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This study analyzed mycobacterial culture and identification data from Henan Provincial Chest Hospital (2020\u20132024) to assess NTM detection patterns and species distribution.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This retrospective laboratory surveillance study analyzed mycobacterial culture and species identification data from Henan Provincial Chest Hospital (January 2020\u2013December 2024). Cultures were performed using the MGIT system with MPB64 antigen detection for MTBC and PNB growth for presumptive NTM identification. Species identification employed DNA microarray targeting the 16\u201323\u00a0S rRNA internal transcribed spacer region. Clinical data (symptoms, radiology, comorbidities), repeated cultures per ATS guidelines, and treatment outcomes were not available. GeneXpert MTB\/RIF-negative cases with cycle threshold less than 42 from 2022 to 2024 were analyzed retrospectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Among 6,939 culture-positive specimens, 962 (13.86%) were identified as NTM, with annual isolation rates increasing from 10.76% to 16.37%. Of these 962 NTM cases, 532 isolates (55.3%) underwent DNA microarray species identification, revealing\n                      <jats:italic>M. intracellulare<\/jats:italic>\n                      as predominant (79.51%, 423\/532), followed by\n                      <jats:italic>M. abscessus<\/jats:italic>\n                      complex (10.53%, 56\/532),\n                      <jats:italic>M. kansasii<\/jats:italic>\n                      (4.32%, 23\/532),\n                      <jats:italic>M. avium<\/jats:italic>\n                      (3.01%, 16\/532), and\n                      <jats:italic>M. fortuitum<\/jats:italic>\n                      (1.69%, 9\/532). Among 532 NTM isolates undergoing species identification, males (59.77%) and elderly patients (&gt;\u200960 years) predominated. In a subgroup of 230 patients with GeneXpert MTB\/RIF-negative results but cycle threshold (Ct) values\u2009&lt;\u200942, 101 were identified as NTM-positive. Notably, 94.25% of these cases were attributed to the\n                      <jats:italic>Mycobacterium avium<\/jats:italic>\n                      complex (MAC), with 93 showing low Ct for probe A and 7 for probe C.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      NTM isolation rates are rising in Henan Province, with\n                      <jats:italic>M. intracellulare<\/jats:italic>\n                      as the predominant species. GeneXpert-negative cases with low Ct values for probe A may benefit from supplementary acid-fast staining and species identification to enhance early NTM detection, findings represent laboratory detection patterns from single specimens and cannot distinguish colonization from clinically significant disease per ATS\/IDSA diagnostic criteria.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12866-026-04736-y","type":"journal-article","created":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T03:15:43Z","timestamp":1768792543000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Analysis of nontuberculous mycobacteria laboratory detection data from a designated hospital in Henan Province, China"],"prefix":"10.1186","volume":"26","author":[{"given":"Jiao","family":"Tan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honghua","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yachun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Zang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingcai","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,1,19]]},"reference":[{"issue":"4","key":"4736_CR1","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1164\/rccm.200604-571ST","volume":"175","author":"DE Griffith","year":"2007","unstructured":"Griffith DE, Aksamit T, Brown-Elliott BA, Catanzaro A, Daley C, Gordin F, et al. 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