{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T07:11:32Z","timestamp":1783062692055,"version":"3.54.6"},"reference-count":48,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T00:00:00Z","timestamp":1746144000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T00:00:00Z","timestamp":1746144000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"Research Center for Global and Local Infectious Diseases","award":["2022B13"],"award-info":[{"award-number":["2022B13"]}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["21K08010"],"award-info":[{"award-number":["21K08010"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["21K07898"],"award-info":[{"award-number":["21K07898"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["18KK0266, 19H03473, 21H00346 and 22H02871"],"award-info":[{"award-number":["18KK0266, 19H03473, 21H00346 and 22H02871"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DK62813"],"award-info":[{"award-number":["DK62813"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["e-ASIA JRP, SATREPS, GACD"],"award-info":[{"award-number":["e-ASIA JRP, SATREPS, GACD"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"DOI":"10.1186\/s12866-025-03990-w","type":"journal-article","created":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T02:23:30Z","timestamp":1746152610000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Identification of genetic determinants of antibiotic resistance in Helicobacter pylori isolates in Vietnam by high-throughput sequencing"],"prefix":"10.1186","volume":"25","author":[{"given":"Phuc Hoang","family":"Bui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trang Ngoc Minh","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanh Truc","family":"Tran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Matsumoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junko","family":"Akada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshio","family":"Yamaoka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,5,2]]},"reference":[{"issue":"11","key":"3990_CR1","doi-asserted-by":"publisher","first-page":"784","DOI":"10.1056\/NEJMoa001999","volume":"345","author":"N Uemura","year":"2001","unstructured":"Uemura N, et al. Helicobacter pylori infection and the development of gastric cancer. N Engl J Med. 2001;345(11):784\u20139.","journal-title":"N Engl J Med"},{"issue":"9","key":"3990_CR2","doi-asserted-by":"publisher","first-page":"1598","DOI":"10.1136\/gutjnl-2019-319696","volume":"69","author":"Y Guo","year":"2020","unstructured":"Guo Y, et al. Effect of Helicobacter pylori on gastrointestinal microbiota: a population-based study in Linqu, a high-risk area of gastric cancer. Gut. 2020;69(9):1598\u2013607.","journal-title":"Gut"},{"issue":"1","key":"3990_CR3","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1016\/S2468-1253(23)00281-9","volume":"9","author":"TC Hong","year":"2024","unstructured":"Hong TC, et al. Primary antibiotic resistance of Helicobacter pylori in the Asia-Pacific region between 1990 and 2022: an updated systematic review and meta-analysis. Lancet Gastroenterol Hepatol. 2024;9(1):56\u201367.","journal-title":"Lancet Gastroenterol Hepatol"},{"issue":"9","key":"3990_CR4","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1038\/s41575-021-00449-x","volume":"18","author":"E Tshibangu-Kabamba","year":"2021","unstructured":"Tshibangu-Kabamba E, Yamaoka Y. Helicobacter pylori infection and antibiotic resistance - from biology to clinical implications. Nat Rev Gastroenterol Hepatol. 2021;18(9):613\u201329.","journal-title":"Nat Rev Gastroenterol Hepatol"},{"key":"3990_CR5","doi-asserted-by":"crossref","unstructured":"Tuan, V.P., et al., A Next-Generation Sequencing-Based Approach to Identify Genetic Determinants of Antibiotic Resistance in Cambodian Helicobacter pylori Clinical Isolates. J Clin Med. 2019;8(6):858.","DOI":"10.3390\/jcm8060858"},{"key":"3990_CR6","doi-asserted-by":"crossref","unstructured":"Tshibangu-Kabamba, E., et al., Next-Generation Sequencing of the Whole Bacterial Genome for Tracking Molecular Insight into the Broad-Spectrum Antimicrobial Resistance of Helicobacter pylori Clinical Isolates from the Democratic Republic of Congo. Microorganisms. 