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Leaf development directly determines the growth, flowering and fruiting of plants. There are many factors that affect leaf development, such as the growth environment, gene expression, and hormone synthesis. In this study, tomatoes were used to study the role of the transcription factor\n                      <jats:italic>Solanum lycopersicum<\/jats:italic>\n                      salt-related MYB1-like (SlSRM1-like) in the development of tomato leaves.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Loss-of-function of the\n                      <jats:italic>SlSRM1-like<\/jats:italic>\n                      gene mediated by clustered, regularly interspaced, short palindromic repeat (CRISPR)\/CRISPR-associated 9 (Cas9) resulted in abnormal tomato leaf morphology, including thinner leaves, wrinkled edges, raised veins, disordered edge veins, and left and right asymmetry. An analysis of the transcription levels of genes related to leaf development revealed that the expression of these genes was significantly altered in the\n                      <jats:italic>SlSRM1-like<\/jats:italic>\n                      mutants (\n                      <jats:italic>SlSRM1-like<\/jats:italic>\n                      -Ms). Moreover, the\n                      <jats:italic>SlSRM1-like<\/jats:italic>\n                      gene was expressed at higher transcription levels in young tissues than in old tissues, and its expression was also induced in response to auxin. In addition, the transcription levels of genes related to the auxin pathway, which regulates tomato growth and development, were severely affected in the\n                      <jats:italic>SlSRM1-like<\/jats:italic>\n                      -Ms. Therefore, it is hypothesized that the\n                      <jats:italic>SlSRM1-like<\/jats:italic>\n                      gene functions in the regulation of tomato leaf development through the auxin-related pathway.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      In this study, we successfully knocked out the\n                      <jats:italic>SlSRM1-like<\/jats:italic>\n                      gene in the tomato variety Ailsa Craig using CRISPR technology and found that knockout of the\n                      <jats:italic>SlSRM1-like<\/jats:italic>\n                      gene resulted in abnormal development of tomato leaves. Further research indicated that\n                      <jats:italic>SlSRM1-like<\/jats:italic>\n                      regulated tomato leaf development through auxin-related pathways. The results provide an important reference for the functional study of other\n                      <jats:italic>SRM1-like<\/jats:italic>\n                      genes in plants and provide new insights into the regulation of leaf development in tomato and other plants.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12870-021-03397-5","type":"journal-article","created":{"date-parts":[[2022,1,3]],"date-time":"2022-01-03T03:06:58Z","timestamp":1641179218000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["CRISPR-Cas9-mediated mutagenesis of the SlSRM1-like gene leads to abnormal leaf development in tomatoes"],"prefix":"10.1186","volume":"22","author":[{"given":"Yao","family":"Tang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijia","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqing","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hexuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyang","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingfu","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanhuan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingbin","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,1,3]]},"reference":[{"key":"3397_CR1","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1016\/j.pbi.2011.02.001","volume":"14","author":"Z Peleg","year":"2011","unstructured":"Peleg Z, Blumwald E. 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