606"},{"key":"2536_CR31","doi-asserted-by":"publisher","unstructured":"Siddiqui M, Pande V, Arif M. Production, purification, and characterization of polygalacturonase from Rhizomucor pusillus isolated from decomposting orange peels. Enzyme Res. 2012;2012:138634. https:\/\/doi.org\/10.1155\/2012\/138634.\u00a0","DOI":"10.1155\/2012\/138634"},{"issue":"3","key":"2536_CR32","first-page":"238761","volume":"2","author":"OJ Oumer","year":"2017","unstructured":"Oumer OJ. Pectinase: substrate, production and their biotechnological applications. Int J Environ Agric Biotechnol. 2017;2(3).","journal-title":"Int J Environ Agric Biotechnol."},{"key":"2536_CR33","doi-asserted-by":"publisher","first-page":"743","DOI":"10.21786\/bbrc\/11.4\/27","volume":"11","author":"S Mahalik","year":"2018","unstructured":"Mahalik S, Mohapatra D, Kumar D. Cellulase production in Lysinibacillus sp isolated from the estuaries of Odisha. Biosci Biotechnol Res Commun. 2018;11:743\u201353.","journal-title":"Biosci Biotechnol Res Commun."},{"issue":"2","key":"2536_CR34","first-page":"80","volume":"4","author":"SJ Sareen","year":"2018","unstructured":"Sareen SJ, Thomas S, Sruthy AJ. Molecular characterization of microorganisms in municipal solid waste for production of industrial enzymes and enhanced biodegradation. Biotechnol Res. 2018;4(2):80\u20137.","journal-title":"Biotechnol Res."},{"key":"2536_CR35","doi-asserted-by":"publisher","first-page":"64","DOI":"10.7150\/jgen.38365","volume":"7","author":"N Hugouvieux-Cotte-Pattat","year":"2019","unstructured":"Hugouvieux-Cotte-Pattat N, Jacot-des-Combes C, Briolay J. Genomic characterization of a pectinolytic isolate of Serratia oryzae isolated from lake water. J Genomics. 2019;7:64.","journal-title":"J Genomics."},{"issue":"1","key":"2536_CR36","doi-asserted-by":"publisher","first-page":"110","DOI":"10.17113\/ftb.56.01.18.5477","volume":"56","author":"A Mohandas","year":"2018","unstructured":"Mohandas A, Raveendran S, Parameswaran B, Abraham A, Athira RS, Mathew AK, Pandey A. Production of pectinase from Bacillus sonorensis MPTD1. Food Technol Biotechnol. 2018;56(1):110.","journal-title":"Food Technol Biotechnol."},{"issue":"1","key":"2536_CR37","doi-asserted-by":"publisher","first-page":"11","DOI":"10.17582\/pujz\/2018.33.1.11.15","volume":"33","author":"F Tabssum","year":"2018","unstructured":"Tabssum F, Ali SS. Screening of pectinase producing gram positive bacteria: isolation and characterization. Punjab Univ J Zool. 2018;33(1):11\u20135.","journal-title":"Punjab Univ J Zool."},{"issue":"9","key":"2536_CR38","doi-asserted-by":"publisher","first-page":"2931","DOI":"10.1016\/j.procbio.2005.03.026","volume":"40","author":"RS Jayani","year":"2005","unstructured":"Jayani RS, Saxena S, Gupta R. Microbial pectinolytic enzymes: a review. Process Biochem. 2005;40(9):2931\u201344.","journal-title":"Process Biochem."},{"issue":"1","key":"2536_CR39","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/2193-1801-3-327","volume":"3","author":"AM Embaby","year":"2014","unstructured":"Embaby AM, Masoud AA, Marey HS, Shaban NZ, Ghonaim TM. Raw agro-industrial orange peel waste as a low cost effective inducer for alkaline polygalacturonase production from Bacillus licheniformis SHG10. Springerplus. 2014;3(1):1\u20133. https:\/\/doi.org\/10.1186\/2193-1801-3-327.","journal-title":"Springerplus"},{"key":"2536_CR40","doi-asserted-by":"publisher","unstructured":"Sohail M, Latif Z. Phylogenetic analysis of polygalacturonase-producing Bacillus and Pseudomonas isolated from plant waste material. Jundishapur J Microbiol.