{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T19:42:46Z","timestamp":1785526966303,"version":"3.56.0"},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":62,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"2024 Discipline Development Funding - Discipline Development - Laboratory Medicine","award":["202509-5576"],"award-info":[{"award-number":["202509-5576"]}]},{"name":"Research on a Rapid Drug Susceptibility Detection Method for Mycobacterium tuberculosis Based on Single-Cell Raman Technology","award":["FKLY20014"],"award-info":[{"award-number":["FKLY20014"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"DOI":"10.1186\/s12866-026-05151-z","type":"journal-article","created":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T09:34:50Z","timestamp":1780047290000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic clusteringof KPC-2-producing carbapenem-resistant Klebsiella pneumoniae ST5422-KL19 in Shanghai, China"],"prefix":"10.1186","volume":"26","author":[{"given":"Yinjuan","family":"Guo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongpeng","family":"Shang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihua","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haojin","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingjie","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangyou","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,29]]},"reference":[{"issue":"2","key":"5151_CR1","first-page":"176","volume":"69","author":"WP Herridge","year":"2020","unstructured":"Herridge WP, Shibu P, O\u2019Shea J, Brook TC, Hoyles L. Bacteriophages of Klebsiella spp., their diversity and potential therapeutic uses. J Med Microbiol. 2020;69(2):176\u201394.","journal-title":"J Med Microbiol"},{"issue":"6","key":"5151_CR2","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1038\/s41579-019-0315-1","volume":"18","author":"KL Wyres","year":"2020","unstructured":"Wyres KL, Lam MMC, Holt KE. Population genomics of Klebsiella pneumoniae. Nat Rev Microbiol. 2020;18(6):344\u201359.","journal-title":"Nat Rev Microbiol"},{"key":"5151_CR3","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2024.101124","volume":"77","author":"C Liu","year":"2024","unstructured":"Liu C, Guo J, Fan S, Guo W, Qi H, Baker S, et al. An increased prevalence of carbapenem-resistant hypervirulent Klebsiella pneumoniae associated with the COVID-19 pandemic. Drug Resist Updat. 2024;77:101124.","journal-title":"Drug Resist Updat"},{"key":"5151_CR4","doi-asserted-by":"publisher","unstructured":"Harada S, Aoki K, Yamamoto S, Ishii Y, Sekiya N, Kurai H, Furukawa K, Doi A, Tochitani K, Kubo K et al: Clinical and Molecular Characteristics of Klebsiella pneumoniae Isolates Causing Bloodstream Infections in Japan: Occurrence of Hypervirulent Infections in Health Care. J Clin Microbiol. 2019;57(11). https:\/\/doi.org\/10.1128\/jcm.01206-19.","DOI":"10.1128\/jcm.01206-19"},{"issue":"2","key":"5151_CR5","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1093\/cid\/ciac791","volume":"76","author":"CL Luterbach","year":"2023","unstructured":"Luterbach CL, Chen L, Komarow L, Ostrowsky B, Kaye KS, Hanson B, et al. Transmission of carbapenem-resistant Klebsiella pneumoniae in US hospitals. Clin Infect Dis. 2023;76(2):229\u201337.","journal-title":"Clin Infect Dis"},{"key":"5151_CR6","doi-asserted-by":"publisher","unstructured":"Guo Y, Ding L, Han R, Yin D, Wu S, Yang Y, Wang F, Zhu D, Hu F: Antimicrobial resistance profile of clinical isolates from hospitals across China: CHINET 2024 surveillance report. One Health Advances. 