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These involve several bacterial clones with multidrug resistance making them difficult to treat. The aims of this study was to perform molecular typing of <jats:italic>E. coli<\/jats:italic> strains using universal primer (GTG)<jats:sub>5<\/jats:sub>. In this study, 53 <jats:italic>E. coli<\/jats:italic> strains were collected from inpatients and outpatients. The test of antimicrobial sensibility was realized using CA-SFM \/EUCAST method and strains were identified by conventional microbiological tests. The carbapenemase-producing strains were demonstrated by phenotypic method. Bacterial DNA was extracted by boiling method. (GTG)<jats:sub>5<\/jats:sub>-PCR was used for strain subtyping. The DendroUPGMA software was used for grouping of strains from the genetic fingerprints obtained by (GTG)<jats:sub>5<\/jats:sub>-PCR.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Antibiotic susceptibility test revealed that all strains were multi-drug resistant (MDR). Its strains showed resistance to at least three different families of antibiotics. Of this MDR strains, only one was a metallo-\u03b2-lactamase producer. The dendrogram obtained using genetic fingerprinting allowed the <jats:italic>E. coli<\/jats:italic> strains to be grouped into 22 clusters (G1 to G22).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>The (GTG) 5-PCR assay enabled rapid molecular typing of <jats:italic>E. coli<\/jats:italic> strains. The strains of <jats:italic>E. coli<\/jats:italic> typed in this study would belong to different clones.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-022-02537-7","type":"journal-article","created":{"date-parts":[[2022,4,29]],"date-time":"2022-04-29T07:02:55Z","timestamp":1651215775000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["(GTG)5-PCR fingerprinting of multi-drug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina Faso"],"prefix":"10.1186","volume":"22","author":[{"given":"Boukar\u00e9","family":"Kabor\u00e9","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ganam\u00e9 Abasse","family":"Ou\u00e9draogo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hama","family":"Ciss\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henri S.","family":"Ou\u00e9draogo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuel","family":"Sampo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koudbi Jacob","family":"Zongo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boukar\u00e9","family":"Zeba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yves","family":"Traor\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Gnankin\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Idrissa","family":"Sanou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aly","family":"Savadogo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,4,29]]},"reference":[{"issue":"11","key":"2537_CR1","doi-asserted-by":"publisher","first-page":"950","DOI":"10.1051\/medsci\/20102611950","volume":"26","author":"P Nordmann","year":"2010","unstructured":"Nordmann P. 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