\u00a0 2016;9(1):e28594. https:\/\/doi.org\/10.5812\/jjm.28594.","DOI":"10.5812\/jjm.28594"},{"issue":"1","key":"2536_CR41","first-page":"981","volume":"4","author":"MN Kothari","year":"2013","unstructured":"Kothari MN, Baig MM. Production and characterization of extracellular polygalacturonase by Erwinia carotovora MTCC 1428. Int J Adv Biotechnol Res. 2013;4(1):981\u201398.","journal-title":"Int J Adv Biotechnol Res."},{"issue":"1","key":"2536_CR42","doi-asserted-by":"publisher","first-page":"131","DOI":"10.2306\/scienceasia1513-1874.2007.33.131","volume":"33","author":"C Sittidilokratna","year":"2007","unstructured":"Sittidilokratna C, Suthirawut S, Chitradon L, Punsuvon V, Vaithanomsat P, Siriacha P. Screening of pectinase producing bacteria and their efficiency in biopulping of paper mulberry bark. Sci Asia. 2007;33(1):131\u20135 0.2306\/scienceasia1513\u22121874.2007.33.131.","journal-title":"Sci Asia."},{"key":"2536_CR43","doi-asserted-by":"publisher","unstructured":"Karabi R, Sujan D, Uddin MK, Rasel B, Hossain MT. Extracellular pectinase from a novel bacterium Chryseobacterium indologenes strain SD and its application in fruit juice clarification. Enzyme Res. 2018;2018:3859752. https:\/\/doi.org\/10.1155\/2018\/3859752.","DOI":"10.1155\/2018\/3859752"},{"issue":"6","key":"2536_CR44","doi-asserted-by":"publisher","first-page":"3579","DOI":"10.1007\/s13197-014-1413-8","volume":"52","author":"S Barman","year":"2015","unstructured":"Barman S, Sit N, Badwaik LS, Deka SC. Pectinase production by Aspergillus niger using banana (Musa balbisiana) peel as substrate and its effect on clarification of banana juice. J Food Sci Technol. 2015;52(6):3579\u201389. https:\/\/doi.org\/10.1007\/s13197-014-1413-8.","journal-title":"J Food Sci Technol"},{"issue":"6","key":"2536_CR45","first-page":"1","volume":"4","author":"D Tambekar","year":"2016","unstructured":"Tambekar D, Tambekar S, Rajgire A, Jadhav A, Sawale K. Isolation and characterization of amylase from Lysinibacillus xylanilyticus from alkaline environment. Int J Res Stud Biosci. 2016;4(6):1\u20134.","journal-title":"Int J Res Stud Biosci."},{"issue":"1","key":"2536_CR46","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1590\/0001-3765201620140136","volume":"88","author":"PR Campos","year":"2016","unstructured":"Campos PR, Modenes AN, Espinoza-Quinones FR, Trigueros DE, Barros ST, Pereira NC. Improvement on the concentrated grape juice physico-chemical characteristics by an enzymatic treatment and Membrane Separation Processes. An Acad Bras Ci\u00eanc. 2016 Mar;88(1):423\u201336.","journal-title":"An Acad Bras Ci\u00eanc"},{"key":"2536_CR47","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1590\/S0001-37141999000400002","volume":"30","author":"MM Soares","year":"1999","unstructured":"Soares MM, Silva RD, Gomes E. Screening of bacterial strains for pectinolytic activity: characterization of the polygalacturonase produced by Bacillus sp. Rev Microbiol. 1999;30:299\u2013303. https:\/\/doi.org\/10.1590\/S0001-37141999000400002.","journal-title":"Rev Microbiol"},{"issue":"1","key":"2536_CR48","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1007\/s00217-014-2198-8","volume":"239","author":"N Demir","year":"2014","unstructured":"Demir N, Nadaroglu H, Demir Y, Isik C, Taskin E, Adiguzel A, Gulluce M. Purification and characterization of an alkaline pectin lyase produced by a newly isolated Brevibacillus borstelensis (P35) and its applications in fruit juice and oil extraction. Eur Food Res Technol. 