{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T12:12:20Z","timestamp":1772453540892,"version":"3.50.1"},"reference-count":62,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T00:00:00Z","timestamp":1765411200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T00:00:00Z","timestamp":1772409600000},"content-version":"vor","delay-in-days":81,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82260003"],"award-info":[{"award-number":["82260003"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82370018"],"award-info":[{"award-number":["82370018"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82360004"],"award-info":[{"award-number":["82360004"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004761","name":"Natural Science Foundation of Hainan Province","doi-asserted-by":"publisher","award":["822QN324"],"award-info":[{"award-number":["822QN324"]}],"id":[{"id":"10.13039\/501100004761","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Hainan Provincial Natural Science Foundation Youth Fund Project","award":["823QN239"],"award-info":[{"award-number":["823QN239"]}]},{"name":"National Natural Science Foundation Joint Fund for Regional Innovation and Development","award":["U24A20747"],"award-info":[{"award-number":["U24A20747"]}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2305000"],"award-info":[{"award-number":["2022YFC2305000"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Nanhai new star\u201d Medical and Health Talent Platform Project in Hainan Province","award":["NHXX WJW-2023015"],"award-info":[{"award-number":["NHXX WJW-2023015"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"DOI":"10.1186\/s12866-025-04588-y","type":"journal-article","created":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T09:46:55Z","timestamp":1765446415000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phage-mediated control of melioidosis in Southern China: molecular insights and therapeutic potential of N4-like viruses from Hainan"],"prefix":"10.1186","volume":"26","author":[{"given":"Shen","family":"Tian","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaxin","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dai","family":"Kuang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meiping","family":"lv","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiao","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiling","family":"Yi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianfeng","family":"Xia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanshuang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,12,11]]},"reference":[{"issue":"1","key":"4588_CR1","doi-asserted-by":"publisher","first-page":"17107","DOI":"10.1038\/nrdp.2017.107","volume":"4","author":"WJ Wiersinga","year":"2018","unstructured":"Wiersinga WJ, Virk HS, Torres AG, Currie BJ, Peacock SJ, Dance DAB, Limmathurotsakul D, Melioidosis. Nat Rev Dis Primers. 2018;4(1):17107.","journal-title":"Nat Rev Dis Primers"},{"issue":"8","key":"4588_CR2","doi-asserted-by":"publisher","first-page":"e334","DOI":"10.1016\/S2542-5196(18)30172-4","volume":"2","author":"PL Bulterys","year":"2018","unstructured":"Bulterys PL, Bulterys MA, Phommasone K, Luangraj M, Mayxay M, Kloprogge S, et al. Climatic drivers of melioidosis in Laos and cambodia: a 16-year case series analysis. Lancet Planet Health. 2018;2(8):e334\u201343.","journal-title":"Lancet Planet Health"},{"key":"4588_CR3","doi-asserted-by":"publisher","first-page":"100004","DOI":"10.1016\/j.lansea.2022.03.004","volume":"2","author":"PR Mohapatra","year":"2022","unstructured":"Mohapatra PR, Mishra B. Burden of melioidosis in India and South asia: challenges and ways forward. Lancet Reg Health Southeast Asia. 2022;2:100004.","journal-title":"Lancet Reg Health Southeast Asia"},{"issue":"4","key":"4588_CR4","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1038\/nrmicro1385","volume":"4","author":"WJ Wiersinga","year":"2006","unstructured":"Wiersinga WJ, van der Poll T, White NJ, Day NP, Peacock SJ. Melioidosis: insights into the pathogenicity of Burkholderia Pseudomallei. Nat Rev Microbiol. 