2020;8(6):887.","DOI":"10.3390\/microorganisms8060887"},{"issue":"3","key":"3990_CR7","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00258-21","volume":"35","author":"S Ansari","year":"2022","unstructured":"Ansari S, Yamaoka Y. Helicobacter pylori Infection, Its Laboratory Diagnosis, and Antimicrobial Resistance: a Perspective of Clinical Relevance. Clin Microbiol Rev. 2022;35(3): e0025821.","journal-title":"Clin Microbiol Rev"},{"issue":"5","key":"3990_CR8","doi-asserted-by":"publisher","first-page":"396","DOI":"10.1111\/j.1083-4389.2004.00267.x","volume":"9","author":"S Toracchio","year":"2004","unstructured":"Toracchio S, et al. Identification of a novel mutation affecting domain V of the 23S rRNA gene in Helicobacter pylori. Helicobacter. 2004;9(5):396\u20139.","journal-title":"Helicobacter"},{"issue":"4","key":"3990_CR9","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1111\/j.1523-5378.2006.00415.x","volume":"11","author":"H Miyachi","year":"2006","unstructured":"Miyachi H, et al. Primary levofloxacin resistance and gyrA\/B mutations among Helicobacter pylori in Japan. Helicobacter. 2006;11(4):243\u20139.","journal-title":"Helicobacter"},{"issue":"1","key":"3990_CR10","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1038\/nrg.2016.132","volume":"18","author":"RA Power","year":"2017","unstructured":"Power RA, Parkhill J, de Oliveira T. Microbial genome-wide association studies: lessons from human GWAS. Nat Rev Genet. 2017;18(1):41\u201350.","journal-title":"Nat Rev Genet"},{"key":"3990_CR11","doi-asserted-by":"crossref","unstructured":"Tuan, V.P., et al., Genome-wide association study of gastric cancer- and duodenal ulcer-derived Helicobacter pylori strains reveals discriminatory genetic variations and novel oncoprotein candidates. Microb Genom. 2021;7(11):000680.","DOI":"10.1099\/mgen.0.000680"},{"issue":"1","key":"3990_CR12","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1186\/s12915-018-0550-3","volume":"16","author":"E Berthenet","year":"2018","unstructured":"Berthenet E, et al. A GWAS on Helicobacter pylori strains points to genetic variants associated with gastric cancer risk. BMC Biol. 2018;16(1):84.","journal-title":"BMC Biol"},{"key":"3990_CR13","doi-asserted-by":"crossref","unstructured":"van Vliet, A.H., Use of pan-genome analysis for the identification of lineage-specific genes of Helicobacter pylori. FEMS Microbiol Lett. 2017;364(2):fnw296.","DOI":"10.1093\/femsle\/fnw296"},{"key":"3990_CR14","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1186\/s13099-017-0195-8","volume":"9","author":"TT Binh","year":"2017","unstructured":"Binh TT, et al. Advanced non-cardia gastric cancer and Helicobacter pylori infection in Vietnam. Gut Pathog. 2017;9:46.","journal-title":"Gut Pathog"},{"issue":"15","key":"3990_CR15","doi-asserted-by":"publisher","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","volume":"30","author":"AM Bolger","year":"2014","unstructured":"Bolger AM, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics. 2014;30(15):2114\u201320.","journal-title":"Bioinformatics"},{"issue":"6","key":"3990_CR16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005595","volume":"13","author":"RR Wick","year":"2017","unstructured":"Wick RR, et al. Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput Biol. 2017;13(6): e1005595.","journal-title":"PLoS Comput Biol"},{"issue":"14","key":"3990_CR17","doi-asserted-by":"publisher","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","volume":"30","author":"T Seemann","year":"2014","unstructured":"Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics. 2014;30(14):2068\u20139.","journal-title":"Bioinformatics"},{"issue":"1","key":"3990_CR18","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1186\/s13059-020-02090-4","volume":"21","author":"G Tonkin-Hill","year":"2020","unstructured":"Tonkin-Hill G, et al. Producing polished prokaryotic pangenomes with the Panaroo pipeline. Genome Biol. 2020;21(1):180.","journal-title":"Genome Biol"},{"issue":"5","key":"3990_CR19","doi-asserted-by":"publisher","first-page":"1530","DOI":"10.1093\/molbev\/msaa015","volume":"37","author":"BQ Minh","year":"2020","unstructured":"Minh BQ, et al. IQ-TREE 2: New Models and Efficient Methods for Phylogenetic Inference in the Genomic Era. Mol Biol Evol. 2020;37(5):1530\u20134.","journal-title":"Mol Biol Evol"},{"issue":"2","key":"3990_CR20","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1093\/bioinformatics\/btx610","volume":"34","author":"J Hadfield","year":"2018","unstructured":"Hadfield J, et al. Phandango: an interactive viewer for bacterial population genomics. Bioinformatics. 2018;34(2):292\u20133.","journal-title":"Bioinformatics"},{"key":"3990_CR21","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.4588","volume":"6","author":"M Roosaare","year":"2018","unstructured":"Roosaare M, et al. PlasmidSeeker: identification of known plasmids from bacterial whole genome sequencing reads. PeerJ. 2018;6: e4588.","journal-title":"PeerJ"},{"issue":"10","key":"3990_CR22","volume":"3","author":"M Hunt","year":"2017","unstructured":"Hunt M, et al. ARIBA: rapid antimicrobial resistance genotyping directly from sequencing reads. Microb Genom. 2017;3(10): e000131.","journal-title":"Microb Genom"},{"key":"3990_CR23","doi-asserted-by":"crossref","unstructured":"Saranathan, R., et al., Helicobacter pylori Infections in the Bronx, New York: Surveying Antibiotic Susceptibility and Strain Lineage by Whole-Genome Sequencing. J Clin Microbiol. 2020;58(3):e01591\u201319.","DOI":"10.1128\/JCM.01591-19"},{"issue":"24","key":"3990_CR24","doi-asserted-by":"publisher","first-page":"4310","DOI":"10.1093\/bioinformatics\/bty539","volume":"34","author":"JA Lees","year":"2018","unstructured":"Lees JA, et al. pyseer: a comprehensive tool for microbial pangenome-wide association studies. Bioinformatics. 2018;34(24):4310\u20132.","journal-title":"Bioinformatics"},{"issue":"11","key":"3990_CR25","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1007758","volume":"14","author":"M Jaillard","year":"2018","unstructured":"Jaillard M, et al. A fast and agnostic method for bacterial genome-wide association studies: Bridging the gap between k-mers and genetic events. PLoS Genet. 2018;14(11): e1007758.","journal-title":"PLoS Genet"},{"issue":"23","key":"3990_CR26","doi-asserted-by":"publisher","first-page":"4306","DOI":"10.1111\/febs.12020","volume":"279","author":"M Martinez-Julvez","year":"2012","unstructured":"Martinez-Julvez M, et al. Structure of RdxA\u2013an oxygen-insensitive nitroreductase essential for metronidazole activation in Helicobacter pylori. FEBS J. 2012;279(23):4306\u201317.","journal-title":"FEBS J"},{"issue":"6","key":"3990_CR27","doi-asserted-by":"publisher","first-page":"1480","DOI":"10.1021\/acsinfecdis.9b00127","volume":"6","author":"X Ma","year":"2020","unstructured":"Ma X, et al. Structural and Biological Basis of Small Molecule Inhibition of Escherichia coli LpxD Acyltransferase Essential for Lipopolysaccharide Biosynthesis. ACS Infect Dis. 2020;6(6):1480\u20139.","journal-title":"ACS Infect Dis"},{"key":"3990_CR28","doi-asserted-by":"crossref","unstructured":"Abedeera, S.M., et al., Pseudouridine Synthase RsuA Confers a Survival Advantage to Bacteria under Streptomycin Stress. Antibiotics (Basel). 2023;12(9):1447.","DOI":"10.3390\/antibiotics12091447"},{"issue":"3","key":"3990_CR29","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1080\/1040841X.2017.1418285","volume":"44","author":"Y Gong","year":"2018","unstructured":"Gong Y, Yuan Y. Resistance mechanisms of Helicobacter pylori and its dual target precise therapy. Crit Rev Microbiol. 2018;44(3):371\u201392.","journal-title":"Crit Rev Microbiol"},{"issue":"5","key":"3990_CR30","first-page":"349","volume":"21","author":"Y Hu","year":"2016","unstructured":"Hu Y, et al. Helicobacter pylori and Antibiotic Resistance. A Continuing and Intractable Problem Helicobacter. 