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The combination of loop-mediated isothermal amplification (LAMP) and microfluidic technology was used to develop the LAMP-microfluidic chip for rapid, simple, sensitive and simultaneous detection of the five target pathogens above.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Standard strains and clinical isolates were used for the establishment of the novel LAMP method in tube and LAMP-microfluidic chip, followed by the chip detection on 103 clinical samples and PCR verification partially. The sensitivities of LAMP of <jats:italic>S<\/jats:italic>. <jats:italic>agalactiae<\/jats:italic>, <jats:italic>E<\/jats:italic>. <jats:italic>faecalis<\/jats:italic>, <jats:italic>G<\/jats:italic>. <jats:italic>vaginalis<\/jats:italic>, and <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> in tube were 22.0, 76.0, 13.2, 1.11\u00a0CFU\/\u03bcL, respectively, and <jats:italic>C<\/jats:italic>. <jats:italic>trachomatis<\/jats:italic> was 41.3 copies\/\u03bcL; on LAMP-microfluidic chip they were 260, 154, 3.9 and 7.53\u00a0CFU\/\u03bcL, respectively, and <jats:italic>C<\/jats:italic>. <jats:italic>trachomatis<\/jats:italic> was 120 copies\/\u03bcL. The positive coincidence rates of clinical stains in tube and on chip experiments were 100%. Compared with the classic culture method performed in hospitals, the positive coincidence rate of the 103 clinical samples detected by LAMP-microfluidic chip were 100%. For the six inconsistent ones, including four <jats:italic>G<\/jats:italic>. <jats:italic>vaginalis<\/jats:italic> and two <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> positive samples tested by LAMP-microfluidic chip and verified by PCR were negative by culturing method in hospitals, indicating the lack of efficient detection by the classic culturing method.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Our study suggested that the LAMP-microfluidic chips could simultaneously, efficiently, and accurately detect multiple main pathogens, including <jats:italic>S<\/jats:italic>. <jats:italic>agalactiae<\/jats:italic>, <jats:italic>E<\/jats:italic>. <jats:italic>faecalis<\/jats:italic>, <jats:italic>G<\/jats:italic>. <jats:italic>vaginalis<\/jats:italic>, <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> and <jats:italic>C<\/jats:italic>. <jats:italic>trachomatis<\/jats:italic>, in clinical samples of female RTI to give a great clinical value. Accordingly, this novel method has the potential to provide a valuable reference for female RTI screening and early diagnosis during pregnancy.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-022-02657-0","type":"journal-article","created":{"date-parts":[[2022,10,29]],"date-time":"2022-10-29T04:02:37Z","timestamp":1667016157000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Rapid and simultaneous detection of multiple pathogens in the lower reproductive tract during pregnancy based on loop-mediated isothermal amplification-microfluidic chip"],"prefix":"10.1186","volume":"22","author":[{"given":"Xiaofang","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiguo","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruolin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuting","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchen","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangjie","family":"Lao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huawei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxia","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenming","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qun","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,10,29]]},"reference":[{"key":"2657_CR1","doi-asserted-by":"publisher","unstructured":"Qie M, Deng Y. 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