2006;4(4):272\u201382.","journal-title":"Nat Rev Microbiol"},{"issue":"2","key":"4588_CR5","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1128\/CMR.18.2.383-416.2005","volume":"18","author":"AC Cheng","year":"2005","unstructured":"Cheng AC, Currie BJ. Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev. 2005;18(2):383\u2013416.","journal-title":"Clin Microbiol Rev"},{"key":"4588_CR6","doi-asserted-by":"crossref","unstructured":"Zheng X, Xia Q, Xia L, Li W. Endemic melioidosis in Southern china: past and present. Trop Med Infect Dis. 2019;4(1).","DOI":"10.3390\/tropicalmed4010039"},{"key":"4588_CR7","doi-asserted-by":"crossref","unstructured":"Limmathurotsakul D, Golding N, Dance DA, Messina JP, Pigott DM, Moyes CL et al. Predicted global distribution of Burkholderia Pseudomallei and burden of melioidosis. Nat Microbiol. 2016;1(1).","DOI":"10.1038\/nmicrobiol.2015.8"},{"issue":"1","key":"4588_CR8","doi-asserted-by":"publisher","first-page":"100008","DOI":"10.1016\/j.cmicom.2024.100008","volume":"1","author":"EM Meumann","year":"2024","unstructured":"Meumann EM, Currie BJ. Approach to melioidosis. CMI Comms. 2024;1(1):100008.","journal-title":"CMI Comms"},{"issue":"25","key":"4588_CR9","doi-asserted-by":"publisher","first-page":"2355","DOI":"10.1056\/NEJMoa2306448","volume":"389","author":"JK Petras","year":"2023","unstructured":"Petras JK, Elrod MG, Ty MC, Dawson P, O\u2019Laughlin K, Gee JE, et al. Locally acquired melioidosis linked to Environment \u2014 Mississippi, 2020\u20132023. N Engl J Med. 2023;389(25):2355\u201362.","journal-title":"N Engl J Med"},{"issue":"47","key":"4588_CR10","doi-asserted-by":"publisher","first-page":"1055","DOI":"10.46234\/ccdcw2022.212","volume":"4","author":"Y Sun","year":"2022","unstructured":"Sun Y, Yang M, Yu S, Liu J, Li W, Tian J, et al. Emergence of autochthonous melioidosis in two inland PLADs \u2014 China, 2021. China CDC Wkly. 2022;4(47):1055\u20138.","journal-title":"China CDC Wkly"},{"issue":"1","key":"4588_CR11","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1186\/s12879-019-3793-x","volume":"19","author":"N Shrestha","year":"2019","unstructured":"Shrestha N, Adhikari M, Pant V, Baral S, Shrestha A, Basnyat B, et al. Melioidosis: misdiagnosed in Nepal. BMC Infect Dis. 2019;19(1):176.","journal-title":"BMC Infect Dis"},{"issue":"12","key":"4588_CR12","doi-asserted-by":"publisher","first-page":"1389","DOI":"10.2217\/fmb.12.116","volume":"7","author":"HP Schweizer","year":"2012","unstructured":"Schweizer HP. Mechanisms of antibiotic resistance in Burkholderia pseudomallei: implications for treatment of melioidosis. Future Microbiol. 2012;7(12):1389\u201399.","journal-title":"Future Microbiol"},{"key":"4588_CR13","doi-asserted-by":"crossref","unstructured":"Madden DE, Webb JR, Steinig EJ, Currie BJ, Price EP, Sarovich DS. Taking the next-gen step: comprehensive antimicrobial resistance detection from Burkholderia Pseudomallei. EBioMedicine 2021;63.","DOI":"10.1016\/j.ebiom.2020.103152"},{"issue":"9919","key":"4588_CR14","doi-asserted-by":"publisher","first-page":"762","DOI":"10.1016\/S0140-6736(13)62143-1","volume":"383","author":"DA Fisher","year":"2014","unstructured":"Fisher DA, Harris PNA. Melioidosis: refining management of a tropical time bomb. Lancet. 