2025;3(1). https:\/\/doi.org\/10.1186\/s44280-025-00092-0.","DOI":"10.1186\/s44280-025-00092-0"},{"issue":"2","key":"5151_CR7","doi-asserted-by":"publisher","first-page":"289","DOI":"10.3201\/eid2602.190594","volume":"26","author":"K Zhou","year":"2020","unstructured":"Zhou K, Xiao T, David S, Wang Q, Zhou Y, Guo L, et al. Novel subclone of carbapenem-resistant Klebsiella pneumoniae sequence type 11 with enhanced virulence and transmissibility, China. Emerg Infect Dis. 2020;26(2):289\u201397.","journal-title":"Emerg Infect Dis"},{"key":"5151_CR8","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1016\/j.jgar.2020.09.004","volume":"23","author":"W Liao","year":"2020","unstructured":"Liao W, Liu Y, Zhang W. Virulence evolution, molecular mechanisms of resistance and prevalence of ST11 carbapenem-resistant Klebsiella pneumoniae in China: a review over the last 10 years. J Glob Antimicrob Resist. 2020;23:174\u201380.","journal-title":"J Glob Antimicrob Resist"},{"key":"5151_CR9","doi-asserted-by":"publisher","unstructured":"Weingarten RA, Johnson RC, Conlan S, Ramsburg AM, Dekker JP, Lau AF, Khil P, Odom RT, Deming C, Park M et al: Genomic Analysis of Hospital Plumbing Reveals Diverse Reservoir of Bacterial Plasmids Conferring Carbapenem Resistance. mBio. 2018;9(1). https:\/\/doi.org\/10.1128\/mbio.02011-17.","DOI":"10.1128\/mbio.02011-17"},{"issue":"1","key":"5151_CR10","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/S1473-3099(17)30489-9","volume":"18","author":"D Gu","year":"2018","unstructured":"Gu D, Dong N, Zheng Z, Lin D, Huang M, Wang L, et al. A fatal outbreak of ST11 carbapenem-resistant hypervirulent Klebsiella pneumoniae in a Chinese hospital: a molecular epidemiological study. Lancet Infect Dis. 2018;18(1):37\u201346.","journal-title":"Lancet Infect Dis"},{"issue":"3","key":"5151_CR11","doi-asserted-by":"publisher","first-page":"814","DOI":"10.1038\/s41564-024-01612-1","volume":"9","author":"F Hu","year":"2024","unstructured":"Hu F, Pan Y, Li H, Han R, Liu X, Ma R, et al. Carbapenem-resistant Klebsiella pneumoniae capsular types, antibiotic resistance and virulence factors in China: a longitudinal, multi-centre study. Nat Microbiol. 2024;9(3):814\u201329.","journal-title":"Nat Microbiol"},{"issue":"1","key":"5151_CR12","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-024-10012-z","volume":"24","author":"A Sharma","year":"2024","unstructured":"Sharma A, Vats N, Rati R, Sharma L, Chatterjee N, Jaggi N. Genotypic diversity and virulence factors of Klebsiella pneumoniae in a North Indian tertiary care hospital. BMC Infect Dis. 2024;24(1):1477.","journal-title":"BMC Infect Dis"},{"key":"5151_CR13","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2023.100918","volume":"67","author":"Y Zhou","year":"2023","unstructured":"Zhou Y, Wu C, Wang B, Xu Y, Zhao H, Guo Y, et al. Characterization difference of typical KL1, KL2 and ST11-KL64 hypervirulent and carbapenem-resistant Klebsiella pneumoniae. Drug Resist Updat. 2023;67:100918.","journal-title":"Drug Resist Updat"},{"issue":"1","key":"5151_CR14","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1186\/s12941-023-00588-w","volume":"22","author":"L Yang","year":"2023","unstructured":"Yang L, Zhang G, Zhao Q, Guo L, Yang J. Molecular characteristics of clinical IMP-producing Klebsiella pneumoniae isolates: novel IMP-90 and integron In2147. Ann Clin Microbiol Antimicrob. 2023;22(1):38.","journal-title":"Ann Clin Microbiol Antimicrob"},{"key":"5151_CR15","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1016\/j.jgar.2025.05.004","volume":"43","author":"L Li","year":"2025","unstructured":"Li L, Yi C, Wen Z, Cai X, Jin P. Study on the correlation between carbapenem-resistant Klebsiella pneumoniae infection strains and intestinal colonization strains in intensive care unit of a tertiary hospital in China. J Glob Antimicrob Resist. 