{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T10:22:15Z","timestamp":1792059735408,"version":"4.4.0"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,9,16]],"date-time":"2021-09-16T00:00:00Z","timestamp":1631750400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,9,16]],"date-time":"2021-09-16T00:00:00Z","timestamp":1631750400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81701300"],"award-info":[{"award-number":["81701300"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31771070"],"award-info":[{"award-number":["31771070"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Cardiovasc Disord"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Purpose<\/jats:title>\n                <jats:p>Previous clinical studies have suggested an effect of gender on outcome after out-of-hospital cardiac arrest, but the results are conflicting and there is no uniform agreement regarding gender differences in survival and prognosis. The present study was aimed to investigate the interaction between gender and post resuscitation interventions on neurological outcome in an asphyxial rat model of cardiac arrest.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Asphyxia was induced by blocking the endotracheal tube in 120 adult Sprague\u2013Dawley rats (60 males and 60 females) at the same age. Cardiopulmonary resuscitation (CPR) was started after 5\u00a0min of untreated cardiac arrest. Animals were randomized into one of the three post resuscitation care intervention groups (n\u2009=\u200940, 20 males) immediately after resuscitation: (1) normothermic control (NC): ventilated with 2% N<jats:sub>2<\/jats:sub>\/98% O<jats:sub>2<\/jats:sub> for 1\u00a0h under normothermia; (2) targeted temperature management (TTM): ventilated with 2% N<jats:sub>2<\/jats:sub>\/98% O<jats:sub>2<\/jats:sub> for 1\u00a0h under hypothermia; (3) hydrogen inhalation (HI): ventilated with 2% H<jats:sub>2<\/jats:sub>\/98% O<jats:sub>2<\/jats:sub> for 1\u00a0h under normothermia. Physiological variables were recorded during the 5\u00a0h post resuscitation monitoring period. Neurological deficit score (NDS) and accumulative survival were used to assess 96\u00a0h outcomes. Mutual independence analysis and Mantel\u2013Haenszel stratified analysis were used to explore the associations among gender, intervention and survival.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The body weights of female rats were significantly lighter than males, but CPR characteristics did not differ between genders. Compared with male rats, females had significantly lower mean arterial pressure, longer onset time of the electroencephalogram (EEG) burst and time to normal EEG trace (TTNT) in the NC group; relatively longer TTNT in the TTM group; and substantially longer TTNT, lower NDSs, and higher survival in the HI group. Mutual independence analysis revealed that both gender and intervention were associated with neurological outcome. Mantel\u2013Haenszel stratified analysis demonstrated that female rats had significantly higher survival rate than males when adjusted for the confounder intervention.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>In this rat model cardiac arrest and CPR, gender did not affect resuscitation but associated with neurological outcome. The superiority of female rats in neurological recovery was affected by post resuscitation interventions and female rats were more likely to benefit from hydrogen therapy.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12872-021-02262-5","type":"journal-article","created":{"date-parts":[[2021,9,16]],"date-time":"2021-09-16T10:03:27Z","timestamp":1631786607000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Interaction between gender and post resuscitation interventions on neurological outcome in an asphyxial rat model of cardiac arrest"],"prefix":"10.1186","volume":"21","author":[{"given":"Jianjie","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingru","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bihua","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiming","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaifa","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changlin","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongqin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,9,16]]},"reference":[{"key":"2262_CR1","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.resuscitation.2020.02.044","volume":"152","author":"T Kiguchi","year":"2020","unstructured":"Kiguchi T, Okubo M, Nishiyama C, Maconochie I, Ong MEH, Kern KB, et al. Out-of-hospital cardiac arrest across the world: first report from the International Liaison Committee on Resuscitation (ILCOR). Resuscitation. 