{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T08:15:29Z","timestamp":1780992929426,"version":"3.54.1"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T00:00:00Z","timestamp":1647302400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T00:00:00Z","timestamp":1647302400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Cardiovasc Disord"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>SERPINB1 is involved in the development of a variety of diseases. The purpose of this study was to explore the effect of SERPINB1 on acute myocardial infarction (AMI).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Serum SERPINB1 level of AMI patients was measured for receiver operating characteristic curve analysis. The AMI rat model was constructed to observe myocardial damage, and the H9C2 cell oxygen glucose deprivation (OGD) model was constructed to detect cell viability. Transthoracic echocardiography was used to assess the cardiac function. TTC staining and HE staining were used to detect pathologic changes of myocardial tissues. The apoptosis of myocardial tissues and cells were measured by TUNLE staining and flow cytometry assay. CCK-8 assay to measure cell viability. SERPINB1 expression was measured by qRT-PCR. Protein expression was measured by western blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The serum SERPINB1 level was down-regulated in AMI patients. AMI modeling reduced the SERPINB1 expression level, induced inflammatory cells infiltrated, and myocardial apoptosis. OGD treatment inhibited cell viability and promoted apoptosis. The AMPK\/mTOR pathway was inhibited in AMI rats and OGD-treated H9C2 cells. Overexpression of SERPINB1 reduced infarct size and myocardial apoptosis of AMI rats, inhibited apoptosis of H9C2 cells, and activated AMPK\/mTOR pathway. However, AMPK inhibitor Dorsomorphin reversed the protective effect of SERPINB1 on myocardial cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>SERPINB1 overexpression relieved myocardial damage induced by AMI via AMPK\/mTOR pathway.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12872-022-02454-7","type":"journal-article","created":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T16:10:52Z","timestamp":1647360652000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["SERPINB1 overexpression protects myocardial damage induced by acute myocardial infarction through AMPK\/mTOR pathway"],"prefix":"10.1186","volume":"22","author":[{"given":"Hongliang","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Hua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyuan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9983-7924","authenticated-orcid":false,"given":"Ying","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,3,15]]},"reference":[{"issue":"1","key":"2454_CR1","doi-asserted-by":"publisher","first-page":"74","DOI":"10.7150\/ijms.9.74","volume":"9","author":"C Erdal","year":"2012","unstructured":"Erdal C, Karak\u00fclah G, Fermanc\u0131 E, Kunter I, Silistreli E, Canda T, et al. Early biventricular molecular responses to an acute myocardial infarction. Int J Med Sci. 2012;9(1):74\u201382.","journal-title":"Int J Med Sci"},{"key":"2454_CR2","doi-asserted-by":"publisher","first-page":"1552","DOI":"10.1161\/ATVBAHA.111.224915","volume":"32","author":"K Konstantinidis","year":"2012","unstructured":"Konstantinidis K, Whelan R, Kitsis R. Mechanisms of cell death in heart disease. Arterioscler Thromb Vasc Biol. 2012;32:1552\u201362.","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"2454_CR3","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1161\/CIRCRESAHA.111.243170","volume":"110","author":"D Marchant","year":"2012","unstructured":"Marchant D, Boyd J, Lin D, Granville D, Garmaroudi F, McManus B. Inflammation in myocardial diseases. Circ Res. 2012;110:126\u201344.","journal-title":"Circ Res"},{"key":"2454_CR4","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1016\/S0140-6736(16)30677-8","volume":"389","author":"G Reed","year":"2016","unstructured":"Reed G, Rossi J, Cannon C. Acute myocardial infarction. Lancet. 