2016;21(5):349\u201363.","journal-title":"A Continuing and Intractable Problem Helicobacter"},{"issue":"1","key":"3990_CR31","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1186\/s12866-022-02463-8","volume":"22","author":"TT Tran","year":"2022","unstructured":"Tran TT, et al. Emergence of amoxicillin resistance and identification of novel mutations of the pbp1A gene in Helicobacter pylori in Vietnam. BMC Microbiol. 2022;22(1):41.","journal-title":"BMC Microbiol"},{"issue":"1","key":"3990_CR32","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1186\/s12866-021-02096-3","volume":"21","author":"AM Albasha","year":"2021","unstructured":"Albasha AM, et al. Helicobacter pylori 23S rRNA gene A2142G, A2143G, T2182C, and C2195T mutations associated with clarithromycin resistance detected in Sudanese patients. BMC Microbiol. 2021;21(1):38.","journal-title":"BMC Microbiol"},{"issue":"1","key":"3990_CR33","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1186\/s12941-018-0259-8","volume":"17","author":"J Chen","year":"2018","unstructured":"Chen J, et al. Application of next-generation sequencing to characterize novel mutations in clarithromycin-susceptible Helicobacter pylori strains with A2143G of 23S rRNA gene. Ann Clin Microbiol Antimicrob. 2018;17(1):10.","journal-title":"Ann Clin Microbiol Antimicrob"},{"issue":"1","key":"3990_CR34","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1186\/s12890-020-1172-4","volume":"20","author":"S Kabir","year":"2020","unstructured":"Kabir S, et al. Fluoroquinolone resistance and mutational profile of gyrA in pulmonary MDR tuberculosis patients. BMC Pulm Med. 2020;20(1):138.","journal-title":"BMC Pulm Med"},{"key":"3990_CR35","doi-asserted-by":"publisher","first-page":"1435064","DOI":"10.3389\/fcell.2024.1435064","volume":"12","author":"J Huang","year":"2024","unstructured":"Huang J, et al. Functional determination of site-mutations in rdxA involved in metronidazole resistance of Helicobacter pylori. Front Cell Dev Biol. 2024;12:1435064.","journal-title":"Front Cell Dev Biol"},{"issue":"11","key":"3990_CR36","doi-asserted-by":"publisher","first-page":"3152","DOI":"10.1093\/jac\/dkaa302","volume":"75","author":"S Zhang","year":"2020","unstructured":"Zhang S, et al. Mutations of Helicobacter pylori RdxA are mainly related to the phylogenetic origin of the strain and not to metronidazole resistance. J Antimicrob Chemother. 2020;75(11):3152\u20135.","journal-title":"J Antimicrob Chemother"},{"key":"3990_CR37","doi-asserted-by":"publisher","first-page":"2015","DOI":"10.3389\/fmicb.2016.02015","volume":"7","author":"A Hanafi","year":"2016","unstructured":"Hanafi A, et al. Molecular and Proteomic Analysis of Levofloxacin and Metronidazole Resistant Helicobacter pylori. Front Microbiol. 2016;7:2015.","journal-title":"Front Microbiol"},{"issue":"8","key":"3990_CR38","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004547","volume":"10","author":"C Chewapreecha","year":"2014","unstructured":"Chewapreecha C, et al. Comprehensive identification of single nucleotide polymorphisms associated with beta-lactam resistance within pneumococcal mosaic genes. PLoS Genet. 2014;10(8): e1004547.","journal-title":"PLoS Genet"},{"issue":"5","key":"3990_CR39","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1099\/mic.0.000789","volume":"165","author":"S Coulthurst","year":"2019","unstructured":"Coulthurst S. The Type VI secretion system: a versatile bacterial weapon. Microbiology (Reading). 2019;165(5):503\u201315.","journal-title":"Microbiology (Reading)"},{"issue":"4","key":"3990_CR40","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1111\/mmi.13896","volume":"107","author":"E Grohmann","year":"2018","unstructured":"Grohmann E, et al. Type IV secretion in Gram-negative and Gram-positive bacteria. Mol Microbiol. 