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Selected lactic acid bacteria (LAB) isolated from fermented foods with probiotic potential were evaluated for various tests, including exopolysaccharide production, antibiotic susceptibility, acid and bile tolerance, antibacterial activity, and cell adhesion and cytotoxicity to gastric cell lines. Six selected LAB strains maintained their high viability under gastrointestinal conditions, produced high exopolysaccharides, showed no or less cytotoxicity, and adhered successfully to gastric cells. Furthermore, three strains,\n                    <jats:italic>Weissella confusa<\/jats:italic>\n                    CYLB30,\n                    <jats:italic>Lactiplantibacillus plantarum<\/jats:italic>\n                    CYLB47, and\n                    <jats:italic>Limosilactobacillus fermentum<\/jats:italic>\n                    CYLB55, demonstrated a strong antibacterial effect against drug-resistant\n                    <jats:italic>Escherichia coli, Klebsiella pneumoniae<\/jats:italic>\n                    ,\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    ,\n                    <jats:italic>Salmonella enterica<\/jats:italic>\n                    serovar Choleraesuis,\n                    <jats:italic>Enterococcus faecium<\/jats:italic>\n                    , and\n                    <jats:italic>Staphylococcus aureus.<\/jats:italic>\n                    Whole genome sequencing was performed on these three strains using the Nanopore platform; then, the results showed that all three strains did not harbor genes related to toxins, superantigens, and acquired antimicrobial resistance, in their genome. The bacteriocin gene cluster was found in CYLB47 genome, but not in CYLB30 and CYLB55 genomes. In SDS-PAGE, the extract of CYLB30 and CYLB47 bacteriocin-like inhibitory substance (BLIS) yielded a single band with a size of less than 10\u00a0kDa. These BLIS inhibited the growth and biofilm formation of drug-resistant\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    and methicillin-resistant\n                    <jats:italic>S. aureus<\/jats:italic>\n                    (MRSA), causing membrane disruption and inhibiting adhesion ability to human skin HaCaT cells. Moreover, CYLB30 and CYLB47 BLIS rescued the larvae after being infected with\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    and MRSA infections. In conclusion, CYLB30 and CYLB47 BLIS may be potential alternative treatment for multidrug-resistant bacteria infections.\n                  <\/jats:p>","DOI":"10.1186\/s12866-024-03245-0","type":"journal-article","created":{"date-parts":[[2024,3,11]],"date-time":"2024-03-11T06:02:11Z","timestamp":1710136931000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["Characterization of the broad-spectrum antibacterial activity of bacteriocin-like inhibitory substance-producing probiotics isolated from fermented foods"],"prefix":"10.1186","volume":"24","author":[{"given":"Tran Thi Dieu","family":"Thuy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsu-Feng","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl Jay Ballena","family":"Bregente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fong-Chi Annabelle","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei-Chun","family":"Tu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Yen","family":"Kao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,11]]},"reference":[{"key":"3245_CR1","doi-asserted-by":"crossref","unstructured":"Garcia E, Ly N, Diep JK, Rao GG. 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