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It is an intracellular product of <jats:italic>Streptomyces avermitilis<\/jats:italic>; is synthesized through complicated pathways and can then be extracted from mycelial by methanol extraction. ABA serves as a biological control substance against the root-knot nematode <jats:italic>Meloidogyne incognita<\/jats:italic>. This investigation is intended to reach a new strain of <jats:italic>S. avermitilis<\/jats:italic> capable of producing ABA effectively.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Among the sixty actinobacterial isolates, <jats:italic>Streptomyces<\/jats:italic> St.53 isolate was chosen for its superior nematicidal effectiveness. The mycelial-methanol extract of isolate St.53 exhibited a maximum in vitro mortality of 100% in one day. In the greenhouse experiment, the mycelial-methanol extract demonstrated, for the second-stage juveniles (J<jats:sub>2<\/jats:sub>s), 75.69% nematode reduction and 0.84 reproduction rate (Rr) while for the second-stage juveniles (J<jats:sub>2<\/jats:sub>s), the culture suspension demonstrated 75.38% nematode reduction and 0.80 reproduction rate (Rr). Molecular identification for St.53 was performed using 16\u00a0S rRNA gene analysis and recorded in NCBI Genbank as <jats:italic>S. avermitilis<\/jats:italic> MICNEMA2022 with accession number (<jats:italic>OP108264.1<\/jats:italic>). LC-MS was utilized to detect and identify abamectin in extracts while HPLC analysis was carried out for quantitative determination. Both abamectin B1a and abamectin B1b were produced and detected at retention times of 4.572 and 3.890\u00a0min respectively.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p><jats:italic>Streptomyces avermitilis<\/jats:italic> MICNEMA2022 proved to be an effective source for producing abamectin as a biorational agent for integrated nematode management.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-024-03466-3","type":"journal-article","created":{"date-parts":[[2024,9,7]],"date-time":"2024-09-07T06:02:15Z","timestamp":1725688935000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Streptomyces avermitilis MICNEMA2022: a new biorational strain for producing abamectin as an integrated nematode management agent"],"prefix":"10.1186","volume":"24","author":[{"given":"Wafaa H.","family":"Radwan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed A. 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