2020;152:39\u201349.","journal-title":"Resuscitation"},{"key":"2262_CR2","doi-asserted-by":"publisher","first-page":"636651","DOI":"10.3389\/fmed.2021.636651","volume":"8","author":"RC Choudhary","year":"2021","unstructured":"Choudhary RC, Shoaib M, Sohnen S, Rolston DM, Jafari D, Miyara SJ, et al. Pharmacological approach for neuroprotection after cardiac arrest-a narrative review of current therapies and future neuroprotective cocktail. Front Med Lausanne. 2021;8:636651.","journal-title":"Front Med Lausanne"},{"issue":"1","key":"2262_CR3","first-page":"36","volume":"7","author":"E Navab","year":"2019","unstructured":"Navab E, Esmaeili M, Poorkhorshidi N, Salimi R, Khazaei A, Moghimbeigi A. Predictors of out of hospital cardiac arrest outcomes in pre-hospital settings; a retrospective cross-sectional study. Arch Acad Emerg Med. 2019;7(1):36.","journal-title":"Arch Acad Emerg Med"},{"key":"2262_CR4","doi-asserted-by":"publisher","first-page":"S366","DOI":"10.1161\/CIR.0000000000000916","volume":"142","author":"AR Panchal","year":"2020","unstructured":"Panchal AR, Bartos JA, Caba\u00f1as JG, Donnino MW, Drennan IR, Hirsch KG, et al. Adult basic and advanced life support writing group Part 3: adult basic and advanced life support: 2020 American Heart Association Guidelines for cardiopulmonary resuscitation and emergency cardiovascular care. Circulation. 2020;142:S366\u2013468.","journal-title":"Circulation"},{"issue":"2","key":"2262_CR5","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1007\/s00134-018-5487-z","volume":"45","author":"Y Helviz","year":"2019","unstructured":"Helviz Y, Ong M, Einav S. Cardiac arrest, gender and resuscitation outcomes. Intensive Care Med. 2019;45(2):278\u201381.","journal-title":"Intensive Care Med"},{"issue":"1","key":"2262_CR6","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1186\/s13054-020-03331-5","volume":"24","author":"H Lei","year":"2020","unstructured":"Lei H, Hu J, Liu L, Xu D. Sex differences in survival after out-of-hospital cardiac arrest: a meta-analysis. Crit Care. 2020;24(1):613.","journal-title":"Crit Care"},{"issue":"3","key":"2262_CR7","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/S0300-9572(99)00022-2","volume":"40","author":"E Perers","year":"1999","unstructured":"Perers E, Abrahamsson P, B\u00e5ng A, Engdahl J, Lindqvist J, Karlson BW, et al. There is a difference in characteristics and outcome between women and men who suffer out of hospital cardiac arrest. Resuscitation. 1999;40(3):133\u201340.","journal-title":"Resuscitation"},{"issue":"2","key":"2262_CR8","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1016\/j.resuscitation.2003.09.012","volume":"60","author":"J Herlitz","year":"2004","unstructured":"Herlitz J, Engdahl J, Svensson L, Young M, Angquist KA, Holmberg S. Is female sex associated with increased survival after out-of-hospital cardiac arrest? Resuscitation. 2004;60(2):197\u2013203.","journal-title":"Resuscitation"},{"issue":"9","key":"2262_CR9","doi-asserted-by":"publisher","first-page":"004131","DOI":"10.1161\/JAHA.116.004131","volume":"5","author":"N Bosson","year":"2016","unstructured":"Bosson N, Kaji AH, Fang A, Thomas JL, French WJ, Shavelle D, et al. Sex differences in survival from out-of-hospital cardiac arrest in the era of regionalized systems and advanced post-resuscitation care. J Am Heart Assoc. 2016;5(9):004131.","journal-title":"J Am Heart Assoc."