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It poses a potential threat to patients via the production of \u03b2-lactamases, which decrease the efficacy of antimicrobial treatment and impair the management of its pathogenicity. Hence, this study was established to determine the prevalence of extended-spectrum \u03b2-lactamases (ESBLs), AmpC, and carbapenemases of<jats:italic>P. mirabilis<\/jats:italic>isolated from various clinical specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>Proteus mirabilis<\/jats:italic>was identified in 20.7% (58\/280) of specimens. ESBL producers were present at a rate of 51.7% (30\/58). All AmpC-positive isolates (<jats:italic>n<\/jats:italic>\u2009=\u200920) produced ESBLs as well, so 66.7% of ESBL-producing isolates coproduced AmpC enzymes. The modified Hodge test confirmed carbapenemase production in six out of seven imipenem nonsusceptible isolates. Of these, only two (5.7%) isolates were also ESBL-and AmpC-positive. Antibiotic resistance reached the highest level for cotrimoxazole (62.1%,<jats:italic>n<\/jats:italic>\u2009=\u200936\/58 isolates) and the lowest for imipenem (12.1%,<jats:italic>n<\/jats:italic>\u2009=\u20097\/58 isolates). The levels of multidrug-resistant (MDR) was 41.4% among the tested isolates. The<jats:italic>bla<\/jats:italic><jats:sub>SHV<\/jats:sub>(83.3%),<jats:italic>bla<\/jats:italic><jats:sub>AmpC<\/jats:sub>(80%), and<jats:italic>bla<\/jats:italic><jats:sub>VIM-1<\/jats:sub>(50%) were the most detected genes in phenotypically confirmed ESBL-, AmpC-, and carbapenemase-producing isolates, respectively. Besides, more than a half of the tested<jats:italic>P. mirabilis<\/jats:italic>strains (53%) coproduced ESBLs and AmpC. Moreover, two isolates coproduced ESBLs and AmpC together with carbapenemases. Furthermore, dendrogram analysis showed great genetic divergence\u00a0based on the 21 different enterobacterial repetitive intergenic consensus (ERIC) patterns (P1\u2013P21) through the 34 \u03b2-lactamase producers. ERIC analysis distinguished clonal similarities between isolates 21 and 22 in P2 and 9 and 10 in P4, which were isolated from the same clinical source and possessed similar patterns of \u03b2-lactamase-encoding genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Hence, there is an urgent need to monitor hospitalized patients and improve healthcare in order to reduce the incidence of infection and outbreaks of infection with antibiotic-resistant<jats:italic>Proteus<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12866-022-02662-3","type":"journal-article","created":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T11:03:38Z","timestamp":1665486218000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Prevalence of extended-spectrum \u03b2-lactamases, AmpC, and carbapenemases in Proteus mirabilis clinical isolates"],"prefix":"10.1186","volume":"22","author":[{"given":"Mona","family":"Shaaban","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soha Lotfy","family":"Elshaer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ola A.","family":"Abd El-Rahman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,10,11]]},"reference":[{"key":"2662_CR1","first-page":"341","volume":"135","author":"J Kishore","year":"2012","unstructured":"Kishore J. 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