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This study aims to investigate some characteristics of the novel Se-NPs\/Cu<jats:sub>2<\/jats:sub>O nanocomposite such as morphological, physicochemical, and optical properties, as well as to assess the antibacterial activity of this fabricated composite in different concentrations against some MDR Gram-positive and Gram-negative clinical bacterial isolates.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>The Se-NPs\/Cu<jats:sub>2<\/jats:sub>O nanocomposite was fabricated using the chemical deposition method. The fabricated nanocomposite was fully characterized by X-Ray diffraction analysis (XRD), fourier transforms infrared spectroscopy (FTIR), and transmission electron microscope (TEM). The antimicrobial activity of Se-NPs\/Cu<jats:sub>2<\/jats:sub>O was investigated using the standard broth microdilution method. The fabricated Se-NPs\/Cu<jats:sub>2<\/jats:sub>O nanocomposites were detected as stable and highly crystallized nanospheres with an average size of 98.6\u00a0nm.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The Se-NPs\/Cu<jats:sub>2<\/jats:sub>O nanocomposite showed a potent antimicrobial activity with MIC values ranged from 6.25 to 12.5\u00a0\u00b5g\/ml for Gram-positive isolates, and 25 to 50\u00a0\u00b5g\/ml for gram-negative isolates. The bactericidal activity was higher for gram-negative isolates with MBC\/MIC ratios of 1\u20132\u00a0\u00b5g\/ml for gram-negative, versus 8\u00a0\u00b5g\/ml for gram positive pathogens.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>These findings would support further research in development of a novel Se-NPs\/Cu<jats:sub>2<\/jats:sub>O nanocomposite as a promising alternative therapeutic option for improving the quality of patients\u2019 management.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/s12866-025-03743-9","type":"journal-article","created":{"date-parts":[[2025,1,20]],"date-time":"2025-01-20T05:38:02Z","timestamp":1737351482000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Antibacterial activity of selenium nanoparticles\/copper oxide (SeNPs\/CuO) nanocomposite against some multi-drug resistant clinical pathogens"],"prefix":"10.1186","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1422-1117","authenticated-orcid":false,"given":"Ahmed Morad","family":"Asaad","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8183-7868","authenticated-orcid":false,"given":"Sara A.","family":"Saied","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad M.","family":"Torayah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. 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