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The association between SCC<jats:italic>mec<\/jats:italic> typing with <jats:italic>psm-mec<\/jats:italic> positivity in <jats:italic>staphylococci<\/jats:italic> and PD-related infections has not been identified. We aim to investigate the molecular epidemiology of CNS isolated from PD-peritonitis in a single Chinese center, focusing on the genetic determinants conferring methicillin resistance.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>We collected 10 genetically unrelated CNS isolates from 10 patients with CNS PD-related peritonitis. The patients were divided into two groups based on the results of MIC to oxacillin: the methicillin-resistant CNS (MRCNS) and methicillin-sensitive CNS (MSCNS) groups. The biofilm formation group (BFG) and the non-biofilm formation group (NBFG) were used as the control groups. Phenotypic and molecular methods were used to analyze SCC<jats:italic>mec<\/jats:italic> types I, II and III, associated genes and biofilm formation and the existence of <jats:italic>psm-mec<\/jats:italic>. The demographic data and clinical indicators were collected.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Ten CNS PD-related peritonitis patients were enrolled for this study. There were 6 MRCNS and 4 MRCNS isolates. SCC<jats:italic>mec<\/jats:italic> types were fully determined in 10 isolates. Seven <jats:italic>staphylococci<\/jats:italic> (70%) carried SCC<jats:italic>mec<\/jats:italic>, of which 4 isolates carried single SCC<jats:italic>mec<\/jats:italic> type I (40%) and 3 isolates had multiple SCC<jats:italic>mec<\/jats:italic> elements (I\u2009+\u2009III). Of the 6 MRCNS isolates, 3 carried SCC<jats:italic>mec<\/jats:italic> type I (50%) and 2 isolates carried SCC<jats:italic>mec<\/jats:italic> type I\u2009+\u2009III (33.3%). A high diversity of <jats:italic>ccr<\/jats:italic> types, <jats:italic>mec<\/jats:italic> complexes and <jats:italic>ccr-mec<\/jats:italic> complex combinations was identified among the 10 CNS isolates. The <jats:italic>psm-mec<\/jats:italic> gene was detected in 2\/10 (20%) CNS isolates. There was no mutation in the <jats:italic>psm-mec<\/jats:italic> gene.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The majority of isolates were hospital-associated isolates. Furthermore, 2 <jats:italic>psm-mec<\/jats:italic> positive isolates were MRCNS in the NBFG. The PD patients frequent exposure to hospital would be the main risk factor. The presence of the <jats:italic>psm-mec<\/jats:italic> signal in the spectra of the MRCNS tested here demonstrates the presence of certain SCC<jats:italic>mec<\/jats:italic> cassettes that convey methicillin resistance.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-023-03017-2","type":"journal-article","created":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T05:01:51Z","timestamp":1695445311000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification and characterization of SCCmec typing with psm-mec positivity in staphylococci from patients with coagulase-negative staphylococci peritoneal dialysis-related peritonitis"],"prefix":"10.1186","volume":"23","author":[{"given":"Jun","family":"Zhou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuishun","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjuan","family":"Lei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Man","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingli","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanqiong","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2023,9,23]]},"reference":[{"issue":"3","key":"3017_CR1","first-page":"47","volume":"42","author":"P Dzekova-Vidimliski","year":"2021","unstructured":"Dzekova-Vidimliski P, Nikolov IG, Gjorgjievski N, Selim G, Trajceska L, Stojanoska A, Rambabova-Bushljetik I, Simeonov R, Stojkovski L. 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