2016;389:197\u2013210.","journal-title":"Lancet"},{"issue":"36","key":"2454_CR5","doi-asserted-by":"publisher","first-page":"33293","DOI":"10.1074\/jbc.R100016200","volume":"276","author":"GA Silverman","year":"2001","unstructured":"Silverman GA, Bird PI, Carrell RW, Church FC, Coughlin PB, Gettins PGW, et al. The serpins are an expanding superfamily of structurally similar but functionally diverse proteins. J Biol Chem. 2001;276(36):33293\u20136.","journal-title":"J Biol Chem"},{"key":"2454_CR6","doi-asserted-by":"publisher","first-page":"1042","DOI":"10.2741\/1862","volume":"11","author":"J Richardson","year":"2006","unstructured":"Richardson J, Viswanathan K, Lucas A. Serpins, the vasculature, and viral therapeutics. Front Biosci. 2006;11:1042\u201356.","journal-title":"Front Biosci"},{"issue":"12","key":"2454_CR7","doi-asserted-by":"publisher","first-page":"5635","DOI":"10.1073\/pnas.89.12.5635","volume":"89","author":"E Remold-O'Donnell","year":"1992","unstructured":"Remold-O\u2019Donnell E, Chin J, Alberts M. Sequence and molecular characterization of human monocyte\/neutrophil elastase inhibitor. Proc Natl Acad Sci USA. 1992;89(12):5635\u20139.","journal-title":"Proc Natl Acad Sci USA"},{"issue":"1","key":"2454_CR8","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1016\/j.cmet.2015.12.001","volume":"23","author":"A El Ouaamari","year":"2016","unstructured":"El Ouaamari A, Dirice E, Gedeon N, Hu J, Zhou JY, Shirakawa J, et al. SerpinB1 promotes pancreatic \u03b2 cell proliferation. Cell Metab. 2016;23(1):194\u2013205.","journal-title":"Cell Metab"},{"key":"2454_CR9","doi-asserted-by":"publisher","first-page":"542","DOI":"10.1016\/j.freeradbiomed.2017.04.011","volume":"108","author":"W Yao","year":"2017","unstructured":"Yao W, Li H, Luo G, Li X, Chen C, Yuan D, et al. SERPINB1 ameliorates acute lung injury in liver transplantation through ERK1\/2-mediated STAT3-dependent HO-1 induction. Free Radical Biol Med. 2017;108:542\u201353.","journal-title":"Free Radical Biol Med"},{"issue":"1","key":"2454_CR10","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1002\/fsn3.1859","volume":"9","author":"X Liang","year":"2020","unstructured":"Liang X, Su Y, Huo Y. Forkhead box protein O1 (FoxO1)\/SERPINB1 ameliorates ROS production in diabetic nephropathy. Food Sci Nutr. 2020;9(1):44\u201351.","journal-title":"Food Sci Nutr"},{"issue":"2","key":"2454_CR11","doi-asserted-by":"publisher","first-page":"111","DOI":"10.2174\/1871529X11313020004","volume":"13","author":"K Johan","year":"2013","unstructured":"Johan K, Paul HAQ, Ilze B. Anti-apoptotic serpins as therapeutics in cardiovascular diseases. Cardiovasc Hematol Disord Drug Targets. 2013;13(2):111\u201322.","journal-title":"Cardiovasc Hematol Disord Drug Targets"},{"issue":"4","key":"2454_CR12","doi-asserted-by":"publisher","first-page":"1155","DOI":"10.2353\/ajpath.2006.060058","volume":"169","author":"I Petrache","year":"2006","unstructured":"Petrache I, Fijalkowska I, Medler TR, Skirball J, Cruz P, Zhen L, et al. alpha-1 antitrypsin inhibits caspase-3 activity, preventing lung endothelial cell apoptosis. Am J Pathol. 2006;169(4):1155\u201366.","journal-title":"Am J Pathol"},{"key":"2454_CR13","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1016\/j.yjmcc.2011.05.003","volume":"51","author":"S Toldo","year":"2011","unstructured":"Toldo S, Seropian I, Mezzaroma E, Van Tassell B, Salloum F, Lewis E, et al. Alpha-1 antitrypsin inhibits caspase-1 and protects from acute myocardial ischemia\u2013reperfusion injury. J Mol Cell Cardiol. 2011;51:244\u201351.","journal-title":"J Mol Cell Cardiol"},{"issue":"8","key":"2454_CR14","doi-asserted-by":"publisher","first-page":"1486","DOI":"10.1038\/sj.cdd.4402152","volume":"14","author":"JA Kummer","year":"2007","unstructured":"Kummer JA, Micheau O, Schneider P, Bovenschen N, Broekhuizen R, Quadir R, et al. Ectopic expression of the serine protease inhibitor PI9 modulates death receptor-mediated apoptosis. Cell Death Differ. 2007;14(8):1486\u201396.","journal-title":"Cell Death Differ"},{"issue":"1","key":"2454_CR15","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1016\/j.carpath.2010.12.002","volume":"21","author":"A Hendel","year":"2012","unstructured":"Hendel A, Cooper D, Abraham T, Zhao H, Allard MF, Granville DJ. Proteinase inhibitor 9 is reduced in human atherosclerotic lesion development. Cardiovasc Pathol. 