},{"issue":"47","key":"2262_CR10","doi-asserted-by":"publisher","first-page":"3824","DOI":"10.1093\/eurheartj\/ehz297","volume":"40","author":"MT Blom","year":"2019","unstructured":"Blom MT, Oving I, Berdowski J, van Valkengoed IGM, Bardai A, Tan HL. Women have lower chances than men to be resuscitated and survive out-of-hospital cardiac arrest. Eur Heart J. 2019;40(47):3824\u201334.","journal-title":"Eur Heart J"},{"issue":"3","key":"2262_CR11","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1097\/SHK.0000000000000585","volume":"46","author":"P Wang","year":"2016","unstructured":"Wang P, Jia L, Chen B, Zhang L, Liu J, Long J, et al. Hydrogen inhalation is superior to mild hypothermia in improving cardiac function and neurological outcome in an asphyxial cardiac arrest model of rats. Shock. 2016;46(3):312\u20138.","journal-title":"Shock"},{"issue":"1","key":"2262_CR12","doi-asserted-by":"publisher","first-page":"16500","DOI":"10.1038\/s41598-019-52477-y","volume":"9","author":"T Hu","year":"2019","unstructured":"Hu T, Wang J, Wang S, Li J, Chen B, Zuo F, et al. Effects of the duration of postresuscitation hyperoxic ventilation on neurological outcome and survival in an asphyxial cardiac arrest rat model. Sci Rep. 2019;9(1):16500.","journal-title":"Sci Rep"},{"issue":"2","key":"2262_CR13","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/0300-9572(84)90063-7","volume":"12","author":"HH Hendrickx","year":"1984","unstructured":"Hendrickx HH, Safar P, Miller A. Asphyxia, cardiac arrest and resuscitation in rats. II Long term behavioral changes. Resuscitation. 1984;12(2):117\u201328.","journal-title":"Resuscitation"},{"key":"2262_CR14","doi-asserted-by":"publisher","DOI":"10.1201\/b15072","volume-title":"The analysis of contingency tables","author":"BS Everitt","year":"1992","unstructured":"Everitt BS. The analysis of contingency tables. 2nd ed. London: Chapman and Hall Ltd; 1992.","edition":"2"},{"issue":"22","key":"2262_CR15","doi-asserted-by":"publisher","first-page":"2699","DOI":"10.1161\/hc4701.099784","volume":"104","author":"C Kim","year":"2001","unstructured":"Kim C, Fahrenbruch CE, Cobb LA, Eisenberg MS. Out-of-hospital cardiac arrest in men and women. Circulation. 2001;104(22):2699\u2013703.","journal-title":"Circulation"},{"key":"2262_CR16","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1016\/j.resuscitation.2016.03.002","volume":"102","author":"YY Ng","year":"2016","unstructured":"Ng YY, Wah W, Liu N, Zhou SA, Ho AF, Pek PP, et al. PAROS Clinical Research Network. Associations between gender and cardiac arrest outcomes in Pan-Asian out-of-hospital cardiac arrest patients. Resuscitation. 2016;102:116\u201321.","journal-title":"Resuscitation"},{"issue":"2","key":"2262_CR17","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1016\/j.whi.2018.10.007","volume":"29","author":"JF Lewis","year":"2019","unstructured":"Lewis JF, Zeger SL, Li X, Mann NC, Newgard CD, Haynes S, et al. Gender differences in the quality of EMS care nationwide for chest pain and out-of-hospital cardiac arrest. Womens Health Issues. 2019;29(2):116\u201324.","journal-title":"Womens Health Issues"},{"issue":"12","key":"2262_CR18","doi-asserted-by":"publisher","first-page":"1503","DOI":"10.1111\/acem.12540","volume":"21","author":"B Safdar","year":"2014","unstructured":"Safdar B, Stolz U, Stiell IG, Cone DC, Bobrow BJ, deBoehr M, et al. Differential survival for men and women from out-of-hospital cardiac arrest varies by age: results from the OPALS study. Acad Emerg Med. 2014;21(12):1503\u201311.","journal-title":"Acad Emerg Med"},{"issue":"4","key":"2262_CR19","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1016\/j.amjmed.2010.10.020","volume":"124","author":"M Akahane","year":"2011","unstructured":"Akahane M, Ogawa T, Koike S, Tanabe S, Horiguchi H, Mizoguchi T, et al. The effects of sex on out-of-hospital cardiac arrest outcomes. Am J Med. 2011;124(4):325\u201333.","journal-title":"Am J Med"},{"issue":"2","key":"2262_CR20","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1016\/j.resuscitation.2004.10.017","volume":"65","author":"S Mahapatra","year":"2005","unstructured":"Mahapatra S, Bunch TJ, White RD, Hodge DO, Packer DL. Sex differences in outcome after ventricular fibrillation in out-of-hospital cardiac arrest. Resuscitation. 