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The aim of this study was to assess the occurrence of coagulase-negative\n                      <jats:italic>Staphylococcus<\/jats:italic>\n                      species in bovine milk and dairy farms in Northwestern Ethiopia and to provide information about their antibiotic susceptibility and virulence gene profiles.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The cross-sectional study was conducted from February to August 2022. Coagulase-negative\n                      <jats:italic>Staphylococcus<\/jats:italic>\n                      species were isolated from 290 milk samples. Species isolation and identification were performed by plate culturing and biochemical tests and the antimicrobial susceptibility pattern of each isolate was determined by the Kirby-Bauer disc diffusion test. The single-plex PCR was used to detect the presence of virulent genes. The STATA software version 16 was used for data analysis. The prevalence, proportion of antimicrobial resistance and the number of virulent genes detected from coagulase-negative\n                      <jats:italic>Staphylococcus<\/jats:italic>\n                      species were analyzed using descriptive statistics.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Coagulase-negative\n                      <jats:italic>Staphylococcus<\/jats:italic>\n                      species were isolated in 28.6%, (95% CI: 23.5\u201334.2) of the samples. Of these, the\n                      <jats:italic>S. epidermidis<\/jats:italic>\n                      ,\n                      <jats:italic>S. sciuri<\/jats:italic>\n                      ,\n                      <jats:italic>S. warneri<\/jats:italic>\n                      ,\n                      <jats:italic>S. haemolyticus<\/jats:italic>\n                      ,\n                      <jats:italic>S. simulans<\/jats:italic>\n                      ,\n                      <jats:italic>S. chromogens<\/jats:italic>\n                      ,\n                      <jats:italic>S. cohnii<\/jats:italic>\n                      , and\n                      <jats:italic>S. captis<\/jats:italic>\n                      species were isolated at the rates of 11, 5.2, 3.4, 3.1, 3.1, 1, 1, and 0.7% respectively. All the isolates showed a high percentage (100%) of resistance to Amoxicillin, Ampicillin, and Cefotetan and 37.5% of resistance to Oxacillin. The majority (54.2%) of coagulase-negative isolates also showed multidrug resistance. Coagulase-negative S\n                      <jats:italic>taphylococcus<\/jats:italic>\n                      species carried the\n                      <jats:italic>icaD<\/jats:italic>\n                      ,\n                      <jats:italic>pvl<\/jats:italic>\n                      ,\n                      <jats:italic>mecA<\/jats:italic>\n                      ,\n                      <jats:italic>hlb<\/jats:italic>\n                      ,\n                      <jats:italic>sec<\/jats:italic>\n                      , and\n                      <jats:italic>hla<\/jats:italic>\n                      virulent genes at the rates of 26.5%, 22.1%, 21.7%, 9.6%, 9.6% and 8.4% respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The present study revealed that the majority of the isolates (54.2%) were found multidrug-resistant and carriage of one or more virulent and enterotoxin genes responsible for intramammary and food poisoning infections. Thus, urgent disease control and prevention measures are warranted to reduce the deleterious impact of coagulase-negative species. To the best of our knowledge, this is the first study in Ethiopia to detect coagulase-negative\n                      <jats:italic>Staphylococcus<\/jats:italic>\n                      species with their associated virulent and food poisoning genes from bovine milk.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12866-024-03415-0","type":"journal-article","created":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T06:02:03Z","timestamp":1721282523000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Coagulase-negative staphylococci from bovine milk: Antibiogram profiles and virulent gene detection"],"prefix":"10.1186","volume":"24","author":[{"given":"Yared Abate","family":"Getahun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Solomon Lulie","family":"Abey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Achenef Melaku","family":"Beyene","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mequanint Addisu","family":"Belete","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tesfaye Sisay","family":"Tessema","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,7,18]]},"reference":[{"key":"3415_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2022\/9084334","volume":"2022","author":"F Abunna","year":"2022","unstructured":"Abunna F, Adugna B, Tufa TB, Ayana D, Gutema FD, Waktole H, et al. 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Thus, the University of Gondar sent a letter of cooperation to the study area. Furthermore, an official permission letter was obtained from the livestock and fishery production office to conduct the study. The purpose and procedures of the study were properly explained to the farm owner and study participants and milk samples were collected from dairy farms, whose owners showed a willingness to participate and informed consent was obtained from all of them. All methods were performed in accordance with the relevant guidelines and regulations.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval and consent to Participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"263"}}