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Oxygen levels during CNS infections are unknown. Here we established and evaluated an in vivo measurement system to characterize the oxygen level in parallel with bacterial numbers (CFU\/mL), the cell number and pH level inside the CSF of healthy compared to <jats:italic>Streptococcus suis<\/jats:italic>-infected pigs. The animals were anesthetized over a seven-hour period with isoflurane in air\/oxygen at physiologic arterial partial pressure of oxygen. Oxygen levels in CSF of anesthetized pigs were compared to euthanized pigs. The detected partial pressure of oxygen in the CSF remained constant in a range of 47\u201363\u00a0mmHg, independent of the infection status (bacterial or cell number). In contrast, the pH value showed a slight drop during infection, which correlated with cell and bacterial number in CSF. We present physiologic oxygen and pH values in CSF during the onset of bacterial meningitis.<\/jats:p>","DOI":"10.1186\/s12868-021-00648-x","type":"journal-article","created":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T13:03:17Z","timestamp":1624885397000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["In vivo oxygen measurement in cerebrospinal fluid of pigs to determine physiologic and pathophysiologic oxygen values during CNS infections"],"prefix":"10.1186","volume":"22","author":[{"given":"Nicole","family":"de Buhr","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Martens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marita","family":"Meurer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta C.","family":"Bonilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franz","family":"S\u00f6bbeler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara","family":"Twele","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Neudeck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Wendt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Beineke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"K\u00e4stner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maren","family":"von K\u00f6ckritz-Blickwede","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,6,28]]},"reference":[{"key":"648_CR1","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1186\/s40348-016-0041-y","volume":"3","author":"NE Zeitouni","year":"2016","unstructured":"Zeitouni NE, Chotikatum S, von K\u00f6ckritz-Blickwede M, Naim HY. 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Vet Microbiol. 2006;115:117\u201327. https:\/\/doi.org\/10.1016\/j.vetmic.2005.12.013.","journal-title":"Vet Microbiol"}],"container-title":["BMC Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12868-021-00648-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12868-021-00648-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12868-021-00648-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T13:04:36Z","timestamp":1624885476000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcneurosci.biomedcentral.com\/articles\/10.1186\/s12868-021-00648-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,28]]},"references-count":53,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["648"],"URL":"https:\/\/doi.org\/10.1186\/s12868-021-00648-x","relation":{},"ISSN":["1471-2202"],"issn-type":[{"value":"1471-2202","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,28]]},"assertion":[{"value":"14 March 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 June 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 June 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The handling and treatment of all animals were conducted in strict accordance with the principles outlined in the EU Directive 2010\/63\/EU and the German Animal Protection Law (Tierschutzgesetz). All application techniques and sampling of blood conducted in this study were in line with the recommendations of the German Society for Laboratory Animal Science (Gesellschaft f\u00fcr Versuchstierkunde) and the German Veterinary Association for the Protection of Animals (Tier\u00e4rztliche Vereinigung f\u00fcr Tierschutz e. V.) ( \/index.html).Part A: Euthanasia of pigs was approved and registered by the local Animal Welfare Officer at University of Veterinary Medicine Hannover in accordance with the German Animal Welfare Law under number TiHo-T-2019-14.Part B: This part of this study was approved by the authorities in the committee on animal experiments of the Lower Saxonian State Office for Consumer Protection and Food Safety (Nieders\u00e4chsisches Landesamt f\u00fcr Verbraucherschutz und Lebensmittelsicherheit (LAVES), Lower Saxonian, Germany under the registration number 33.8-42502-04-18\/2879.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent for publication"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"45"}}