{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:12:29Z","timestamp":1787238749998,"version":"build-2736575974"},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T00:00:00Z","timestamp":1771891200000},"content-version":"vor","delay-in-days":28,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"Shaanxi Province key research and development plan for general projects","award":["2023-YBSF"],"award-info":[{"award-number":["2023-YBSF"]}]},{"name":"Shaanxi Province key research and development plan for general projects","award":["2023-YBSF"],"award-info":[{"award-number":["2023-YBSF"]}]},{"name":"Shaanxi Province key research and development plan for general projects","award":["2023-YBSF"],"award-info":[{"award-number":["2023-YBSF"]}]},{"name":"Shaanxi Province key research and development plan for general projects","award":["2023-YBSF"],"award-info":[{"award-number":["2023-YBSF"]}]},{"name":"Shaanxi Province Innovation Capability Support Plan Innovative talent Promotion Plan-Science and technology innovation team","award":["S2022-ZC-TD-0143"],"award-info":[{"award-number":["S2022-ZC-TD-0143"]}]},{"name":"Shaanxi Province Innovation Capability Support Plan Innovative talent Promotion Plan-Science and technology innovation team","award":["S2022-ZC-TD-0143"],"award-info":[{"award-number":["S2022-ZC-TD-0143"]}]},{"name":"Shaanxi Province Innovation Capability Support Plan Innovative talent Promotion Plan-Science and technology innovation team","award":["S2022-ZC-TD-0143"],"award-info":[{"award-number":["S2022-ZC-TD-0143"]}]},{"name":"Shaanxi Province Innovation Capability Support Plan Innovative talent Promotion Plan-Science and technology innovation team","award":["S2022-ZC-TD-0143"],"award-info":[{"award-number":["S2022-ZC-TD-0143"]}]},{"name":"Shaanxi Provincial People's Hospital Support Program of Science and Technology Talents","award":["2021JY-16"],"award-info":[{"award-number":["2021JY-16"]}]},{"name":"Shaanxi Provincial People's Hospital Support Program of Science and Technology Talents","award":["2021JY-16"],"award-info":[{"award-number":["2021JY-16"]}]},{"name":"Shaanxi Provincial People's Hospital Support Program of Science and Technology Talents","award":["2021JY-16"],"award-info":[{"award-number":["2021JY-16"]}]},{"name":"Shaanxi Provincial People's Hospital Support Program of Science and Technology Talents","award":["2021JY-16"],"award-info":[{"award-number":["2021JY-16"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"DOI":"10.1186\/s12866-026-04740-2","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T07:13:34Z","timestamp":1769498014000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Transcriptomics combined with metabolomics to elucidate the mechanisms of bacterial otitis media infection: insights from mitochondrial dysfunction"],"prefix":"10.1186","volume":"26","author":[{"given":"Qimei","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,1,27]]},"reference":[{"key":"4740_CR1","doi-asserted-by":"publisher","first-page":"764772","DOI":"10.3389\/fcimb.2021.764772","volume":"11","author":"HM Massa","year":"2021","unstructured":"Massa HM, Spann KM, Cripps AW. Innate immunity in the middle ear mucosa. Front Cell Infect Microbiol. 2021;11:764772.","journal-title":"Front Cell Infect Microbiol"},{"issue":"Suppl 1","key":"4740_CR2","doi-asserted-by":"publisher","first-page":"S9","DOI":"10.1016\/S0264-410X(00)00272-3","volume":"19","author":"R Dagan","year":"2000","unstructured":"Dagan R. Treatment of acute otitis media - challenges in the era of antibiotic resistance. Vaccine. 