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An official permission letter was obtained from Nekemte Public Health Research and Referral Laboratory Center. Participants were informed of the purpose of the study, risks associated with the study, confidentiality of personal data, and their right to take part in the study. After that, we obtained a written informed consent from adult study participants, whereas an assent was obtained from study participants less than 18 years of age. In addition, a consent was also obtained from their parents or legal guardians to participate in this study. Finally, specimens were collected from all study participants and analyzed accordingly. 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