2014;239(1):127\u201335. https:\/\/doi.org\/10.1007\/s00217-014-2198-8.","journal-title":"Eur Food Res Technol"},{"issue":"1","key":"2536_CR49","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.fbp.2013.07.003","volume":"92","author":"S Maktouf","year":"2014","unstructured":"Maktouf S, Neifar M, Drira SJ, Baklouti S, Fendri M, Ch\u00e2abouni SE. Lemon juice clarification using fungal pectinolytic enzymes coupled to membrane ultrafiltration. Food Bioprod Process. 2014;92(1):14\u20139. https:\/\/doi.org\/10.1016\/j.fbp.2013.07.003.","journal-title":"Food Bioprod Process"},{"issue":"1","key":"2536_CR50","first-page":"1","volume":"4","author":"A Kumar","year":"2012","unstructured":"Kumar A, Sharma R. Production of alkaline pectinase by bacteria (Cocci sps.) isolated from decomposing fruit materials. J Phytol. 2012;4(1):1\u20135.","journal-title":"J Phytol."},{"issue":"4","key":"2536_CR51","doi-asserted-by":"publisher","first-page":"1369","DOI":"10.1016\/j.foodchem.2011.05.065","volume":"129","author":"P Yuan","year":"2011","unstructured":"Yuan P, Meng K, Huang H, Shi P, Luo H, Yang P, Yao B. A novel acidic and low-temperature-active endo-polygalacturonase from Penicillium sp. CGMCC 1669 with potential for application in apple juice clarification. Food Chemistry. 2011;129(4):1369\u201375.","journal-title":"Food Chemistry."},{"issue":"23","key":"2536_CR52","doi-asserted-by":"publisher","first-page":"11471","DOI":"10.1021\/jf8019437","volume":"56","author":"N Diano","year":"2008","unstructured":"Diano N, Grimaldi T, Bianco M, Rossi S, Gabrovska K, Yordanova G, Godjevargova T, Grano V, Nicolucci C, Mita L, Bencivenga U. Apple juice clarification by immobilized pectolytic enzymes in packed or fluidized bed reactors. J Agric Food Chem. 2008;56(23):11471\u20137.","journal-title":"J Agric Food Chem"},{"issue":"137","key":"2536_CR53","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2020.110500","volume":"1","author":"SS Hosseini","year":"2021","unstructured":"Hosseini SS, Khodaiyan F, Mousavi SM, Azimi SZ. Clarification of the pomegranate juice in a bioreactor packed by pectinase enzymes immobilized on the glass bead activated with polyaldehyde polysaccharides. LWT. 2021;1(137): 110500.","journal-title":"LWT"},{"issue":"2","key":"2536_CR54","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/j.jgeb.2015.07.004","volume":"13","author":"HU Rehman","year":"2015","unstructured":"Rehman HU, Siddique NN, Aman A, Nawaz MA, Baloch AH, Qader SA. Morphological and molecular based identification of pectinase producing Bacillus licheniformis from rotten vegetable. Journal of Genetic Engineering and Biotechnology. 2015;13(2):139\u201344. https:\/\/doi.org\/10.1016\/j.jgeb.2015.07.004.","journal-title":"Journal of Genetic Engineering and Biotechnology"},{"key":"2536_CR55","doi-asserted-by":"publisher","unstructured":"Jayani RS, Shukla SK, Gupta R. Screening of bacterial strains for polygalacturonase activity: its production by Bacillus sphaericus (MTCC 7542). Enzyme Res. 2010;306785:5. https:\/\/doi.org\/10.4061\/2010\/306785.","DOI":"10.4061\/2010\/306785"},{"key":"2536_CR56","first-page":"4445","volume":"14","author":"S Savas","year":"2009","unstructured":"Savas S, Adiguzel A, Inan K, Ozkan H, Gulluce M, Sahin F. Molecular characterization of thermophilic bacteria isolated from Van City Ercis Town Hasanabdal hot spring. Rom Biotech Lett. 2009;14:4445\u201354.","journal-title":"Rom Biotech Lett."