{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T15:16:08Z","timestamp":1793114168599,"version":"4.5.0"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,7,8]],"date-time":"2024-07-08T00:00:00Z","timestamp":1720396800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,7,8]],"date-time":"2024-07-08T00:00:00Z","timestamp":1720396800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>ESBL-producing <jats:italic>Escherichia coli<\/jats:italic> pose a growing health risk in community and healthcare settings. We investigated the resistome, virulome, mobilome, and genetic relatedness of multidrug-resistant (MDR) <jats:italic>E. coli<\/jats:italic> isolates from patients and their environment in a Ghanaian teaching hospital.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Materials and methods<\/jats:title>\n                <jats:p>Twenty-three MDR ESBL-producing or carbapenem-resistant <jats:italic>E. coli<\/jats:italic> isolates from a collection of MDR Gram-negative bacteria (GNB) from patients and environments were selected for genomic analyses. Whole genome sequencing and bioinformatics tools were used to analyze genomic characteristics and phylogeny.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The prevalence and incidence of rectal carriage of ESBL <jats:italic>E. coli<\/jats:italic> among patients were 13.65% and 11.32% respectively. The \u03b2-lactamase genes, <jats:italic>bla<\/jats:italic><jats:sub>TEM\u22121B<\/jats:sub> (10 isolates) and <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2212M\u221215<\/jats:sub> (12 isolates) were commonly associated with IncFIB plasmid replicons and co-occurred with aminoglycoside, macrolide, and sulfamethoxazole\/trimethoprim resistance. Insertion sequences, transposons, and class I integrons were found with <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2212M\u221215<\/jats:sub>. Carriage and environmental isolates carried multiple virulence genes, with <jats:italic>ter<\/jats:italic>C being the most prevalent in 21 isolates. Seventeen sequence types (STs) were identified, including a novel ST (ST13846). Phylogenetic analysis grouped the isolates into four main clusters, with one outlier. High genetic relatedness was observed between two carriage isolates of ST940 and between a carriage isolate and an environmental isolate of ST648. Isolates with different STs, collected at different times and locations, also showed genetic similarities.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>We identified ESBL-producing <jats:italic>E. coli<\/jats:italic> with diverse genomic characteristics circulating in different hospital directorates. Clonal relatedness was observed among isolates from patients and the environment, as well as between different patients, suggesting transmission within and between sources.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-024-03406-1","type":"journal-article","created":{"date-parts":[[2024,7,8]],"date-time":"2024-07-08T09:11:42Z","timestamp":1720429902000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Genomic characterization of multi drug resistant ESBL-producing Escherichia coli isolates from patients and patient environments in a teaching hospital in Ghana"],"prefix":"10.1186","volume":"24","author":[{"given":"Esther Eyram","family":"Asare Yeboah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Agyepong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Mbanga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Gyamfi","family":"Amoako","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akebe Luther King","family":"Abia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arshad","family":"Ismail","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Owusu-Ofori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabiha Yusuf","family":"Essack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,7,8]]},"reference":[{"issue":"1","key":"3406_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13756-017-0208-x","volume":"6","author":"JA Ayukekbong","year":"2017","unstructured":"Ayukekbong JA, Ntemgwa M, Atabe AN. 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