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While the blood-based <jats:italic>ttr<\/jats:italic> and <jats:italic>Inv A<\/jats:italic> qPCR technique showed 82% and 80% positivity, respectively, with a Ct threshold value of 37 cycles for both techniques. The stool culture method revealed <jats:italic>S. enterica<\/jats:italic> infection in only 32% of cases, while blood culture methods identified <jats:italic>S. enterica<\/jats:italic> in 48% of cases.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>The enriched blood-based <jats:italic>ttr<\/jats:italic> and <jats:italic>Inv A<\/jats:italic> qPCR method is a promising rapid tool for diagnosing typhoid fever, offering high sensitivity, specificity, accuracy and unaffected by antibiotic intake.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/s12866-025-04127-9","type":"journal-article","created":{"date-parts":[[2025,7,5]],"date-time":"2025-07-05T03:47:25Z","timestamp":1751687245000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Validation of stool and blood analysis compared to Inv A and ttr based direct blood qPCR assay as diagnostic tools for typhoid fever"],"prefix":"10.1186","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0588-9761","authenticated-orcid":false,"given":"Shymaa A.","family":"Elaskary","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanem Mohamed","family":"Badawy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doaa S.","family":"Elgendy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amany Mohammed","family":"Abdelmaksoud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasha G.","family":"Mostafa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,5]]},"reference":[{"issue":"1","key":"4127_CR1","doi-asserted-by":"publisher","first-page":"69","DOI":"10.21608\/ejmm.2023.277781","volume":"32","author":"NM Mahmoud","year":"2023","unstructured":"Mahmoud NM, Zaki ME, Abd El Salam M, Farag NA, ElkholRMy. 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