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We investigated how H<jats:sub>2<\/jats:sub>S ameliorates iron (Fe) deficiency in soybean (<jats:italic>Glycine max<\/jats:italic> L.) seedlings. Multidisciplinary approaches including physiological, biochemical and molecular, and transcriptome methods were used to investigate the H<jats:sub>2<\/jats:sub>S role in regulating Fe availability in soybean seedlings.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>Our results showed that H<jats:sub>2<\/jats:sub>S completely prevented leaf interveinal chlorosis and caused an increase in soybean seedling biomass under Fe deficiency conditions. Moreover, H<jats:sub>2<\/jats:sub>S decreased the amount of root-bound apoplastic Fe and increased the Fe content in leaves and roots by regulating the ferric-chelate reductase (FCR) activities and Fe homeostasis- and sulphur metabolism-related gene expression levels, thereby promoting photosynthesis in soybean seedlings. In addition, H<jats:sub>2<\/jats:sub>S changed the plant hormone concentrations by modulating plant hormone-related gene expression abundances in soybean seedlings grown in Fe-deficient solution. Furthermore, organic acid biosynthesis and related genes expression also played a vital role in modulating the H<jats:sub>2<\/jats:sub>S-mediated alleviation of Fe deficiency in soybean seedlings.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusion<\/jats:title>\n<jats:p>Our results indicated that Fe deficiency was alleviated by H<jats:sub>2<\/jats:sub>S through enhancement of Fe acquisition and assimilation, thereby regulating plant hormones and organic acid synthesis in plants.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12870-020-02601-2","type":"journal-article","created":{"date-parts":[[2020,8,20]],"date-time":"2020-08-20T13:04:32Z","timestamp":1597928672000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Hydrogen sulphide alleviates iron deficiency by promoting iron availability and plant hormone levels in Glycine max seedlings"],"prefix":"10.1186","volume":"20","author":[{"given":"Juan","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ni-Na","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue-Yuan","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhouping","family":"Shangguan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Hua","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge-Hong","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,8,20]]},"reference":[{"key":"2601_CR1","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1038\/88067","volume":"19","author":"ML Guerinot","year":"2001","unstructured":"Guerinot ML. 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