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Its tendency to acquire or donate resistance genes to neighboring bacteria is a major concern. Tetracyclines have shown promise in treating <jats:italic>A. baumannii<\/jats:italic> infections, but tetracycline resistance is growing globally in <jats:italic>A. baumannii<\/jats:italic> isolates.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Objectives<\/jats:title>\n            <jats:p>The study aimed to study (1) the prevalence of multidrug-resistant (MDR) <jats:italic>A. baumannii<\/jats:italic> infections at Suez Canal University Hospitals, (2) the distribution of efflux pump genes <jats:italic>AdeA &amp;B<\/jats:italic>, <jats:italic>TetA<\/jats:italic>, and <jats:italic>TetB<\/jats:italic>, and (3) the effect of efflux pump inhibitor (CCCP) on tetracycline-resistant isolates.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Clinical samples (457) were collected (blood, urine, sputum, ETA, pus, and pleural fluid), followed by <jats:italic>A. baumannii<\/jats:italic> isolation and identification, PCR detection of efflux pump genes, and detection of tetracycline susceptibility and its MIC before and after treatment with the efflux pump inhibitor (CCCP).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>A total of 31 <jats:italic>A. baumannii<\/jats:italic> isolates were recovered (6.78%). The highest rate of isolation was from the ICU (48.3%) from the ET aspirate samples (48.3%). The efflux system AdeA and TetB genes were distributed in 100% of isolates, whereas AdeB was found in 93.5% of isolates and the TetA gene in 87.1% of isolates. All <jats:italic>A. baumannii<\/jats:italic> isolates were MDR showing resistance to three or more classes of antibiotics. 45% of the isolates showed a 4-fold reduction of MIC and 12.9% showed a 2-fold reduction in the MIC.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Efflux pump is an important mechanism for tetracycline resistance among <jats:italic>A. baumannii<\/jats:italic> isolates.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/s12866-024-03735-1","type":"journal-article","created":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T12:41:20Z","timestamp":1738672880000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Detection of AdeAB, TetA, and TetB efflux pump genes in clinical isolates of tetracycline-resistant Acinetobacter baumannii from patients of Suez Canal University Hospitals"],"prefix":"10.1186","volume":"25","author":[{"given":"Hasnaa","family":"Azab","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aya Mohamed","family":"Askar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noha M. 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