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However, it is very rare for HvKp to be cultured from bone marrow aspirates. In this study, four K1-ST700\n                      <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                      (\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      ) isolates were acquired from the bone marrow, blood, liver abscess puncture fluid, and bronchoalveolar lavage fluid (BALF) of a 53-year-old male patient in a Chinese hospital. The patient rapidly developed fatal multiple-organ failure caused by K1-ST700 HvKp infection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Isolates were determined as\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      via the VITEK-2 Compact system and validated by the VITEK-MS system. Antimicrobial susceptibility assessment of four isolates was conducted via the VITEK-2 Compact system. MLST of\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      was conducted following the Pasteur Institute protocol. The virulence of isolates was detected through serotype and mouse lethality assay. The bone marrow isolate (KPN-BM) underwent whole-genome sequencing and was compared to available K1\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      genomes from China.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      All isolates exhibited high virulence in a mouse infection model and remained susceptible to all tested antibiotics. The complete genome sequence was deposited in GenBank (accession no. JBRNXP000000000). A total of 133 virulence-associated genes were identified in KPN-BM, including genes involved in iron acquisition, immune evasion, bacterial adherence, and type VI secretion. A total of 64\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      isolates of K1 reported in China exhibited stable phylogenetic relationships. Fifty-eight strains belong to the ST23 type. Five K1-ST700\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      strains from China and Norway carry similar virulence genes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>K1-ST700 HvKp may represent an emerging high virulence clone with the potential for transmission. HvKp-induced bone marrow infection is very rare and should be given clinical attention.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12866-026-05068-7","type":"journal-article","created":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T10:55:37Z","timestamp":1781002537000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phenotype and molecular characterization of K1-ST700 hypervirulent Klebsiella pneumoniae isolated from bone marrow aspirate of a patient with multi-system infection"],"prefix":"10.1186","volume":"26","author":[{"given":"Chunhong","family":"Shao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingchao","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meijie","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,9]]},"reference":[{"issue":"23","key":"5068_CR1","doi-asserted-by":"publisher","first-page":"3027","DOI":"10.1016\/j.scib.2023.10.038","volume":"68","author":"L Liu","year":"2023","unstructured":"Liu L, Lou N, Liang Q, Xiao W, Teng G, Ma J, et al. 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Ethical approval was granted by the Ethics Committee of Taian City Central Hospital affiliated with Qingdao University (Shandong, China), which waived the requirement for informed consent given the retrospective, observational nature of the research and the use of anonymized patient data.Animal studies were reviewed and approved by the Taian City Central Hospital affiliated with Qingdao University Animal Management Committee (Approval Number: 2025-08-D40), ensuring compliance with the Guidelines for the Ethical Review of Laboratory Animal Welfare (People\u2019s Republic of China National Standard GB\/T 35892\u20132018). To ensure a humane endpoint, mice were anesthetized by isoflurane inhalation. Death was verified by cessation of respiration and cardiac activity. 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