2018;107(4):455\u201371.","journal-title":"Mol Microbiol"},{"key":"3990_CR41","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1186\/s13100-018-0109-4","volume":"9","author":"RM Delahay","year":"2018","unstructured":"Delahay RM, Croxall NJ, Stephens AD. Phylogeographic diversity and mosaicism of the Helicobacter pylori tfs integrative and conjugative elements. Mob DNA. 2018;9:5.","journal-title":"Mob DNA"},{"issue":"1","key":"3990_CR42","doi-asserted-by":"publisher","first-page":"4584","DOI":"10.1038\/s41598-021-83862-1","volume":"11","author":"BH Phuc","year":"2021","unstructured":"Phuc BH, et al. Helicobacter pylori type 4 secretion systems as gastroduodenal disease markers. Sci Rep. 2021;11(1):4584.","journal-title":"Sci Rep"},{"key":"3990_CR43","doi-asserted-by":"crossref","unstructured":"Kravtsov, D., D. Gryadunov, and B. Shaskolskiy, Gonococcal Genetic Island in the Global Neisseria gonorrhoeae Population: A Model of Genetic Diversity and Association with Resistance to Antimicrobials. Microorganisms. 2023;11(6):1547.","DOI":"10.3390\/microorganisms11061547"},{"issue":"1","key":"3990_CR44","doi-asserted-by":"publisher","first-page":"518","DOI":"10.1128\/AAC.01597-13","volume":"58","author":"A Beceiro","year":"2014","unstructured":"Beceiro A, et al. Biological cost of different mechanisms of colistin resistance and their impact on virulence in Acinetobacter baumannii. Antimicrob Agents Chemother. 2014;58(1):518\u201326.","journal-title":"Antimicrob Agents Chemother"},{"issue":"12","key":"3990_CR45","doi-asserted-by":"publisher","first-page":"4971","DOI":"10.1128\/AAC.00834-10","volume":"54","author":"JH Moffatt","year":"2010","unstructured":"Moffatt JH, et al. Colistin resistance in Acinetobacter baumannii is mediated by complete loss of lipopolysaccharide production. Antimicrob Agents Chemother. 2010;54(12):4971\u20137.","journal-title":"Antimicrob Agents Chemother"},{"key":"3990_CR46","doi-asserted-by":"crossref","unstructured":"Babosan, A., et al., Nonessential tRNA and rRNA modifications impact the bacterial response to sub-MIC antibiotic stress. Microlife, 2022. 3: p. uqac019.","DOI":"10.1093\/femsml\/uqac019"},{"issue":"1","key":"3990_CR47","doi-asserted-by":"publisher","first-page":"14089","DOI":"10.1038\/s41598-017-14372-2","volume":"7","author":"MA Machuca","year":"2017","unstructured":"Machuca MA, et al. Helicobacter pylori chemoreceptor TlpC mediates chemotaxis to lactate. Sci Rep. 2017;7(1):14089.","journal-title":"Sci Rep"},{"key":"3990_CR48","doi-asserted-by":"crossref","unstructured":"Pichon, M., V. Luzarraga, and C. Burucoa, Mutations Associated to Antibiotic-Resistant Helicobacter pylori: It Is Time to Validate Sequencing Observations. J Clin Med. 2022;11(15):4293.","DOI":"10.3390\/jcm11154293"}],"container-title":["BMC Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12866-025-03990-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12866-025-03990-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12866-025-03990-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T02:23:39Z","timestamp":1746152619000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcmicrobiol.biomedcentral.com\/articles\/10.1186\/s12866-025-03990-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,2]]},"references-count":48,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["3990"],"URL":"https:\/\/doi.org\/10.1186\/s12866-025-03990-w","relation":{},"ISSN":["1471-2180"],"issn-type":[{"value":"1471-2180","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,2]]},"assertion":[{"value":"29 February 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 April 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 May 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The study was conducted according to the guidelines of the Declaration of Helsinki and approved by the Ethics Committee of Oita University. Informed consent was obtained from all subjects involved in the study.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not Applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"264"}}