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Hence, assessing new strategies for managing Multi-Drug Resistant (MDR) microorganisms is vital. In this study, the use of mechanically isolated mature adipose cells (MIMACs) and their lysate (Adipolysate) as a new sustainable antimicrobial agent was assessed against Methicillin-resistant <jats:italic>Staphylococcus aureus<\/jats:italic> (MRSA).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>The minimum volume of MIMACs achieved complete bacterial inhibition (Minimum Lethal volume) was 75\u00a0\u00b5l and 100\u00a0\u00b5l for bacterial concentration of 10<jats:sup>10<\/jats:sup> and 10<jats:sup>12<\/jats:sup>\u00a0cfu\/ml, respectively. Direct bacterial membrane attachment and intracellular capture was visualized under light and electron microscopy. Adipolysate was characterized via GC\u2013MS, the fatty acid profile demonstrated several components with known antimicrobial properties. The tested Adipolysate revealed inhibition zone of diameter 25.33\u2009\u00b1\u20090.88\u00a0mm against the tested <jats:italic>S. aureus<\/jats:italic> strain, compared with the inhibition zone of Vancomycin (24.0\u2009\u00b1\u20090.00\u00a0mm) and Erythromycin (30.0\u2009\u00b1\u20090.00). The study revealed the potential effects of MIMACs and Adipolysate as sustainable, natural, and robust antimicrobial agents. However, these preliminary results will be further investigated to understand the mechanism of action and explore possible applications in various fields.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/s12866-025-03747-5","type":"journal-article","created":{"date-parts":[[2025,1,29]],"date-time":"2025-01-29T07:01:17Z","timestamp":1738134077000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Sustainable utilization of bovine adipose tissue derivatives as robust antimicrobial agents against Methicillin-resistant Staphylococcus aureus"],"prefix":"10.1186","volume":"25","author":[{"given":"Muhammed Abdelhameed Ismael","family":"Alcici","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salma Waheed","family":"Abdelhaleem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karima Mogahed","family":"Fahim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neveen Mohamed","family":"Saleh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heba Saeed","family":"Farag","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,1,29]]},"reference":[{"key":"3747_CR1","doi-asserted-by":"crossref","unstructured":"Zhao C, Wang Y, Mulchandani R, Van Boeckel TP. 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