2000;19(Suppl 1):S9\u201316.","journal-title":"Vaccine"},{"issue":"1","key":"4740_CR3","first-page":"53","volume":"36","author":"AA Mohamed Yusoff","year":"2024","unstructured":"Mohamed Yusoff AA, Mohd Khair SZN. Unraveling mitochondrial dysfunction: comprehensive perspectives on its impact on neurodegenerative diseases. Nat Rev Neurosci. 2024;36(1):53\u201390.","journal-title":"Nat Rev Neurosci"},{"issue":"17","key":"4740_CR4","doi-asserted-by":"publisher","first-page":"13033","DOI":"10.3390\/ijms241713033","volume":"24","author":"H Grel","year":"2023","unstructured":"Grel H, Woznica D, Ratajczak K, Kalwarczyk E, Anchimowicz J, Switlik W, Olejnik P, Zielonka P, Stobiecka M, Jakiela S. Mitochondrial dynamics in neurodegenerative diseases: unraveling the role of fusion and fission processes. Int J Mol Sci. 2023;24(17):13033.","journal-title":"Int J Mol Sci"},{"issue":"1","key":"4740_CR5","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1186\/s40478-025-01964-7","volume":"13","author":"K Blair","year":"2025","unstructured":"Blair K, Martinez-Serra R, Gosset P, Mart\u00edn-Guerrero SM, M\u00f3rotz GM, Atherton J, Mitchell JC, Markovinovic A, Miller CCJ. Structural and functional studies of the VAPB-PTPIP51 ER-mitochondria tethering proteins in neurodegenerative diseases. Acta Neuropathol Commun. 2025;13(1):49.","journal-title":"Acta Neuropathol Commun"},{"issue":"6","key":"4740_CR6","doi-asserted-by":"publisher","first-page":"C1248","DOI":"10.1152\/ajpcell.00035.2022","volume":"322","author":"A Georgiev","year":"2022","unstructured":"Georgiev A, Granata C, Roden M. The role of mitochondria in the pathophysiology and treatment of common metabolic diseases in humans. Am J Physiol Cell Physiol. 2022;322(6):C1248\u201359.","journal-title":"Am J Physiol Cell Physiol"},{"key":"4740_CR7","doi-asserted-by":"publisher","first-page":"102946","DOI":"10.1016\/j.redox.2023.102946","volume":"68","author":"L Yao","year":"2023","unstructured":"Yao L, Liang X, Liu Y, Li B, Hong M, Wang X, Chen B, Liu Z, Wang P. Non-steroidal mineralocorticoid receptor antagonist finerenone ameliorates mitochondrial dysfunction via PI3K\/Akt\/eNOS signaling pathway in diabetic tubulopathy. Redox Biol. 2023;68:102946.","journal-title":"Redox Biol"},{"issue":"23","key":"4740_CR8","doi-asserted-by":"publisher","first-page":"17124","DOI":"10.3390\/ijms242317124","volume":"24","author":"MJ S\u00e1nchez-Quintero","year":"2023","unstructured":"S\u00e1nchez-Quintero MJ, Rodr\u00edguez-D\u00edaz C, Rodr\u00edguez-Gonz\u00e1lez FJ, Fern\u00e1ndez-Casta\u00f1er A, Garc\u00eda-Fuentes E, L\u00f3pez-G\u00f3mez C. Role of mitochondria in inflammatory bowel diseases: A systematic review. Int J Mol Sci. 2023;24(23):17124.","journal-title":"Int J Mol Sci"},{"key":"4740_CR9","first-page":"1","volume":"18","author":"S Amjad","year":"2023","unstructured":"Amjad S, Ghazaei C. Advances in Understanding the pathogenesis: bacterial surface glycans as the virulence factor and role in pathogen bacteria. ASM Sci J. 2023;18:1\u201313.","journal-title":"ASM Sci J"},{"issue":"6","key":"4740_CR10","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1007\/s12264-022-00993-9","volume":"39","author":"F Li","year":"2022","unstructured":"Li F, Ke H, Wang S, Mao W, Fu C, Chen X, Fu Q, Qin X, Huang Y, Li B, et al. Leaky gut plays a critical role in the pathophysiology of autism in mice by activating the Lipopolysaccharide-Mediated Toll-Like receptor 4\u2013Myeloid differentiation factor 88\u2013Nuclear factor kappa B signaling pathway. Neurosci Bull. 2022;39(6):911\u201328.","journal-title":"Neurosci Bull"},{"key":"4740_CR11","unstructured":"Huang GX, Hallen NR, Lee M, Zheng K, Wang X, Mandanas MV, Djeddi S, Fernandez D, Hacker J, Ryan T. : 2023."