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In this study we used whole genome sequencing to characterize the clonal composition, virulence and resistance genetic determinants of 58 <jats:italic>Staphylococcus\/Mammaliicoccus<\/jats:italic> spp. isolates from contralateral healthy skin and DFU from 44 hospitalized patients.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p><jats:italic>S. aureus<\/jats:italic> (n\u2009=\u200932) and <jats:italic>S. epidermidis<\/jats:italic> (n\u2009=\u200910) isolates were recovered from both DFUs and healthy skin, whereas, <jats:italic>S. haemolyticus<\/jats:italic> (n\u2009=\u20098), <jats:italic>M. sciuri<\/jats:italic> (n\u2009=\u20091), <jats:italic>S. hominis<\/jats:italic> (n\u2009=\u20091) and <jats:italic>S. simulans<\/jats:italic> (n\u2009=\u20093) were recovered exclusively from healthy skin. In contrast, <jats:italic>S. caprae<\/jats:italic> (n\u2009=\u20092) and <jats:italic>S. saprophyticus<\/jats:italic> (n\u2009=\u20091) were recovered only from DFUs. Among <jats:italic>S. aureus<\/jats:italic> isolates, MRSA were present with high prevalence (27\/32, 84.4%), 18 of which (66.7%) were from DFUs and 9 (33.3%) from healthy skin. In contrast, the coagulase-negative <jats:italic>Staphylococcus<\/jats:italic> (CoNS)\/<jats:italic>Mammaliicoccus<\/jats:italic> isolates (n\u2009=\u200926), in particular <jats:italic>S. epidermidis<\/jats:italic> and <jats:italic>S. haemolyticus<\/jats:italic> were more prevalent in healthy skin, (10\/26, 38.5%) and (8\/26, 30.8%), respectively. MLST, spa and SCC<jats:italic>mec<\/jats:italic> typing classified the 32 <jats:italic>S. aureus<\/jats:italic> isolates into 6 STs, ST672, ST80, ST241, ST1, ST97, ST291 and 4 unknown STs (STNF); 8 spa types, t044, t037, t3841, t1247, t127, t639, t937 and t9432 and 2 SCC<jats:italic>mec<\/jats:italic> types, type IV and type III(A). Among CoNS, the <jats:italic>S. epidermidis<\/jats:italic> isolates belonged to ST54, ST35 and ST640. <jats:italic>S. haemolyticus<\/jats:italic> belonged to ST3, ST25, ST29, ST1 and ST56. The sole <jats:italic>M. sciuri<\/jats:italic> isolate was found to carry an SCC<jats:italic>mec<\/jats:italic> type III(A). A wide range of virulence genes and antimicrobial resistance genes were found among our isolates, with varying distribution between species or STs. The pan-genome analysis revealed a highly clonal population of <jats:italic>Staphylococcus<\/jats:italic> isolates, particularly among <jats:italic>S. aureus<\/jats:italic> isolates. Interestingly, the majority of <jats:italic>S. aureus<\/jats:italic> isolates including MRSA, recovered from the healthy skin and DFUs of the same patient belonged to the same clone and exhibited similar virulence\/resistance genotype.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Our study provides clinically relevant information on the population profile, virulence and antibiotic resistance of <jats:italic>Staphylococcus\/Mammaliicoccus<\/jats:italic> spp. in DFIs, which could serve as a basis for further studies on these as well as other groups of pathogens associated with DFIs.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-023-03087-2","type":"journal-article","created":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T07:01:53Z","timestamp":1700118113000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Whole genome sequencing and pan-genome analysis of Staphylococcus\/Mammaliicoccus spp. isolated from diabetic foot ulcers and contralateral healthy skin of Algerian patients"],"prefix":"10.1186","volume":"23","author":[{"given":"Nerdjes","family":"Ferhaoui","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rina","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsuyoshi","family":"Sekizuka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Kuroda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed","family":"Sebaihia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2023,11,16]]},"reference":[{"key":"3087_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/978-1-4614-5441-0_1","volume-title":"Diabetes","author":"K Kaul","year":"2012","unstructured":"Kaul K, Tarr JM, Ahmad S, Kohner EM, Chibber R. 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