2014;383(9919):762\u20134.","journal-title":"Lancet"},{"key":"4588_CR15","doi-asserted-by":"crossref","unstructured":"Sullivan RP, Marshall CS, Anstey NM, Ward L, Currie BJ. 2020 Review and revision of the 2015 Darwin melioidosis treatment guideline; paradigm drift not shift. PLoS Negl Trop Dis. 2020;14(9):e0008659.","DOI":"10.1371\/journal.pntd.0008659"},{"issue":"4","key":"4588_CR16","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1016\/j.ijantimicag.2014.01.005","volume":"43","author":"D Dance","year":"2014","unstructured":"Dance D. Treatment and prophylaxis of melioidosis. Int J Antimicrob Agents. 2014;43(4):310\u20138.","journal-title":"Int J Antimicrob Agents"},{"issue":"5","key":"4588_CR17","doi-asserted-by":"publisher","first-page":"582","DOI":"10.1016\/j.ijantimicag.2019.01.003","volume":"53","author":"S Chirakul","year":"2019","unstructured":"Chirakul S, Somprasong N, Norris MH, Wuthiekanun V, Chantratita N, Tuanyok A, Schweizer HP. Burkholderia Pseudomallei acquired Ceftazidime resistance due to gene duplication and amplification. Int J Antimicrob Agents. 2019;53(5):582\u20138.","journal-title":"Int J Antimicrob Agents"},{"issue":"9","key":"4588_CR18","doi-asserted-by":"publisher","first-page":"e14461","DOI":"10.1097\/MD.0000000000014461","volume":"98","author":"C Rao","year":"2019","unstructured":"Rao C, Hu Z, Chen J, Tang M, Chen H, Lu X, et al. Molecular epidemiology and antibiotic resistance of Burkholderia Pseudomallei isolates from Hainan, china: A STROBE compliant observational study. Med (Baltim). 2019;98(9):e14461.","journal-title":"Med (Baltim)"},{"issue":"1","key":"4588_CR19","doi-asserted-by":"publisher","first-page":"10652","DOI":"10.1038\/s41598-018-28843-7","volume":"8","author":"S Chirakul","year":"2018","unstructured":"Chirakul S, Norris MH, Pagdepanichkit S, Somprasong N, Randall LB, Shirley JF, et al. Transcriptional and post-transcriptional regulation of PenA \u03b2-lactamase in acquired Burkholderia Pseudomallei \u03b2-lactam resistance. Sci Rep. 2018;8(1):10652.","journal-title":"Sci Rep"},{"key":"4588_CR20","doi-asserted-by":"publisher","first-page":"101121","DOI":"10.1016\/j.drup.2024.101121","volume":"76","author":"S Tian","year":"2024","unstructured":"Tian S, Wu X, Liu L, Li A, Li X, Pei H, et al. Point mutation P174L of the PenA gene endowing Ceftazidime resistance to Burkholderia Pseudomallei in China. Drug Resist Updat. 2024;76:101121.","journal-title":"Drug Resist Updat"},{"issue":"1","key":"4588_CR21","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1186\/s40249-022-01012-9","volume":"11","author":"Y Wang","year":"2022","unstructured":"Wang Y, Li X, Dance DAB, Xia H, Chen C, Luo N, et al. A novel lytic phage potentially effective for phage therapy against Burkholderia Pseudomallei in the tropics. Infect Dis Poverty. 2022;11(1):87.","journal-title":"Infect Dis Poverty"},{"key":"4588_CR22","doi-asserted-by":"crossref","unstructured":"Kang X, Fu Z, Rajaofera MJN, Li C, Zhang N, Liu L et al. Whole-Genome sequence of Burkholderia Pseudomallei strain HNBP001, isolated from a melioidosis patient in Hainan, China. Microbiol Resour Announc. 2019;8(36).","DOI":"10.1128\/MRA.00471-19"},{"issue":"W1","key":"4588_CR23","doi-asserted-by":"publisher","first-page":"W16","DOI":"10.1093\/nar\/gkw387","volume":"44","author":"D Arndt","year":"2016","unstructured":"Arndt D, Grant JR, Marcu A, Sajed T, Pon A, Liang Y, Wishart DS. PHASTER: a better, faster version of the PHAST phage search tool. Nucleic Acids Res. 2016;44(W1):W16\u201321.","journal-title":"Nucleic Acids Res"},{"key":"4588_CR24","unstructured":"Andrews S. FastQC: a quality control tool for high throughput sequence data. 2010."