},{"key":"2536_CR57","doi-asserted-by":"crossref","unstructured":"Roy K, Dey S, Uddin M, Barua R, Hossain M. Extracellular pectinase from a novel bacterium Chryseobacterium indologenes strain SD and its application in fruit juice clarification. Enzyme Res. 2018;2018.","DOI":"10.1155\/2018\/3859752"},{"issue":"1","key":"2536_CR58","first-page":"853","volume":"4","author":"IG Khan","year":"2016","unstructured":"Khan IG, Barate DL. Effect of various parameters on activity of pectinase enzyme. Int J Adv Res. 2016;4(1):853\u201362.","journal-title":"Int J Adv Res."},{"key":"2536_CR59","doi-asserted-by":"publisher","first-page":"47","DOI":"10.3923\/jm.2016.47.55","volume":"11","author":"C Abiola","year":"2016","unstructured":"Abiola C, Oyetayo VO. Isolation and biochemical characterization of microorganisms associated with the fermentation of Kersting\u2019s groundnut (Macrotylomageocarpum). Res J Microbiol. 2016;11:47\u201355.","journal-title":"Res J Microbiol"},{"issue":"1","key":"2536_CR60","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1023\/A:1016667930174","volume":"17","author":"MM Soares","year":"2001","unstructured":"Soares MM, Da Silva R, Carmona EC, Gomes E. Pectinolytic enzyme production by Bacillus species and their potential application on juice extraction. World J Microbiol Biotechnol. 2001;17(1):79\u201382. https:\/\/doi.org\/10.1023\/A:1016667930174.","journal-title":"World J Microbiol Biotechnol"},{"issue":"12","key":"2536_CR61","doi-asserted-by":"publisher","first-page":"1185","DOI":"10.1002\/jobm.201900321","volume":"59","author":"M Koshy","year":"2019","unstructured":"Koshy M, De S. Effect of Bacillus tequilensis SALBT crude extract with pectinase activity on demucilation of coffee beans and juice clarification. J Basic Microbiol. 2019;59(12):1185\u201394.","journal-title":"J Basic Microbiol."},{"issue":"3","key":"2536_CR62","first-page":"48","volume":"5","author":"P Ramalingam","year":"2013","unstructured":"Ramalingam P, Aswini V, Pradeepa P, Sriram S, Swathi H. Partial purification and characterization of cellulase, pectinase and xylanase from Penicillium chrysogenum. IOSR J Environ Sci Toxicol Food Technol. 2013;5(3):48\u201359.","journal-title":"IOSR J Environ Sci Toxicol Food Technol."},{"issue":"3","key":"2536_CR63","doi-asserted-by":"publisher","first-page":"426","DOI":"10.1021\/ac60147a030","volume":"31","author":"GL Miller","year":"1959","unstructured":"Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem. 1959;31(3):426\u20138.","journal-title":"Anal Chem"}],"container-title":["BMC Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12866-022-02536-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12866-022-02536-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12866-022-02536-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,24]],"date-time":"2022-05-24T02:10:36Z","timestamp":1653358236000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcmicrobiol.biomedcentral.com\/articles\/10.1186\/s12866-022-02536-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,24]]},"references-count":63,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["2536"],"URL":"https:\/\/doi.org\/10.1186\/s12866-022-02536-8","relation":{},"ISSN":["1471-2180"],"issn-type":[{"value":"1471-2180","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,24]]},"assertion":[{"value":"3 March 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 April 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 May 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"There was no human or animal participation in the research, and no data was collected. With the company's permission, a sample was taken from ECOPIA PLC in Addis Ababa, Ethiopia.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"145"}}