2005;65(2):197\u2013202.","journal-title":"Resuscitation"},{"issue":"1","key":"2262_CR21","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1186\/s13054-015-0904-y","volume":"19","author":"V Karlsson","year":"2015","unstructured":"Karlsson V, Dankiewicz J, Nielsen N, Kern KB, Mooney MR, Riker RR, et al. Association of gender to outcome after out-of-hospital cardiac arrest\u2013a report from the International Cardiac Arrest Registry. Crit Care. 2015;19(1):182.","journal-title":"Crit Care"},{"issue":"6","key":"2262_CR22","doi-asserted-by":"publisher","first-page":"e003704","DOI":"10.1161\/JAHA.116.003704","volume":"5","author":"LK Kim","year":"2016","unstructured":"Kim LK, Looser P, Swaminathan RV, Horowitz J, Friedman O, Shin JH, et al. Sex-based disparities in incidence, treatment, and outcomes of cardiac arrest in the United States, 2003\u20132012. J Am Heart Assoc. 2016;5(6):e003704.","journal-title":"J Am Heart Assoc"},{"issue":"5","key":"2262_CR23","doi-asserted-by":"publisher","first-page":"414","DOI":"10.1177\/2048872617696368","volume":"7","author":"M Winther-Jensen","year":"2018","unstructured":"Winther-Jensen M, Hassager C, Kjaergaard J, Bro-Jeppesen J, Thomsen JH, Lippert FK, et al. Women have a worse prognosis and undergo fewer coronary angiographies after out-of-hospital cardiac arrest than men. Eur Heart J Acute Cardiovasc Care. 2018;7(5):414\u201322.","journal-title":"Eur Heart J Acute Cardiovasc Care"},{"issue":"6","key":"2262_CR24","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1016\/j.ajem.2014.02.004","volume":"32","author":"MR Greenberg","year":"2014","unstructured":"Greenberg MR, Ahnert AM, Patel NC, Bennett CE, Elliott N, Lundquist M, et al. Sex differences in cardiac arrest survivors who receive therapeutic hypothermia. Am J Emerg Med. 2014;32(6):545\u20138.","journal-title":"Am J Emerg Med"},{"issue":"1","key":"2262_CR25","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1186\/s13054-017-1860-5","volume":"21","author":"SH Oh","year":"2017","unstructured":"Oh SH, Park KN, Lim J, Choi SP, Oh JS, Cho IS, et al. Korean hypothermia network investigators. The impact of sex and age on neurological outcomes (hospital discharge) in out-of-hospital cardiac arrest patients with targeted temperature management. Crit Care. 2017;21(1):272.","journal-title":"Crit Care"},{"issue":"1","key":"2262_CR26","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1080\/02699052.2019.1680866","volume":"34","author":"A Fillbrandt","year":"2020","unstructured":"Fillbrandt A, Frank B. Gender differences in cognitive outcome after cardiac arrest: a retrospective cohort study. Brain Inj. 2020;34(1):122\u201330.","journal-title":"Brain Inj"},{"key":"2262_CR27","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1016\/j.resuscitation.2015.06.030","volume":"96","author":"M Winther-Jensen","year":"2015","unstructured":"Winther-Jensen M, Kjaergaard J, Wanscher M, Nielsen N, Wetterslev J, Cronberg T, et al. No difference in mortality between men and women after out-of-hospital cardiac arrest. Resuscitation. 2015;96:78\u201384.","journal-title":"Resuscitation"},{"issue":"6","key":"2262_CR28","doi-asserted-by":"publisher","first-page":"689","DOI":"10.1097\/SHK.0000000000001092","volume":"50","author":"G Chen","year":"2018","unstructured":"Chen G, Chen B, Dai C, Wang J, Wang J, Huang Y, et al. Hydrogen inhalation is superior to mild hypothermia for improving neurological outcome and survival in a cardiac arrest model of spontaneously hypertensive rat. Shock. 2018;50(6):689\u201395.","journal-title":"Shock"},{"issue":"1","key":"2262_CR29","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1097\/CCM.0000000000003474","volume":"47","author":"BW Roberts","year":"2019","unstructured":"Roberts BW, Kilgannon JH, Hunter BR, Puskarich MA, Shea L, Donnino M, et al. Association between elevated mean arterial blood pressure and neurologic outcome after resuscitation from cardiac arrest: results from a multicenter prospective cohort study. Crit Care Med. 2019;47(1):93\u2013100.","journal-title":"Crit Care Med"},{"issue":"12","key":"2262_CR30","doi-asserted-by":"publisher","first-page":"1094","DOI":"10.1161\/CIRCULATIONAHA.115.015754","volume":"132","author":"SH Oh","year":"2015","unstructured":"Oh SH, Park KN, Shon YM, Kim YM, Kim HJ, Youn CS, et al. Continuous amplitude-integrated electroencephalographic monitoring is a useful prognostic tool for hypothermia-treated cardiac arrest patients. Circulation. 