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Despite the diversity of FLAs, only two members, Arabidopsis\n                      <jats:italic>FLA3<\/jats:italic>\n                      and rice\n                      <jats:italic>MTR1<\/jats:italic>\n                      , are reported to be involved in sexual reproduction. In this study, another Arabidopsis FLA-encoding gene,\n                      <jats:italic>FLA14<\/jats:italic>\n                      , was identified, and its role was investigated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Arabidopsis\n                      <jats:italic>FLA14<\/jats:italic>\n                      was found to be a pollen grain-specific gene. Expression results from fusion with green fluorescent protein showed that FLA14 was localized along the cell membrane and in Hechtian strands. A loss-of-function mutant of\n                      <jats:italic>FLA14<\/jats:italic>\n                      showed no discernible defects during male gametogenesis, but precocious pollen germination occurred inside the mature anthers under high moisture conditions. Overexpression of\n                      <jats:italic>FLA14<\/jats:italic>\n                      caused 39.2% abnormal pollen grains with a shrunken and withered appearance, leading to largely reduced fertility with short mature siliques and lower seed set. Cytological and ultramicroscopic observation showed that ectopic expression of\n                      <jats:italic>FLA14<\/jats:italic>\n                      caused disruption at the uninucleate stage, resulting in either collapsed pollen with absent intine or pollen of normal appearance but with a thickened intine.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Taken together, our data suggest a role for\n                      <jats:italic>FLA14<\/jats:italic>\n                      in pollen development and preventing premature pollen germination inside the anthers under high relative humidity in Arabidopsis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12870-021-03038-x","type":"journal-article","created":{"date-parts":[[2021,6,3]],"date-time":"2021-06-03T07:09:23Z","timestamp":1622704163000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["FLA14 is required for pollen development and preventing premature pollen germination under high humidity in Arabidopsis"],"prefix":"10.1186","volume":"21","author":[{"given":"Yingjing","family":"Miao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiashu","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youjian","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sue","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,6,3]]},"reference":[{"key":"3038_CR1","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1007\/978-3-642-02301-9_12","volume-title":"Plant Developmental Biology-Biotechnological Perspectives","author":"D Twell","year":"2010","unstructured":"Twell D. 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