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Due to the paucity of information on the appropriate monofilament size for inducing MCAO in SD rats and the importance of including middle-aged models in ischemic stroke studies, we aimed to: (i). determine an appropriate Doccol<jats:sup>\u00ae<\/jats:sup> monofilament size for middle-aged male SD rats which weighed\u2009&gt;\u2009500\u00a0g following 24-h transient MCAO survival as well as (ii). demonstrate the optimal Doccol<jats:sup>\u00ae<\/jats:sup> filament size for middle-aged males (\u2264\u2009500\u00a0g) and females (273\u2013300\u00a0g) while using young adult male SD rats (372\u2013472\u00a0g) as control for severity of infarct volume following 7-days post-MCAO. All rats were subjected to 90-min transient MCAO. We show that 0.43\u00a0mm Doccol<jats:sup>\u00ae<\/jats:sup> monofilament size is more appropriate to induce large infarct lesion and optimal functional deficit when compared to 0.45\u00a0mm and 0.47\u00a0mm at 24\u00a0h post-MCAO. Our data on infarct volumes at 7\u00a0days post-MCAO as well as the observed weight loss and functional deficits at post-MCAO days 1, 3 and 7 demonstrate that 0.41\u00a0mm, 0.37\u00a0mm and 0.39\u00a0mm are optimal Doccol<jats:sup>\u00ae<\/jats:sup> filament sizes for middle-aged male (477.3\u2009\u00b1\u200939.61\u00a0g) and female (302.6\u2009\u00b1\u200926.28\u00a0g) as well as young-adult male (362.2\u2009\u00b1\u200928.38\u00a0g) SD rats, respectively.<\/jats:p>","DOI":"10.1186\/s12868-022-00764-2","type":"journal-article","created":{"date-parts":[[2022,12,9]],"date-time":"2022-12-09T09:03:14Z","timestamp":1670576594000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Optimizing intraluminal monofilament model of ischemic stroke in middle-aged Sprague\u2013Dawley rats"],"prefix":"10.1186","volume":"23","author":[{"given":"I. 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