2025;43:327\u201336.","journal-title":"J Glob Antimicrob Resist"},{"key":"5151_CR16","unstructured":"Performance Standards for Antimicrobial Susceptibility Testing 35st edn, CLSI supplement M100 (Clinical and Laboratory Standards Institute). 2025."},{"issue":"1","key":"5151_CR17","doi-asserted-by":"publisher","DOI":"10.1002\/cpbi.102","volume":"70","author":"A Prjibelski","year":"2020","unstructured":"Prjibelski A, Antipov D, Meleshko D, Lapidus A, Korobeynikov A. Using SPAdes de novo assembler. Curr Protoc Bioinformatics. 2020;70(1):e102.","journal-title":"Curr Protoc Bioinformatics"},{"issue":"14","key":"5151_CR18","doi-asserted-by":"publisher","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","volume":"30","author":"T Seemann","year":"2014","unstructured":"Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics. 2014;30(14):2068\u20139.","journal-title":"Bioinformatics"},{"key":"5151_CR19","unstructured":"H W: ggplot2: Elegant Graphics for Data Analysis. . Springer 2016."},{"issue":"1","key":"5151_CR20","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1111\/2041-210X.12628","volume":"8","author":"G Yu","year":"2016","unstructured":"Yu G, Smith DK, Zhu H, Guan Y, Lam TTY, McInerny G. Ggtree: an R package for visualization and annotation of phylogenetic trees with their covariates and other associated data. Methods Ecol Evol. 2016;8(1):28\u201336.","journal-title":"Methods Ecol Evol"},{"issue":"2","key":"5151_CR21","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1093\/bioinformatics\/btg412","volume":"20","author":"E Paradis","year":"2004","unstructured":"Paradis E, Claude J, Strimmer K. APE: analyses of phylogenetics and evolution in R language. Bioinformatics. 2004;20(2):289\u201390.","journal-title":"Bioinformatics"},{"issue":"4","key":"5151_CR22","doi-asserted-by":"publisher","first-page":"592","DOI":"10.1093\/bioinformatics\/btq706","volume":"27","author":"KP Schliep","year":"2011","unstructured":"Schliep KP. Phangorn: phylogenetic analysis in R. Bioinformatics. 2011;27(4):592\u20133.","journal-title":"Bioinformatics"},{"issue":"12","key":"5151_CR23","doi-asserted-by":"publisher","first-page":"1053","DOI":"10.1093\/qjmed\/hcu124","volume":"107","author":"A Kumabe","year":"2014","unstructured":"Kumabe A, Kenzaka T. String test of hypervirulent Klebsiella pneumonia. QJM. 2014;107(12):1053.","journal-title":"QJM"},{"issue":"1","key":"5151_CR24","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-024-03614-9","volume":"24","author":"C Wu","year":"2024","unstructured":"Wu C, Huang Y, Zhou P, Gao H, Wang B, Zhao H, et al. Emergence of hypervirulent and carbapenem-resistant Klebsiella pneumoniae from 2014 - 2021 in Central and Eastern China: a molecular, biological, and epidemiological study. BMC Microbiol. 2024;24(1):465.","journal-title":"BMC Microbiol"},{"key":"5151_CR25","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1007\/978-1-4939-9601-8_10","volume":"2021","author":"SD Himpsl","year":"2019","unstructured":"Himpsl SD, Mobley HLT. Siderophore detection using Chrome Azurol S and cross-feeding assays. Methods Mol Biol. 2019;2021:97\u2013108.","journal-title":"Methods Mol Biol"},{"key":"5151_CR26","doi-asserted-by":"publisher","unstructured":"Palacios M, Miner TA, Frederick DR, Sepulveda VE, Quinn JD, Walker KA, Miller VL: Identification of Two Regulators of Virulence That Are Conserved in Klebsiella pneumoniae Classical and Hypervirulent Strains. mBio. 2018;9(4). https:\/\/doi.org\/10.1128\/mbio.01443-18.","DOI":"10.1128\/mbio.01443-18"},{"key":"5151_CR27","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1016\/j.jgar.2023.11.008","volume":"36","author":"S Sun","year":"2024","unstructured":"Sun S, Cai M, Wang Q, Wang S, Zhang L, Wang H. Emergency of the plasmid co-carrying bla(KPC-2) and bla(NDM-1) genes in carbapenem-resistant hypervirulent Klebsiella pneumoniae. J Glob Antimicrob Resist. 