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The use of substances produced by bees has evidenced the antimicrobial action in different types of organisms. Thus, the use of bacteria isolated from larval food of stingless bees opens the way for the identification of the new molecules. The effect of supernatants produced by these bacteria was evaluated for their ability to inhibit the growth of bacteria of clinical interest. Furthermore, their effects were evaluated when used in synergy with antibiotics available in the pharmaceutical industry.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>A few supernatants showed an inhibitory effect against susceptible and multiresistant strains in the PIC assay and the modulation assay. Emphasizing the inhibitory effect on multidrug-resistant strains, 7 showed an effect on multidrug-resistant <jats:italic>Escherichia coli<\/jats:italic> (APEC), <jats:italic>Klebsiella pneumoniae<\/jats:italic> carbapenemase (KPC), multidrug-resistant <jats:italic>Pseudomonas aeruginosa<\/jats:italic>, and multidrug-resistant <jats:italic>Staphylococcus aureus<\/jats:italic> (MRSA) in the PIC assay. Of the supernatants analyzed, some presented synergism for more than one species of multidrug-resistant bacteria. Nine had a synergistic effect with ampicillin on <jats:italic>E. coli<\/jats:italic> (APEC) or <jats:italic>S. aureus<\/jats:italic> (MRSA), 5 with penicillin G on <jats:italic>E. coli<\/jats:italic> (APEC) or KPC, and 3 with vancomycin on KPC.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>In summary, the results indicate that supernatants produced from microorganisms can synthesize different classes of molecules with potent antibiotic activity against multiresistant bacteria. Thus, suggesting the use of these microorganisms for use clinical tests to isolate the molecules produced and their potential for use.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-022-02548-4","type":"journal-article","created":{"date-parts":[[2022,5,12]],"date-time":"2022-05-12T05:02:54Z","timestamp":1652331774000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Antimicrobial activity of supernatants produced by bacteria isolated from Brazilian stingless bee\u2019s larval food"],"prefix":"10.1186","volume":"22","author":[{"given":"Ana Carolina Costa","family":"Santos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serena Mares","family":"Malta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel Cristina Cavalcanti","family":"Dantas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina Dias","family":"Coelho Rocha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasco","family":"Ariston de Carvalho Azevedo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Ueira-Vieira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,5,12]]},"reference":[{"key":"2548_CR1","doi-asserted-by":"publisher","unstructured":"Blair JMA, Webber MA, Baylay AJ, Ogbolu DO, Piddock LJV. 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