},{"key":"4740_CR12","unstructured":"Bacigalupa ZA, Brown WA, Krystofiak ES, Wolf MM, Hongo RA, Landis M, Amason EK, Beckermann KE, Rathmell JC. Rathmell WK: 2022."},{"key":"4740_CR13","doi-asserted-by":"crossref","unstructured":"Houston R, Sekine S, Calderon MJ, Seifuddin F, Wang G, Kawagishi H, Malide DA, Li Y, Gucek M, Pirooznia M, et al. Acetylation-mediated remodeling of the nucleolus regulates cellular acetyl-CoA responses. PLoS Biol. 2020;18(11):e3000981.","DOI":"10.1371\/journal.pbio.3000981"},{"issue":"23","key":"4740_CR14","doi-asserted-by":"publisher","first-page":"4155","DOI":"10.1242\/jcs.011163","volume":"120","author":"Y Chen","year":"2007","unstructured":"Chen Y, McMillan-Ward E, Kong J, Israels SJ, Gibson SB. Mitochondrial electron-transport-chain inhibitors of complexes I and II induce autophagic cell death mediated by reactive oxygen species. J Cell Sci. 2007;120(23):4155\u201366.","journal-title":"J Cell Sci"},{"key":"4740_CR15","doi-asserted-by":"crossref","unstructured":"Scheid AD, Beadnell TC, Welch DR. The second genome: effects of the mitochondrial genome on cancer progression. In.; 2019: 63\u2013105.","DOI":"10.1016\/bs.acr.2019.01.001"},{"key":"4740_CR16","doi-asserted-by":"crossref","unstructured":"Giuffr\u00e8 A, Schimo S, Wittig I, Pos KM, Ludwig B. Cytochrome c oxidase biogenesis and metallochaperone interactions: steps in the assembly pathway of a bacterial complex. PLoS ONE. 2017;12(1):e0170037.","DOI":"10.1371\/journal.pone.0170037"},{"issue":"4","key":"4740_CR17","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1002\/humu.1380050404","volume":"5","author":"M Tuchman","year":"1995","unstructured":"Tuchman M, Plante RJ. Mutations and polymorphisms in the human ornithine transcarbamylase gene: mutation update addendum. Hum Mutat. 1995;5(4):293\u20135.","journal-title":"Hum Mutat"},{"key":"4740_CR18","doi-asserted-by":"crossref","unstructured":"Caron-Godon CA, Collington E, Wolf JL, Coletta G, Glerum DM. More than just bread and wine: using yeast to understand inherited cytochrome oxidase deficiencies in humans. Int J Mol Sci. 2024;25(7):3814.","DOI":"10.3390\/ijms25073814"},{"key":"4740_CR19","unstructured":"Brischigliaro M, Cunatova K, Cabrera-Orefice A, Pei J, Franchin C, Roverso M, Povea-Cabello S, Bogialli S, Arrigoni G, Arnold S. 2024."},{"issue":"7399","key":"4740_CR20","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1038\/nature11007","volume":"485","author":"U F\u00fcnfschilling","year":"2012","unstructured":"F\u00fcnfschilling U, Supplie LM, Mahad D, Boretius S, Saab AS, Edgar J, Brinkmann BG, Kassmann CM, Tzvetanova ID, M\u00f6bius W, et al. Glycolytic oligodendrocytes maintain Myelin and long-term axonal integrity. Nature. 