},{"key":"4588_CR25","doi-asserted-by":"crossref","unstructured":"Bolger AM, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics. 2014;30(15):2114\u201320.","DOI":"10.1093\/bioinformatics\/btu170"},{"issue":"5","key":"4588_CR26","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1089\/cmb.2012.0021","volume":"19","author":"A Bankevich","year":"2012","unstructured":"Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol. 2012;19(5):455\u201377.","journal-title":"J Comput Biol"},{"issue":"Web Server issu","key":"4588_CR27","doi-asserted-by":"publisher","first-page":"W451","DOI":"10.1093\/nar\/gki487","volume":"33","author":"J Besemer","year":"2005","unstructured":"Besemer J, Borodovsky M. GeneMark: web software for gene finding in prokaryotes, eukaryotes and viruses. Nucleic Acids Res. 2005;33(Web Server issue):W451\u20134.","journal-title":"Nucleic Acids Res"},{"issue":"W1","key":"4588_CR28","doi-asserted-by":"publisher","first-page":"W54","DOI":"10.1093\/nar\/gkw413","volume":"44","author":"TM Lowe","year":"2016","unstructured":"Lowe TM, Chan PP. tRNAscan-SE On-line: integrating search and context for analysis of transfer RNA genes. Nucleic Acids Res. 2016;44(W1):W54\u20137.","journal-title":"Nucleic Acids Res"},{"issue":"3","key":"4588_CR29","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","volume":"215","author":"SF Altschul","year":"1990","unstructured":"Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990;215(3):403\u201310.","journal-title":"J Mol Biol"},{"issue":"Web Server issu","key":"4588_CR30","doi-asserted-by":"publisher","first-page":"W181","DOI":"10.1093\/nar\/gkn179","volume":"36","author":"JR Grant","year":"2008","unstructured":"Grant JR, Stothard P. The CGView server: a comparative genomics tool for circular genomes. Nucleic Acids Res. 2008;36(Web Server issue):W181\u20134.","journal-title":"Nucleic Acids Res"},{"issue":"10","key":"4588_CR31","doi-asserted-by":"publisher","first-page":"1281","DOI":"10.1007\/s10482-017-0844-4","volume":"110","author":"SH Yoon","year":"2017","unstructured":"Yoon SH, Ha SM, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie Van Leeuwenhoek. 2017;110(10):1281\u20136.","journal-title":"Antonie Van Leeuwenhoek"},{"key":"4588_CR32","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1007\/978-1-62703-646-7_12","volume":"1079","author":"B Morgenstern","year":"2014","unstructured":"Morgenstern B. Multiple sequence alignment with DIALIGN. Methods Mol Biol. 2014;1079:191\u2013202.","journal-title":"Methods Mol Biol"},{"key":"4588_CR33","doi-asserted-by":"crossref","unstructured":"Kumar S, Stecher G, Suleski M, Sanderford M, Sharma S, Tamura K. MEGA12: molecular evolutionary genetic analysis version 12 for adaptive and green computing. Mol Biol Evol 2024;41(12).","DOI":"10.1093\/molbev\/msae263"},{"issue":"15","key":"4588_CR34","doi-asserted-by":"publisher","first-page":"2379","DOI":"10.1093\/bioinformatics\/btx157","volume":"33","author":"Y Nishimura","year":"2017","unstructured":"Nishimura Y, Yoshida T, Kuronishi M, Uehara H, Ogata H, Goto S. ViPTree: the viral proteomic tree server. Bioinformatics. 2017;33(15):2379\u201380.","journal-title":"Bioinformatics"},{"issue":"8","key":"4588_CR35","doi-asserted-by":"publisher","first-page":"e0153624","DOI":"10.1128\/mbio.01536-24","volume":"15","author":"B Aldeguer-Riquelme","year":"2024","unstructured":"Aldeguer-Riquelme B, Conrad RE, Ant\u00f3n J, Rossello-Mora R, Konstantinidis KT. A natural ANI gap that can define intra-species units of bacteriophages and other viruses. mBio. 2024;15(8):e0153624.","journal-title":"mBio"},{"issue":"46","key":"4588_CR36","doi-asserted-by":"publisher","first-page":"e2406771121","DOI":"10.1073\/pnas.2406771121","volume":"121","author":"Y Zhang","year":"2024","unstructured":"Zhang Y, McWhorter KL, Rosen PC, Klaus JR, Gallant \u00c9, Amaya Lopez CY, et al. Combatting melioidosis with chemical synthetic lethality. Proc Natl Acad Sci U S A. 2024;121(46):e2406771121.