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This pathogen has developed resistance to\n                      <jats:italic>\u03b2-lactam<\/jats:italic>\n                      antibiotics due to the expression of extended-spectrum beta-lactamase (ESBL) genes, leading to current treatment challenges in these patients. Bacteriophages are alternative and effective treatment options against multidrug-resistant (MDR) pathogens. In this study, a lytic bacteriophage was isolated from untreated sewage, tested against multidrug-resistant\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      strains, and evaluated for its therapeutic potential\n                      <jats:italic>in vitro.<\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study presents the microbiological, physicochemical, and genomic characterization of a virulent bacteriophage. The phage was studied by electron microscopy, host range analysis, multiplicity of infection (MOI) determination, adsorption rate measurement, burst size calculation, latent period assessment, stability testing to temperature, chloroform, pH and salt stress, and biofilm removal ability evaluation demonstrated by SEM; the bacteriophage genome was studied by complete genome sequencing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The phage exhibited a broad and highly specific host range for\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      strains. Its stability under stress conditions, including changes in temperature, pH, salt concentration, and exposure to chloroform, was 64.63%, 52.79%, 68.36%, and 98.92%, respectively. The one-step growth curve results demonstrated that the bacteriophage had a latent period of 30\u00a0min and a burst size of approximately 98 plaque-forming units per infected cell (PFU\/cell). Adsorption assays revealed that 92% of isolated phages adsorbed to bacterial cells within 5\u00a0min. Additionally, the bacteriophage showed inhibitory activity against bacterial growth at an MOI 1. The biofilm removal assay demonstrated that the phage eliminated over 93% of the cellular biomass, as confirmed by scanning electron microscopy (SEM). Whole-genome analysis showed that it belongs to the\n                      <jats:italic>Loughboroughvirus<\/jats:italic>\n                      genus. The phage has a linear, double-stranded DNA genome with a length of 55,637\u00a0bp and a GC content of 45.9%. The genome encodes 76 open reading frames (ORFs), including structural proteins, DNA metabolism enzymes, lysis modules, and packaging proteins. No tRNAs, lysogenic genes, or virulence factors were detected in the genome.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Characterization of phage VB_KpM-AEV23 demonstrated its high host specificity against\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      , making it a suitable candidate for phage therapy applications.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12866-025-04243-6","type":"journal-article","created":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T12:33:40Z","timestamp":1771418020000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Isolation, characterization, and genomic analysis of novel bacteriophage AEV23 against multidrug-resistant Klebsiella pneumoniae isolated from Cystic Fibrosis patients"],"prefix":"10.1186","volume":"26","author":[{"given":"Atefe","family":"Jafari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6731-5977","authenticated-orcid":false,"given":"Ameneh","family":"Elikaei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erfan","family":"Gowdini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahya Abdi","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sepideh","family":"Meidaninikjeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,18]]},"reference":[{"issue":"1","key":"4243_CR1","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/j.mib.2011.11.001","volume":"15","author":"M Callaghan","year":"2012","unstructured":"Callaghan M, McClean S. 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