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Recently, new research lines employed nano-drug delivery systems to enhance antibacterial activity of the existing antibiotics. Accordingly, the objective of this study is to optimize surfactant nanovesicles to improve the antimicrobial effect of meropenem, ertapenem and tigecycline against Carbapenemase Resistant <jats:italic>Enterobacteriaceae<\/jats:italic> (CRE) and extended spectrum beta-lactamases producing bacteria (ESBL). <jats:italic>Klebsiella pneumoniae<\/jats:italic> and <jats:italic>Escherichia coli<\/jats:italic> were used as the test organisms. <jats:italic>In vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> evaluations were conducted to prove the efficacy of niosome-encapsulated drugs formulations. The results revealed that surfactant vesicles were able to reduce the MIC values of the tested drugs by nine-fold change compared to their free forms. Scanning Electron Microscope (SEM) showed possible adhesion\/fusion of the vesicles encapsulated drugs on the bacterial cells compared to its solution. <jats:italic>In vivo<\/jats:italic> investigations using animal skin model confirmed the superiority of nanovesicles drug encapsulation regarding both wound size and histopathological examination. Wound surface area was reduced from 24.6mm<jats:sup>2<\/jats:sup> in absence of drug to reach 13.9, and 6.2mm<jats:sup>2<\/jats:sup> in presence of ertapenem solution or niosomes, respectively. Nanovesicular formulations can be considered as effective drug delivery systems that can diminish bacterial resistance against \u03b2-lactams antibiotics.<\/jats:p>","DOI":"10.1186\/s12866-023-02812-1","type":"journal-article","created":{"date-parts":[[2023,3,26]],"date-time":"2023-03-26T20:39:05Z","timestamp":1679863145000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Surfactant nanovesicles for augmented antibacterial activity against carbapenemase resistant enterobacteriaceae and extended spectrum beta-lactamases producing bacteria: in vitro and in vivo evaluation"],"prefix":"10.1186","volume":"23","author":[{"given":"Amal M.","family":"Abo Kamer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noha M.","family":"Amer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed A.","family":"Abdelmegeed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gamal M.","family":"El Maghraby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noha M.","family":"Gamaleldin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,3,16]]},"reference":[{"key":"2812_CR1","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/S1473-3099(08)70041-0","volume":"8","author":"JD Pitout","year":"2008","unstructured":"Pitout JD, Laupland KB. 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The samples were collected as bacterial isolates grown on selected media plates and identified with VITEK<sup>\u00ae<\/sup> 2 system from the microbiology laboratory of Clinical Pathology Department, International Medical Center, Cairo, Egypt.The study protocol and animal treatment (<i>In vivo<\/i> investigation) were approved by the Research Ethics committee at Faculty of Pharmacy \u2013 The British University in Egypt which is approved by ENERC (Egyptian Network of Research Ethics Committees) with serial number EX-2001.All <i>In vivo<\/i> methods were carried out in accordance with relevant guidelines and regulations. All methods were reported accordance with ARRIVE guidelines for the reporting of animal experiments.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent of participate"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}}],"article-number":"73"}}