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"4588_CR37","doi-asserted-by":"crossref","unstructured":"Ross BN, Myers JN, Muruato LA, Tapia D, Torres AG. Evaluating new compounds to treat Burkholderia Pseudomallei infections. Front Cell Infect Microbiol. 2018;8\u20132018.","DOI":"10.3389\/fcimb.2018.00210"},{"key":"4588_CR38","doi-asserted-by":"crossref","unstructured":"Egido JE, Costa AR, Aparicio-Maldonado C, Haas P-J, Brouns SJJ. Mechanisms and clinical importance of bacteriophage resistance. FEMS Microbiol Rev. 2021;46(1).","DOI":"10.1093\/femsre\/fuab048"},{"issue":"9","key":"4588_CR39","doi-asserted-by":"publisher","first-page":"e0005005","DOI":"10.1371\/journal.pntd.0005005","volume":"10","author":"P Withatanung","year":"2016","unstructured":"Withatanung P, Chantratita N, Muangsombut V, Saiprom N, Lertmemongkolchai G, Klumpp J, et al. Analyses of the distribution patterns of Burkholderia Pseudomallei and associated phages in soil samples in Thailand suggest that phage presence reduces the frequency of bacterial isolation. PLoS Negl Trop Dis. 2016;10(9):e0005005.","journal-title":"PLoS Negl Trop Dis"},{"key":"4588_CR40","doi-asserted-by":"crossref","unstructured":"Czajkowski R, Poluri KM. Bacteriophages isolation from the environment and their antimicrobial therapeutic potential. Volume 2. Frontiers Media SA; 2022.","DOI":"10.3389\/978-2-88974-533-3"},{"issue":"1","key":"4588_CR41","doi-asserted-by":"publisher","first-page":"372","DOI":"10.1038\/s41467-021-27583-z","volume":"13","author":"KM Kauffman","year":"2022","unstructured":"Kauffman KM, Chang WK, Brown JM, Hussain FA, Yang J, Polz MF, Kelly L. Resolving the structure of phage\u2013bacteria interactions in the context of natural diversity. Nat Commun. 2022;13(1):372.","journal-title":"Nat Commun"},{"issue":"21","key":"4588_CR42","doi-asserted-by":"publisher","first-page":"5901","DOI":"10.1016\/j.cell.2024.09.009","volume":"187","author":"M Koncz","year":"2024","unstructured":"Koncz M, Stirling T, Hadj Mehdi H, M\u00e9hi O, Eszenyi B, Asb\u00f3th A, et al. Genomic surveillance as a scalable framework for precision phage therapy against antibiotic-resistant pathogens. Cell. 2024;187(21):5901\u2013e1828.","journal-title":"Cell"},{"key":"4588_CR43","doi-asserted-by":"crossref","unstructured":"Rohde C, Resch G, Pirnay JP, Blasdel BG, Debarbieux L, Gelman D et al. Expert opinion on three phage therapy related topics: bacterial phage Resistance, phage training and prophages in bacterial production strains. Viruses. 2018;10(4).","DOI":"10.3390\/v10040178"},{"issue":"11","key":"4588_CR44","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1007\/s00705-024-06143-y","volume":"169","author":"P Simmonds","year":"2024","unstructured":"Simmonds P, Adriaenssens EM, Lefkowitz EJ, Oksanen HM, Siddell SG, Zerbini FM, et al. Changes to virus taxonomy and the ICTV statutes ratified by the international committee on taxonomy of viruses (2024). Arch Virol. 2024;169(11):236.","journal-title":"Arch Virol"},{"key":"4588_CR45","volume-title":"One-step phage growth assay (Stukey lab) \u2013 protocol v170526. SEA-PHAGES protocol collection","author":"L Stukey","year":"2017","unstructured":"Stukey L. One-step phage growth assay (Stukey lab) \u2013 protocol v170526. SEA-PHAGES protocol collection. USA: SEA-PHAGES; 2017."},{"key":"4588_CR46","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1186\/1471-2180-11-181","volume":"11","author":"R Gallet","year":"2011","unstructured":"Gallet R, Kannoly S, Wang IN. Effects of bacteriophage traits on plaque formation. BMC Microbiol. 