2012;485(7399):517\u201321.","journal-title":"Nature"},{"key":"4740_CR21","doi-asserted-by":"crossref","unstructured":"Deng W, Chen Y, Zhang J, Ling J, Xu Z, Zhu Z, Tang X, Liu X, Zhang D, Zhu H et al. Mild therapeutic hypothermia upregulates the O-GlcNAcylation level of COX10 to alleviate mitochondrial damage induced by myocardial ischemia\u2013reperfusion injury. J Translational Med. 2024;22(1):489.","DOI":"10.1186\/s12967-024-05264-x"},{"issue":"34","key":"4740_CR22","doi-asserted-by":"publisher","first-page":"35334","DOI":"10.1074\/jbc.M404747200","volume":"279","author":"Y-C Horng","year":"2004","unstructured":"Horng Y-C, Cobine PA, Maxfield AB, Carr HS, Winge DR. Specific copper transfer from the Cox17 metallochaperone to both Sco1 and Cox11 in the assembly of yeast cytochrome c oxidase. J Biol Chem. 2004;279(34):35334\u201340.","journal-title":"J Biol Chem"},{"issue":"4","key":"4740_CR23","doi-asserted-by":"publisher","first-page":"181","DOI":"10.14712\/fb2019065040181","volume":"65","author":"M Vani\u0161ov\u00e1","year":"2019","unstructured":"Vani\u0161ov\u00e1 M, Bursk\u00e1 D, K\u0159\u00ed\u017eov\u00e1 J, Da\u0148helovsk\u00e1 T, Dosoudilov\u00e1 \u017d, Zeman J, Stib\u016frek L, Hans\u00edkov\u00e1 H. Stable COX17 downregulation leads to alterations in mitochondrial Ultrastructure, decreased copper content and impaired cytochrome c oxidase biogenesis in HEK293 cells. Folia Biol (Praha). 2019;65(4):181\u20137.","journal-title":"Folia Biol (Praha)"},{"issue":"24","key":"4740_CR24","doi-asserted-by":"publisher","first-page":"15304","DOI":"10.1074\/jbc.M115.645069","volume":"290","author":"M Chojnacka","year":"2015","unstructured":"Chojnacka M, Gornicka A, Oeljeklaus S, Warscheid B, Chacinska A. Cox17 protein is an auxiliary factor involved in the control of the mitochondrial contact site and Cristae organizing system. J Biol Chem. 2015;290(24):15304\u201312.","journal-title":"J Biol Chem"},{"issue":"7","key":"4740_CR25","doi-asserted-by":"publisher","first-page":"5072","DOI":"10.1074\/jbc.M311772200","volume":"279","author":"AB Maxfield","year":"2004","unstructured":"Maxfield AB, Heaton DN, Winge DR. Cox17 is functional when tethered to the mitochondrial inner membrane. J Biol Chem. 2004;279(7):5072\u201380.","journal-title":"J Biol Chem"},{"issue":"34","key":"4740_CR26","doi-asserted-by":"publisher","first-page":"20531","DOI":"10.1074\/jbc.271.34.20531","volume":"271","author":"DM Glerum","year":"1996","unstructured":"Glerum DM, Shtanko A, Tzagoloff A. SCO1 and SCO2 act as high copy suppressors of a mitochondrial copper recruitment defect in Saccharomyces cerevisiae. J Biol Chem. 1996;271(34):20531\u20135.","journal-title":"J Biol Chem"}],"container-title":["BMC Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12866-026-04740-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12866-026-04740-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12866-026-04740-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T10:23:54Z","timestamp":1771928634000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1186\/s12866-026-04740-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,27]]},"references-count":26,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["4740"],"URL":"https:\/\/doi.org\/10.1186\/s12866-026-04740-2","relation":{},"ISSN":["1471-2180"],"issn-type":[{"value":"1471-2180","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,27]]},"assertion":[{"value":"18 May 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 January 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 January 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"166"}}