2024;36:26\u201332.","journal-title":"J Glob Antimicrob Resist"},{"issue":"2","key":"5151_CR28","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2024.107245","volume":"64","author":"S Wang","year":"2024","unstructured":"Wang S, Ma S, Sun S, Wang Q, Ding Q, Jin L, et al. Global evolutionary dynamics of virulence genes in ST11-KL47 carbapenem-resistant Klebsiella pneumoniae. Int J Antimicrob Agents. 2024;64(2):107245.","journal-title":"Int J Antimicrob Agents"},{"issue":"5","key":"5151_CR29","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1016\/j.ijantimicag.2017.09.011","volume":"50","author":"Y Taniguchi","year":"2017","unstructured":"Taniguchi Y, Maeyama Y, Ohsaki Y, Hayashi W, Osaka S, Koide S, et al. Co-resistance to colistin and tigecycline by disrupting mgrB and ramR with IS insertions in a canine Klebsiella pneumoniae ST37 isolate producing SHV-12, DHA-1 and FosA3. Int J Antimicrob Agents. 2017;50(5):697\u20138.","journal-title":"Int J Antimicrob Agents"},{"issue":"23","key":"5151_CR30","doi-asserted-by":"publisher","first-page":"716","DOI":"10.21037\/atm.2019.12.01","volume":"7","author":"B Gu","year":"2019","unstructured":"Gu B, Bi R, Cao X, Qian H, Hu R, Ma P. Clonal dissemination of KPC-2-producing Klebsiella pneumoniae ST11 and ST48 clone among multiple departments in a tertiary teaching hospital in Jiangsu Province, China. Ann Transl Med. 2019;7(23):716.","journal-title":"Ann Transl Med"},{"key":"5151_CR31","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02178-20","author":"M Wan","year":"2021","unstructured":"Wan M, Gao X, Lv L, Cai Z, Liu JH. IS26 mediates the acquisition of tigecycline resistance gene cluster tmexCD1-toprJ1 by IncHI1B-FIB plasmids in Klebsiella pneumoniae and Klebsiella quasipneumoniae from food market sewage. Antimicrob Agents Chemother. 2021. https:\/\/doi.org\/10.1128\/AAC.02178-20.","journal-title":"Antimicrob Agents Chemother"},{"issue":"11","key":"5151_CR32","doi-asserted-by":"publisher","first-page":"6982","DOI":"10.1128\/AAC.03808-14","volume":"58","author":"ZK Sheng","year":"2014","unstructured":"Sheng ZK, Hu F, Wang W, Guo Q, Chen Z, Xu X, et al. Mechanisms of tigecycline resistance among Klebsiella pneumoniae clinical isolates. Antimicrob Agents Chemother. 2014;58(11):6982\u20135.","journal-title":"Antimicrob Agents Chemother"},{"issue":"Suppl_4","key":"5151_CR33","doi-asserted-by":"publisher","first-page":"S325","DOI":"10.1093\/cid\/ciab784","volume":"73","author":"S Argimon","year":"2021","unstructured":"Argimon S, David S, Underwood A, Abrudan M, Wheeler NE, Kekre M, et al. Rapid genomic characterization and global surveillance of Klebsiella using Pathogenwatch. Clin Infect Dis. 2021;73(Suppl_4):S325\u201335.","journal-title":"Clin Infect Dis"},{"issue":"3","key":"5151_CR34","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1128\/MMBR.00078-15","volume":"80","author":"MK Paczosa","year":"2016","unstructured":"Paczosa MK, Mecsas J. Klebsiella pneumoniae: going on the offense with a strong defense. Microbiol Mol Biol Rev. 2016;80(3):629\u201361.","journal-title":"Microbiol Mol Biol Rev"},{"key":"5151_CR35","doi-asserted-by":"publisher","unstructured":"Moura Q, Esposito F, Fernandes MR, Espinoza-Munoz M, Souza TA, Santos SR, Cerdeira L, Cassettari V, Lincopan N: Genome sequence analysis of a hypermucoviscous\/hypervirulent and MDR CTX-M-15\/K19\/ST29 Klebsiella pneumoniae isolated from human infection. Pathog Dis. 2017;75(9). https:\/\/doi.org\/10.1093\/femspd\/ftx121.","DOI":"10.1093\/femspd\/ftx121"},{"issue":"1","key":"5151_CR36","doi-asserted-by":"publisher","first-page":"80","DOI":"10.20411\/pai.v10i1.777","volume":"10","author":"D Al Ismail","year":"2024","unstructured":"Al Ismail D, Campos-Madueno EI, Dona V, Endimiani A. Hypervirulent Klebsiella pneumoniae (hvKp): overview, epidemiology, and laboratory detection. Pathog Immun. 