2015;132(12):1094\u2013103.","journal-title":"Circulation"},{"issue":"4","key":"2262_CR31","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1097\/00004647-200004000-00001","volume":"20","author":"PD Hurn","year":"2000","unstructured":"Hurn PD, Macrae IM. Estrogen as a neuroprotectant in stroke. J Cereb Blood Flow Metab. 2000;20(4):631\u201352.","journal-title":"J Cereb Blood Flow Metab"},{"key":"2262_CR32","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1016\/j.neuroscience.2016.03.052","volume":"325","author":"RM Dietz","year":"2016","unstructured":"Dietz RM, Deng G, Orfila JE, Hui X, Traystman RJ, Herson PS. Therapeutic hypothermia protects against ischemia-induced impairment of synaptic plasticity following juvenile cardiac arrest in sex-dependent manner. Neuroscience. 2016;325:132\u201341.","journal-title":"Neuroscience"},{"issue":"29","key":"2262_CR33","doi-asserted-by":"publisher","first-page":"5430","DOI":"10.2174\/0929867326666190506124836","volume":"26","author":"K Kurisu","year":"2019","unstructured":"Kurisu K, Kim JY, You J, Yenari MA. Therapeutic hypothermia and neuroprotection in acute neurological disease. Curr Med Chem. 2019;26(29):5430\u201355.","journal-title":"Curr Med Chem"},{"key":"2262_CR34","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1016\/bs.mie.2014.11.038","volume":"555","author":"S Ohta","year":"2015","unstructured":"Ohta S. Molecular hydrogen as a novel antioxidant: overview of the advantages of hydrogen for medical applications. Methods Enzymol. 2015;555:289\u2013317.","journal-title":"Methods Enzymol"},{"issue":"11\u201312","key":"2262_CR35","doi-asserted-by":"publisher","first-page":"1311","DOI":"10.1080\/10715762.2018.1460749","volume":"52","author":"C Hou","year":"2018","unstructured":"Hou C, Peng Y, Qin C, Fan F, Liu J, Long J. Hydrogen-rich water improves cognitive impairment gender-dependently in APP\/PS1 mice without affecting A\u03b2 clearance. Free Radic Res. 2018;52(11\u201312):1311\u201322.","journal-title":"Free Radic Res"},{"issue":"2","key":"2262_CR36","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1016\/S0300-9572(03)00025-X","volume":"57","author":"KH Reid","year":"2003","unstructured":"Reid KH, Paskitti M, Guo SZ, Schmelzer T, Iyer V. Experience with ketamine and sodium pentobarbital as anesthetics in a rat model of cardiac arrest and resuscitation. Resuscitation. 2003;57(2):201\u201310.","journal-title":"Resuscitation"},{"key":"2262_CR37","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1016\/j.ajem.2020.08.103","volume":"39","author":"K Bornstein","year":"2021","unstructured":"Bornstein K, Long B, Porta AD, Weinberg G. After a century, Epinephrine\u2019s role in cardiac arrest resuscitation remains controversial. Am J Emerg Med. 2021;39:168\u201372.","journal-title":"Am J Emerg Med"}],"container-title":["BMC Cardiovascular Disorders"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12872-021-02262-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12872-021-02262-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12872-021-02262-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,16]],"date-time":"2021-09-16T10:05:15Z","timestamp":1631786715000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmccardiovascdisord.biomedcentral.com\/articles\/10.1186\/s12872-021-02262-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,16]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["2262"],"URL":"https:\/\/doi.org\/10.1186\/s12872-021-02262-5","relation":{},"ISSN":["1471-2261"],"issn-type":[{"value":"1471-2261","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,16]]},"assertion":[{"value":"31 May 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 September 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 September 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This study was approved by the Laboratory Animal Welfare and Ethics Committee of the Army Medical University (Approve No: AMUWEC2019482) and was conducted in accordance with the ARRIVE guidelines. In addition to this, all methods were performed in accordance with the relevant guidelines and regulations.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"441"}}