2011;11:181.","journal-title":"BMC Microbiol"},{"issue":"1","key":"4588_CR47","doi-asserted-by":"publisher","first-page":"4288","DOI":"10.1038\/s41598-022-07763-7","volume":"12","author":"AV Letarov","year":"2022","unstructured":"Letarov AV, Letarova MA, Ivanov PA, Belalov IS, Clokie MRJ, Galyov EE. Genetic analysis of the cold-sensitive growth phenotype of Burkholderia pseudomallei\/thailandensis bacteriophage AMP1. Sci Rep. 2022;12(1):4288.","journal-title":"Sci Rep"},{"issue":"10","key":"4588_CR48","doi-asserted-by":"publisher","first-page":"e1012525","DOI":"10.1371\/journal.pcbi.1012525","volume":"20","author":"Y Zhang","year":"2024","unstructured":"Zhang Y, Mao M, Zhang R, Liao Y-T, Wu VCH. DeepPL: A deep-learning-based tool for the prediction of bacteriophage lifecycle. PLoS Comput Biol. 2024;20(10):e1012525.","journal-title":"PLoS Comput Biol"},{"issue":"17","key":"4588_CR49","doi-asserted-by":"publisher","first-page":"7033","DOI":"10.1073\/pnas.0610627104","volume":"104","author":"EK Davydova","year":"2007","unstructured":"Davydova EK, Santangelo TJ, Rothman-Denes LB. Bacteriophage N4 virion RNA polymerase interaction with its promoter DNA hairpin. Proc Natl Acad Sci U S A. 2007;104(17):7033\u20138.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"3","key":"4588_CR50","doi-asserted-by":"publisher","first-page":"726","DOI":"10.1016\/j.jmb.2008.02.059","volume":"378","author":"KH Choi","year":"2008","unstructured":"Choi KH, McPartland J, Kaganman I, Bowman VD, Rothman-Denes LB, Rossmann MG. Insight into DNA and protein transport in double-stranded DNA viruses: the structure of bacteriophage N4. J Mol Biol. 2008;378(3):726\u201336.","journal-title":"J Mol Biol"},{"issue":"5","key":"4588_CR51","doi-asserted-by":"publisher","first-page":"e00197","DOI":"10.1128\/msystems.00197-23","volume":"8","author":"K Zheng","year":"2023","unstructured":"Zheng K, Liang Y, Paez-Espino D, Zou X, Gao C, Shao H, et al. Identification of hidden N4-like viruses and their interactions with hosts. mSystems. 2023;8(5):e00197\u201323.","journal-title":"mSystems"},{"key":"4588_CR52","doi-asserted-by":"crossref","unstructured":"Wittmann J, Turner D, Millard AD, Mahadevan P, Kropinski AM, Adriaenssens EM. From orphan phage to a proposed new Family-the diversity of N4-Like viruses. Antibiot (Basel). 2020;9(10).","DOI":"10.3390\/antibiotics9100663"},{"key":"4588_CR53","volume-title":"Bacteriophage N4 comparative genomics and the mechanism of N4 RNAPII","author":"BR Lenneman","year":"2019","unstructured":"Lenneman BR. Bacteriophage N4 comparative genomics and the mechanism of N4 RNAPII. Transcription Initiation: University of Chicago; 2019."},{"issue":"6","key":"4588_CR54","doi-asserted-by":"publisher","first-page":"e03246","DOI":"10.1128\/mbio.03246-21","volume":"12","author":"B Sellner","year":"2021","unstructured":"Sellner B, Prakapait\u0117 R, Berkum Mv, Heinemann M, Harms A, Jenal U. A new sugar for an old phage: a c-di-GMP-Dependent polysaccharide pathway sensitizes Escherichia coli for bacteriophage infection. mBio. 2021;12(6):e03246\u201321.","journal-title":"mBio"},{"issue":"6","key":"4588_CR55","doi-asserted-by":"publisher","first-page":"1329","DOI":"10.3390\/v14061329","volume":"14","author":"D Boeckaerts","year":"2022","unstructured":"Boeckaerts D, Stock M, De Baets B, Briers Y. Identification of phage Receptor-Binding protein sequences with hidden Markov models and an extreme gradient boosting classifier. Viruses. 2022;14(6):1329.","journal-title":"Viruses"},{"issue":"1","key":"4588_CR56","doi-asserted-by":"publisher","first-page":"2273567","DOI":"10.1080\/21505594.2023.2273567","volume":"14","author":"Z Guo","year":"2023","unstructured":"Guo Z, Liu M, Zhang D. Potential of phage depolymerase for the treatment of bacterial biofilms. Virulence. 