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N protein-derived peptides identified using a bioinformatics approach can potentially be used to develop a new generation of vaccines or diagnostic methods for detecting SARS-CoV-2 and its variants. However, further studies must demonstrate their capacity to be naturally processed by the immune system.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Objective<\/jats:title>\n                <jats:p>We aimed to examine the in vivo processing and recognition of <jats:italic>in silico<\/jats:italic>-identified peptides using the serum of immunized animals with the complete protein.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Recombinant N (Nrec) protein was subcutaneously administered to six Balb\/c mice. Enzyme-linked immunosorbent assay (ELISA), western blotting, dot blotting, and immunoprecipitation were performed to evaluate the recognition of the complete protein and <jats:italic>in silico-<\/jats:italic>derived peptides.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The serum of immunized mice recognized\u2009~\u200962.5 ng\/\u00b5L of Nrec with high specificity to linear and conformational epitopes. Dot blot analysis showed that peptides Npep2 and Npep3 were the most reactive.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Our data confirm the high immunogenicity of the SARS-CoV-2\u00a0N protein and provide evidence on the antigenicity of two peptides located in the N-arm\/RNA-binding domain (Npep2) and oligomerization domain\/C-tail (Npep3), considered the biologically active site of the N protein.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-023-03076-5","type":"journal-article","created":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T07:02:37Z","timestamp":1698476557000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Immunized mice naturally process in silico-derived peptides from the nucleocapsid of SARS-CoV-2"],"prefix":"10.1186","volume":"23","author":[{"given":"Mario Aldair","family":"Campos-Ru\u00edz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Berenice","family":"Illades-Aguiar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar","family":"Del Moral-Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Romo-Castillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela","family":"Salazar-Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nica","family":"Espinoza-Rojo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amalia","family":"Vences-Vel\u00e1zquez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Cort\u00e9s-Sarabia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor M.","family":"Luna-Pineda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,10,28]]},"reference":[{"key":"3076_CR1","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1016\/S0140-6736(20)30185-9","volume":"395","author":"C Wang","year":"2020","unstructured":"Wang C, Horby PW, Hayden FG, Gao GF. 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This study was approved by the Research Committee of the Health State Services with register number 10070121 from the Universidad Aut\u00f3noma de Guerrero. In contrast, for Hospital Infantil de Mexico Federico Gomez, the study was reviewed and approved by the Research Committee (Dr. Juan Gardu\u00f1o Espinosa), Ethics Committee (Dr. Miguel \u00c1ngel Gaxiola Garc\u00eda), and Biosecurity Committee (Dr. Marcela Salazar Garc\u00eda) of HIMFG, with permit numbers HIM\/2020\/029 SSA.11664.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"319"}}