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The treatment of\n                      <jats:italic>C. albicans<\/jats:italic>\n                      -infected diabetic wounds is often problematic due to drug resistance and biofilm formation. Therefore, we studied the impact of \u03b2-carotene on treating diabetic wounds infected with strong biofilm-forming multidrug-resistant (MDR)\n                      <jats:italic>C. albicans<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Our data showed that \u03b2-carotene (200\u00a0\u00b5g\/ml) exhibited potent antifungal activity against the planktonic\n                      <jats:italic>C. albicans<\/jats:italic>\n                      cells, including MDR cells, with inhibition zones of 14 to 34\u00a0mm, which was emphasized by methylene blue staining and growth kinetics analysis. Using polystyrene plates and silicon catheter models, \u03b2-carotene showed a promising antibiofilm action by disrupting the established biofilms of\n                      <jats:italic>C. albicans<\/jats:italic>\n                      by 95% and 75.9%, respectively, which was confirmed by crystal violet staining, dual staining, and fluorescence staining approaches. The antibiofilm pathway of \u03b2-carotene was also examined, and it was found that \u03b2-carotene targeted the first stage of biofilm via reducing the adherence up to 90%, which was linked to downregulation of expression of the hypha-specific gene,\n                      <jats:italic>ALS3<\/jats:italic>\n                      , and blocking action of agglutinin-like protein 3 (Als3) via complexation (-9.8\u00a0kcal\/mol). In addition, \u03b2-carotene disrupted the initiation step by inhibiting the yeast-to-hyphae transition and lowering the viability of sessile cells up to 80%. Furthermore, the maturation step of biofilm was embattled by \u03b2-carotene through a 95.6% reduction in the production of polysaccharide matrix. The in vivo model demonstrated the curative role of \u03b2-carotene in the healing process, which displayed a wound healing ratio of 89%, a lower fungal burden, and better histological features.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Our findings demonstrated the therapeutic potential of \u03b2-carotene for treating diabetic wounds infected with strong biofilm-forming MDR\n                      <jats:italic>C. albicans<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12866-025-04447-w","type":"journal-article","created":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T08:10:34Z","timestamp":1763194234000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The dual antifungal and antibiofilm activities of \u03b2-carotene against multidrug-resistant Candida albicans induce wound healing in a diabetic rat model: an in vitro and in vivo study"],"prefix":"10.1186","volume":"25","author":[{"given":"Khaled B.","family":"Al-Monofy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed A.","family":"Abdelaziz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal M.","family":"Abo-Kamar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lamiaa A.","family":"Al-Madboly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud H.","family":"Farghali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,11,15]]},"reference":[{"issue":"1","key":"4447_CR1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-67232-x","volume":"10","author":"S Mirpour","year":"2020","unstructured":"Mirpour S, Fathollah S, Mansouri P, Larijani B, Ghoranneviss M, Mohajeri Tehrani M, et al. 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