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The aim of this study was to genotypically and phenotypically investigate quinolone resistance of isolates and transferability of <jats:italic>oqxAB<\/jats:italic> genes by conjugation. One hundred <jats:italic>E. coli<\/jats:italic> isolates were collected from UTIs samples and identified using biochemical techniques and confirmed by VITEK-2 System. Antibiotic resistance of isolates was determined by disc diffusion method. MIC of levofloxacin was determined using the broth microdilution method. Efflux pump-mediated resistance was assessed using the chlorpromazine-based efflux-pump inhibitor microplate assay. PMQR genes (<jats:italic>oqxA<\/jats:italic>, <jats:italic>oqxB<\/jats:italic>) were detected by conventional PCR. A conjugation experiment was run to investigate the transferability of the quinolone resistance in having plasmids carrying <jats:italic>oqxAB<\/jats:italic>. DNA sequencing was performed for the identification of genes in both donors and tranconjugants. 80% of <jats:italic>E. coli<\/jats:italic> isolates were resistant for levofloxacin. Chlorpromazine significantly decreased the levofloxacin MIC values. <jats:italic>oqxA<\/jats:italic> and <jats:italic>oqxB<\/jats:italic> genes were detected in 44% and 39% of levofloxacin resistant isolates, respectively. The conjugation experiment revealed the transfer of resistance. MICs of levofloxacin in transconjugants carrying <jats:italic>oqxAB<\/jats:italic> significantly increased as compared to the parental recipients MICs. In conclusion, plasmid-mediated quinolone resistance linked to <jats:italic>oqxAB<\/jats:italic> may be a factor in rapid rise in and spread of bacterial quinolone resistance among Egyptian <jats:italic>E. coli<\/jats:italic> isolates. Chlorpromazine could inhibit efflux pump activity leading to decreased quinolones resistance improving their effectiveness in treatment infectious diseases.<\/jats:p>","DOI":"10.1186\/s12866-025-03850-7","type":"journal-article","created":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T05:17:36Z","timestamp":1742966256000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Chlorpromazine inhibits the plasmid-mediated oqxAB multidrug efflux pump in Escherichia coli isolates of Egyptian patients with utis"],"prefix":"10.1186","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8544-8426","authenticated-orcid":false,"given":"Kholoud","family":"Baraka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2357-7932","authenticated-orcid":false,"given":"Rania","family":"Abozahra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma","family":"Okda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah M.","family":"Abdelhamid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,3,26]]},"reference":[{"issue":"60B","key":"3850_CR1","doi-asserted-by":"crossref","first-page":"3185","DOI":"10.9734\/jpri\/2021\/v33i60B34995","volume":"27","author":"A Jaybhaye","year":"2021","unstructured":"Jaybhaye A, Deb M. 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