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However, the genetic underpinnings of its virulence and antimicrobial resistance remain poorly characterized. Leveraging our previously assembled collection of 197\n                      <jats:italic>E. anophelis<\/jats:italic>\n                      isolates with complete genome sequences, we performed an exhaustive, large-scale comparative analysis across global datasets to systematically map resistance determinants and virulence factors. Additionally, we constructed an integrated coexpression network to elucidate genotype-phenotype correlations in antibiotic resistance patterns.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The distribution of virulence-associated genes displays moderate phylogenetic specificity, exhibiting constrained variation within established primary clades. Importantly, virulence gene profiles demonstrate little discernible association with specimen source, sample category, or geographic origin. Although diverse antimicrobial resistance genotypes were detected, these exhibited negligible lineage specificity with minimal differentiation among core phylogenetic clusters. Coexpression network analysis suggests that\n                      <jats:italic>E. anophelis<\/jats:italic>\n                      resistance to cephalosporins, carbapenems, and aztreonam primarily arises from the constitutive expression of chromosomally encoded resistance determinants. In contrast, these chromosomally encoded resistance mechanisms appear to exert either negligible or clinically insignificant effects on susceptibility patterns for the remaining fourteen tested antimicrobial agents.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study demonstrates that the multidrug resistance and pathogenic potential of\n                      <jats:italic>E. anophelis<\/jats:italic>\n                      are predominantly intrinsic traits, regulated by complex, multilayered biological mechanisms. Comprehensive elucidation of the bacterium\u2019s virulence and resistance pathways necessitates the discovery of novel molecular targets followed by systematic, in-depth characterization.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12866-026-04846-7","type":"journal-article","created":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T04:57:04Z","timestamp":1772168224000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genomic perspectives on the global dissemination of Elizabethkingia anophelis: unveiling inherent multidrug resistance and virulence determinants"],"prefix":"10.1186","volume":"26","author":[{"given":"Shaohua","family":"Hu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohua","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujun","family":"Ni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonghong","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beiwen","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,27]]},"reference":[{"issue":"4","key":"4846_CR1","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1016\/j.jhin.2014.01.009","volume":"86","author":"SS Jean","year":"2014","unstructured":"Jean SS, Lee WS, Chen FL, Ou TY, Hsueh PR. 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