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ABCA1 and ABCG1 transporters regulate cell cholesterol content and reverse cholesterol transport. We aimed to determine whether DNA methylation and mRNA levels of the <jats:italic>ABCA1<\/jats:italic> and <jats:italic>ABCG1<\/jats:italic> genes in EAT and subcutaneous adipose tissue (SAT) were associated with CAD.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Paired EAT and SAT samples were collected from 82 patients undergoing elective cardiac surgery either for coronary artery bypass grafting (CAD group, N\u2009=\u200966) or valve surgery (NCAD group, N\u2009=\u200916). <jats:italic>ABCA1<\/jats:italic> and <jats:italic>ABCG1<\/jats:italic> mRNA levels in EAT and SAT samples were analyzed using real time polymerase chain reaction, ABCA1 protein levels in EAT samples were assessed by western blotting. <jats:italic>ABCA1<\/jats:italic> and <jats:italic>ABCG1<\/jats:italic> DNA methylation analysis was performed in 24 samples from the CAD group and 9 samples from the NCAD group via pyrosequencing.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>DNA methylation levels in the <jats:italic>ABCA1<\/jats:italic> promoter and <jats:italic>ABCG1<\/jats:italic> cg27243685 and cg06500161 CpG sites were higher in EAT samples from patients with CAD compared with NCAD (21.92% vs 10.81%, p\u2009=\u20090.003; 71.51% vs 68.42%, p\u2009=\u20090.024; 46.11% vs 37.79%, p\u2009=\u20090.016, respectively). In patients with CAD, <jats:italic>ABCA1<\/jats:italic> and <jats:italic>ABCG1<\/jats:italic> DNA methylation levels were higher in EAT than in SAT samples (p\u2009&lt;\u20090.05). <jats:italic>ABCA1<\/jats:italic> mRNA levels in EAT samples were reduced in the subgroup of patients with CAD and concomitant carotid artery disease or peripheral artery disease compared with the NCAD group (p\u2009=\u20090.024). ABCA1 protein levels in EAT samples tended to be lower in CAD patients than in the NCAD group (p\u2009=\u20090.053). DNA methylation levels at the <jats:italic>ABCG1<\/jats:italic> cg27243685 site positively correlated with plasma triglyceride concentration (r\u2009=\u20090.510, p\u2009=\u20090.008), body mass index (r\u2009=\u20090.556, p\u2009=\u20090.013) and waist-to-hip ratio (r\u2009=\u20090.504, p\u2009=\u20090.012) in SAT samples.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>CAD is associated with <jats:italic>ABCA1<\/jats:italic> and <jats:italic>ABCG1<\/jats:italic> DNA hypermethylation in EAT. CAD with concomitant carotid artery disease or peripheral artery disease is accompanied by decreased <jats:italic>ABCA1<\/jats:italic> gene expression in EAT. DNA methylation levels at the <jats:italic>ABCG1<\/jats:italic> cg27243685 locus in SAT are associated with hypertriglyceridemia and obesity.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12872-021-02379-7","type":"journal-article","created":{"date-parts":[[2021,11,27]],"date-time":"2021-11-27T09:02:41Z","timestamp":1638003761000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["ABCA1 and ABCG1 DNA methylation in epicardial adipose tissue of patients with coronary artery disease"],"prefix":"10.1186","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3160-2314","authenticated-orcid":false,"given":"Valentina V.","family":"Miroshnikova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra A.","family":"Panteleeva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina A.","family":"Pobozheva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia D.","family":"Razgildina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina A.","family":"Polyakova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton V.","family":"Markov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga D.","family":"Belyaeva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga A.","family":"Berkovich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena I.","family":"Baranova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria S.","family":"Nazarenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valery P.","family":"Puzyrev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofya N.","family":"Pchelina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,11,27]]},"reference":[{"issue":"1","key":"2379_CR1","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1152\/physrev.00033.2011","volume":"93","author":"A Tchernof","year":"2013","unstructured":"Tchernof A, Despres JP. 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