2023;14(1):2273567.","journal-title":"Virulence"},{"issue":"5","key":"4588_CR57","doi-asserted-by":"publisher","first-page":"e05304","DOI":"10.1128\/spectrum.05304-22","volume":"11","author":"R Cai","year":"2023","unstructured":"Cai R, Ren Z, Zhao R, Lu Y, Wang X, Guo Z, et al. Structural biology and functional features of phage-derived depolymerase Depo32 on Klebsiella pneumoniae Ith K2 serotype capsular polysaccharides. Microbiol Spectr. 2023;11(5):e05304\u201322.","journal-title":"Microbiol Spectr"},{"issue":"3","key":"4588_CR58","doi-asserted-by":"publisher","first-page":"1209","DOI":"10.1128\/IAI.05805-11","volume":"80","author":"J Cuccui","year":"2012","unstructured":"Cuccui J, Milne TS, Harmer N, George AJ, Harding SV, Dean RE, et al. Characterization of the Burkholderia Pseudomallei K96243 capsular polysaccharide I coding region. Infect Immun. 2012;80(3):1209\u201321.","journal-title":"Infect Immun"},{"key":"4588_CR59","doi-asserted-by":"publisher","first-page":"465890","DOI":"10.1016\/j.chroma.2025.465890","volume":"1749","author":"JPP Saavedra","year":"2025","unstructured":"Saavedra JPP, Silva-Santos AR, Duarte SOD, Azevedo AM. Scalable purification of bacteriophages preparations. J Chromatogr A. 2025;1749:465890.","journal-title":"J Chromatogr A"},{"issue":"7","key":"4588_CR60","doi-asserted-by":"publisher","first-page":"e0158213","DOI":"10.1371\/journal.pone.0158213","volume":"11","author":"O Guang-Han","year":"2016","unstructured":"Guang-Han O, Leang-Chung C, Vellasamy KM, Mariappan V, Li-Yen C, Vadivelu J. Experimental phage therapy for Burkholderia Pseudomallei infection. PLoS ONE. 2016;11(7):e0158213.","journal-title":"PLoS ONE"},{"issue":"1","key":"4588_CR61","doi-asserted-by":"publisher","first-page":"e01359","DOI":"10.1128\/jvi.01359-23","volume":"98","author":"X Tan","year":"2024","unstructured":"Tan X, Chen K, Jiang Z, Liu Z, Wang S, Ying Y, et al. Evaluation of the impact of repeated intravenous phage doses on mammalian host\u2013phage interactions. J Virol. 2024;98(1):e01359\u201323.","journal-title":"J Virol"},{"issue":"1","key":"4588_CR62","doi-asserted-by":"publisher","first-page":"759","DOI":"10.1038\/s41598-024-51316-z","volume":"14","author":"M Gallorini","year":"2024","unstructured":"Gallorini M, Marinacci B, Pellegrini B, Cataldi A, Dindo ML, Carradori S, Grande R. Immunophenotyping of hemocytes from infected Galleria Mellonella larvae as an innovative tool for immune profiling, infection studies and drug screening. Sci Rep. 2024;14(1):759.","journal-title":"Sci Rep"}],"container-title":["BMC Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12866-025-04588-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12866-025-04588-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12866-025-04588-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T11:45:28Z","timestamp":1772451928000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1186\/s12866-025-04588-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,11]]},"references-count":62,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["4588"],"URL":"https:\/\/doi.org\/10.1186\/s12866-025-04588-y","relation":{},"ISSN":["1471-2180"],"issn-type":[{"value":"1471-2180","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,11]]},"assertion":[{"value":"26 July 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 November 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 December 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Ethics approval: Not applicable. Experiments involving\n                      G. mellonella\n                      larvae are not subject to ethical approval under Chinese legislation. Larvae were euthanized by freezing at \u2212\u200920\u00a0\u00b0C for 24\u00a0h, a humane method for invertebrates.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"189"}}