2024;10(1):80\u2013119.","journal-title":"Pathog Immun"},{"issue":"1","key":"5151_CR37","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3201\/eid3101.241516","volume":"31","author":"TA Russo","year":"2025","unstructured":"Russo TA, Lebreton F, McGann PT. A step forward in hypervirulent Klebsiella pneumoniae diagnostics. Emerg Infect Dis. 2025;31(1):1\u20133.","journal-title":"Emerg Infect Dis"},{"key":"5151_CR38","doi-asserted-by":"publisher","unstructured":"Wami H, Wallenstein A, Sauer D, Stoll M, von Bunau R, Oswald E, Muller R, Dobrindt U: Insights into evolution and coexistence of the colibactin- and yersiniabactin secondary metabolite determinants in enterobacterial populations. Microb Genom. 2021;7(6). https:\/\/doi.org\/10.1099\/mgen.0.000577.","DOI":"10.1099\/mgen.0.000577"},{"issue":"2","key":"5151_CR39","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1128\/JB.01219-07","volume":"190","author":"TL Lin","year":"2008","unstructured":"Lin TL, Lee CZ, Hsieh PF, Tsai SF, Wang JT. Characterization of integrative and conjugative element ICEKp1-associated genomic heterogeneity in a Klebsiella pneumoniae strain isolated from a primary liver abscess. J Bacteriol. 2008;190(2):515\u201326.","journal-title":"J Bacteriol"},{"issue":"27","key":"5151_CR40","doi-asserted-by":"publisher","first-page":"E3574","DOI":"10.1073\/pnas.1501049112","volume":"112","author":"KE Holt","year":"2015","unstructured":"Holt KE, Wertheim H, Zadoks RN, Baker S, Whitehouse CA, Dance D, et al. Genomic analysis of diversity, population structure, virulence, and antimicrobial resistance in Klebsiella pneumoniae, an urgent threat to public health. Proc Natl Acad Sci U S A. 2015;112(27):E3574-3581.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"8","key":"5151_CR41","doi-asserted-by":"publisher","first-page":"3325","DOI":"10.1128\/IAI.00430-15","volume":"83","author":"TA Russo","year":"2015","unstructured":"Russo TA, Olson R, MacDonald U, Beanan J, Davidson BA. Aerobactin, but not yersiniabactin, salmochelin, or enterobactin, enables the growth\/survival of hypervirulent (hypermucoviscous) Klebsiella pneumoniae ex vivo and in vivo. Infect Immun. 2015;83(8):3325\u201333.","journal-title":"Infect Immun"}],"container-title":["BMC Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12866-026-05151-z","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12866-026-05151-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12866-026-05151-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T18:07:07Z","timestamp":1785521227000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1186\/s12866-026-05151-z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,29]]},"references-count":41,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["5151"],"URL":"https:\/\/doi.org\/10.1186\/s12866-026-05151-z","relation":{},"ISSN":["1471-2180"],"issn-type":[{"value":"1471-2180","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,29]]},"assertion":[{"value":"26 December 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 May 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 May 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"This study received approval from the Ethical Committee of Shanghai Pulmonary Hospital. Informed consent was obtained from all participants involved in the study. This research complied with the Declaration of Helsinki.","order":1,"name":"Ethics","label":"Ethics approval and consent to participate","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","label":"Consent for publication","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"678"}}