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The insect gut harbors a variety of microorganisms that can live and multiply within it, thus contributing to digestion, nutrition, and development of its host. The composition of bacterial communities in mosquitoes can be influenced by both biotic and abiotic factors. The goal of this study was to investigate the bacterial diversity of <jats:italic>Hg. leucocelaenus<\/jats:italic> and verify the differences between the bacterial communities in <jats:italic>Hg. leucocelaenus<\/jats:italic> from three different locations in the Atlantic tropical rain forest and southeastern state of S\u00e3o Paulo State, Brazil.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The phylum <jats:italic>Proteobacteria<\/jats:italic> was found in mosquitoes collected from the three selected study sites. More than 50% of the contigs belong to <jats:italic>Wolbachia<\/jats:italic>, followed by 5% <jats:italic>Swaminathania<\/jats:italic>, and 3% <jats:italic>Acinetobacter<\/jats:italic>. The genus <jats:italic>Serratia<\/jats:italic> was found in samples from two locations.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p><jats:italic>Wolbachia<\/jats:italic> was reported for the first time in this species and may indicates that the vector competence of the populations of the species can vary along its geographical distribution area. The presence of <jats:italic>Serratia<\/jats:italic> might facilitate viral invasion caused by the disruption of the midgut barrier via action of the SmEnhancin protein, which digests the mucins present in the intestinal epithelium.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-022-02571-5","type":"journal-article","created":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T04:14:57Z","timestamp":1655871297000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Bacterial diversity in Haemagogus leucocelaenus (Diptera: Culicidae) from Vale do Ribeira, S\u00e3o Paulo, Brazil"],"prefix":"10.1186","volume":"22","author":[{"given":"Herculano","family":"da Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiane M. 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