2012;21(1):28\u201338.","journal-title":"Cardiovasc Pathol"},{"key":"2454_CR16","first-page":"1742","volume":"15","author":"D Na","year":"2017","unstructured":"Na D, Aijie H, Bo L, Zhilin M, Long Y. Gambogic acid exerts cardioprotective effects in a rat model of acute myocardial infarction through inhibition of inflammation, iNOS and NF-\u03baB\/p38 pathway. Exp Therapeutic Med. 2017;15:1742\u20138.","journal-title":"Exp Therapeutic Med"},{"key":"2454_CR17","doi-asserted-by":"publisher","first-page":"616813","DOI":"10.3389\/fphar.2020.616813","volume":"11","author":"C-Y Liu","year":"2021","unstructured":"Liu C-Y, Zhou Y, Chen T, Lei J-C, Jiang X-J. AMPK\/SIRT1 pathway is involved in arctigenin-mediated protective effects against myocardial ischemia-reperfusion injury. Front Pharmacol. 2021;11:616813\u2013616813.","journal-title":"Front Pharmacol"},{"key":"2454_CR18","doi-asserted-by":"publisher","first-page":"6989","DOI":"10.12659\/MSM.910930","volume":"24","author":"J Hua","year":"2018","unstructured":"Hua J, Liu Z, Liu Z, An D, Lai W, Zhan Q, et al. Metformin increases cardiac rupture after myocardial infarction via the AMPK-MTOR\/PGC-1\u03b1 signaling pathway in rats with acute myocardial infarction. Med Sci Monit. 2018;24:6989\u20137000.","journal-title":"Med Sci Monit"},{"key":"2454_CR19","doi-asserted-by":"publisher","first-page":"152764","DOI":"10.1016\/j.phymed.2018.11.024","volume":"58","author":"L Wang","year":"2019","unstructured":"Wang L, Yuan D, Zheng J, Wu X, Wang J, Liu X, et al. Chikusetsu saponin IVa attenuates isoprenaline-induced myocardial fibrosis in mice through activation autophagy mediated by AMPK\/mTOR\/ULK1 signaling. Phytomedicine. 2019;58:152764.","journal-title":"Phytomedicine"},{"issue":"17","key":"2454_CR20","doi-asserted-by":"crossref","first-page":"3126","DOI":"10.1111\/bph.14706","volume":"176","author":"J Yan","year":"2019","unstructured":"Yan J, Yan J-Y, Wang Y-X, Ling Y-N, Song X-D, Wang S-Y, et al. Spermidine-enhanced autophagic flux improves cardiac dysfunction following myocardial infarction by targeting the AMPK\/mTOR signalling pathway. Br J Pharmacol. 2019;176(17):3126\u201342.","journal-title":"Br J Pharmacol"},{"issue":"4","key":"2454_CR21","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1016\/j.gheart.2012.08.001","volume":"7","author":"K Thygesen","year":"2012","unstructured":"Thygesen K, Alpert JS, Jaffe AS, Simoons ML, Chaitman BR, White HD, et al. Third universal definition of myocardial infarction. Glob Heart. 2012;7(4):275\u201395.","journal-title":"Glob Heart"},{"issue":"1","key":"2454_CR22","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1189\/jlb.0810461","volume":"90","author":"C Benarafa","year":"2011","unstructured":"Benarafa C, LeCuyer TE, Baumann M, Stolley JM, Cremona TP, Remold-O\u2019Donnell E. SerpinB1 protects the mature neutrophil reserve in the bone marrow. J Leukoc Biol. 2011;90(1):21\u20139.","journal-title":"J Leukoc Biol"},{"key":"2454_CR23","doi-asserted-by":"publisher","first-page":"1055","DOI":"10.1016\/j.jsbmb.2019.105537","volume":"198","author":"S Xu","year":"2020","unstructured":"Xu S, Qin D, Yang H, He C, Liu W, Tian N, et al. SerpinB1 promotes the proliferation of porcine pancreatic stem cells through the STAT3 signaling pathway. J Steroid Biochem Mol Biol. 2020;198:1055.","journal-title":"J Steroid Biochem Mol Biol"},{"issue":"2","key":"2454_CR24","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1016\/j.yjmcc.2011.05.003","volume":"51","author":"S Toldo","year":"2011","unstructured":"Toldo S, Seropian IM, Mezzaroma E, Van Tassell BW, Salloum FN, Lewis EC, et al. Alpha-1 antitrypsin inhibits caspase-1 and protects from acute myocardial ischemia\u2013reperfusion injury. J Mol Cell Cardiol. 2011;51(2):244\u201351.","journal-title":"J Mol Cell Cardiol"},{"key":"2454_CR25","first-page":"1245","volume":"48","author":"S-M Jeon","year":"2016","unstructured":"Jeon S-M. Regulation and function of AMPK in physiology and diseases. Exp Mol Med. 2016;48:1245.","journal-title":"Exp Mol Med"},{"key":"2454_CR26","doi-asserted-by":"crossref","unstructured":"Tang M, Lusco M, Abate M, Levine J. Preconditioning mice with activators of AMPK ameliorates ischemic acute kidney injury in vivo. Am J Physiol Renal Physiol. 2016;311:ajprenal.00541.02015.","DOI":"10.1152\/ajprenal.00541.2015"},{"key":"2454_CR27","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1016\/j.bbrc.2017.08.034","volume":"492","author":"J Zhang","year":"2017","unstructured":"Zhang J, Zhao P, Quan N, Wang L, Chen X, Cates C, et al. The endotoxemia cardiac dysfunction is attenuated by AMPK\/mTOR signaling pathway regulating autophagy. Biochem Biophys Res Commun. 