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A variety of benefits are provided by these bacteria to human and insect hosts, including protection from viruses and parasites and the ability to kill males. In this study, <jats:italic>Wolbachia<\/jats:italic> was identified in <jats:italic>Aedes aegypti<\/jats:italic> present in Jeddah, Saudi Arabia. A population of mosquitoes was collected from eight different areas, processed, and tested for <jats:italic>Wolbachia<\/jats:italic> using 16\u00a0S rRNA specific to <jats:italic>Wolbachia<\/jats:italic> bacteria and <jats:italic>Wolbachia<\/jats:italic> surface protein (<jats:italic>wsp<\/jats:italic>) under optimized PCR conditions. In five ecologically diverse sites to determine <jats:italic>Wolbachia<\/jats:italic> prevalence, we identified eleven diverse novel resident <jats:italic>Wolbachia<\/jats:italic> strains within <jats:italic>Ae. Aegypti<\/jats:italic> for the first time in Jeddah, Saudi Arabia. Future studies to evaluate the possible use of <jats:italic>Wolbachia<\/jats:italic> as a control agent in <jats:italic>Aedes<\/jats:italic> sp. in Saudi Arabia are necessary. <jats:italic>Wolbachia<\/jats:italic> prevalence rates and strain characterization through Sanger sequencing with multilocus sequence typing (MLST) and phylogenetic analysis revealed significant diversity. In developing biocontrol strategies, it is beneficial to consider the implications of resident <jats:italic>Wolbachia<\/jats:italic> strains.<\/jats:p>","DOI":"10.1186\/s12866-023-03010-9","type":"journal-article","created":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T11:01:59Z","timestamp":1696590119000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Identification of Wolbachia new strains from Aedes aegypti mosquitoes, the vector of dengue fever in Jeddah Province"],"prefix":"10.1186","volume":"23","author":[{"given":"E. 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Mosquito-borne human viral Diseases: why Aedes aegypti? Am J Trop Med Hyg. 2018;98(6):1563\u20135.","journal-title":"Am J Trop Med Hyg"},{"issue":"12","key":"3010_CR5","doi-asserted-by":"publisher","first-page":"103448","DOI":"10.1016\/j.sjbs.2022.103448","volume":"29","author":"HSA Rashidi","year":"2022","unstructured":"Rashidi HSA, Al-Otaibi WM, Alghamdi KM, Mahyoub JA. Effects of Blood Meal sources on the Biological characteristics of Aedes aegypti and Culex pipiens (Diptera: Culicidae). Saudi J Biol Sci. 2022;29(12):103448.","journal-title":"Saudi J Biol Sci"},{"issue":"03","key":"3010_CR6","doi-asserted-by":"publisher","first-page":"507","DOI":"10.13005\/bbra\/2854","volume":"17","author":"MA Alkuriji","year":"2020","unstructured":"Alkuriji MA, Al-Fageeh MB, Shaher FM, Almutairi BF. Dengue Vector Control: a review for Wolbachia-Based strategies. 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