2017;492:520\u20137.","journal-title":"Biochem Biophys Res Commun"},{"key":"2454_CR28","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1159\/000480416","volume":"43","author":"J Hu","year":"2017","unstructured":"Hu J, Cui W, Ding W, Gu Y, Wang Z, Fan W. Globular adiponectin attenuated H2O2-induced apoptosis in rat chondrocytes by inducing autophagy through the AMPK\/ mTOR pathway. Cell Physiol Biochem Int J Exp Cell Physiol Biochem Pharmacol. 2017;43:367\u201382.","journal-title":"Cell Physiol Biochem Int J Exp Cell Physiol Biochem Pharmacol"},{"key":"2454_CR29","doi-asserted-by":"publisher","first-page":"1201","DOI":"10.1016\/j.biopha.2018.09.140","volume":"108","author":"H Chen","year":"2018","unstructured":"Chen H, Ji Y, Yan X, Su G, Chen L, Xiao J. Berberine attenuates apoptosis in rat retinal M\u00fcller cells stimulated with high glucose via enhancing autophagy and the AMPK\/mTOR signaling. Biomed Pharmacother. 2018;108:1201\u20137.","journal-title":"Biomed Pharmacother"},{"key":"2454_CR30","doi-asserted-by":"publisher","first-page":"273","DOI":"10.14336\/AD.2017.0517","volume":"9","author":"M Zhang","year":"2018","unstructured":"Zhang M, Deng Y-N, Zhang J-Y, Liu J, Li Y-B, Su H, et al. SIRT3 protects rotenone-induced injury in SH-SY5Y cells by promoting autophagy through the LKB1-AMPK-mTOR pathway. Aging Dis. 2018;9:273.","journal-title":"Aging Dis"},{"issue":"1\u20132","key":"2454_CR31","doi-asserted-by":"publisher","first-page":"394","DOI":"10.1016\/j.bbrc.2015.09.179","volume":"468","author":"Y Zhou","year":"2015","unstructured":"Zhou Y, He X, Chen Y, Huang Y, Wu L, He J. Exendin-4 attenuates cardiac hypertrophy via AMPK\/mTOR signaling pathway activation. Biochem Biophys Res Commun. 2015;468(1\u20132):394\u20139.","journal-title":"Biochem Biophys Res Commun"},{"issue":"1","key":"2454_CR32","first-page":"41","volume":"72","author":"L L\u00fc","year":"2017","unstructured":"L\u00fc L, Zhang D, Sun B, Hu Y, Yan M, Liu K, et al. Apocynum leaf extract inhibits the progress of atherosclerosis in rats via the AMPK\/mTOR pathway. Die Pharmazie Int J Pharmaceut Sci. 2017;72(1):41\u20138.","journal-title":"Die Pharmazie Int J Pharmaceut Sci"},{"issue":"2","key":"2454_CR33","first-page":"1582","volume":"18","author":"L Xu","year":"2018","unstructured":"Xu L, Jiang X, Wei F, Zhu H. Leonurine protects cardiac function following acute myocardial infarction through anti-apoptosis by the PI3K\/AKT\/GSK3\u03b2 signaling pathway. Mol Med Rep. 2018;18(2):1582\u201390.","journal-title":"Mol Med Rep"},{"issue":"3","key":"2454_CR34","first-page":"2095","volume":"19","author":"Z Yi","year":"2020","unstructured":"Yi Z, Ke J, Wang Y, Cai K. Fluvastatin protects myocardial cells in mice with acute myocardial infarction through inhibiting RhoA\/ROCK pathway. Exp Ther Med. 2020;19(3):2095\u2013102.","journal-title":"Exp Ther Med"}],"container-title":["BMC Cardiovascular Disorders"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12872-022-02454-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12872-022-02454-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12872-022-02454-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T21:32:31Z","timestamp":1700343151000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmccardiovascdisord.biomedcentral.com\/articles\/10.1186\/s12872-022-02454-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,15]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["2454"],"URL":"https:\/\/doi.org\/10.1186\/s12872-022-02454-7","relation":{},"ISSN":["1471-2261"],"issn-type":[{"value":"1471-2261","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,15]]},"assertion":[{"value":"5 August 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 January 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 March 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The experimental protocol of our study was performed in accordance with the Guide for the Care and Use of Laboratory Animals and approved by First people\u2019s Hospital of Jinan.The protocol of this research has been approved by the Ethics Committee of First people\u2019s Hospital of Jinan. All patients have signed written informed consent.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no conflicts of interest.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"107"}}