{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T16:59:10Z","timestamp":1781974750244,"version":"3.54.5"},"reference-count":33,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Infect Dis"],"published-print":{"date-parts":[[2017,12]]},"DOI":"10.1186\/s12879-017-2427-4","type":"journal-article","created":{"date-parts":[[2017,5,8]],"date-time":"2017-05-08T14:01:46Z","timestamp":1494252106000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Valine\/isoleucine variants drive selective pressure in the VP1 sequence of EV-A71 enteroviruses"],"prefix":"10.1186","volume":"17","author":[{"given":"Nghia Ngu","family":"Duy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le Thi Thanh","family":"Huong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Ravel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le Thi Song","family":"Huong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ankit","family":"Dwivedi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"October Michael","family":"Sessions","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2019An","family":"Hou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Chua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilhem","family":"Kister","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aneta","family":"Afelt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Moulia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duane J.","family":"Gubler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vu Dinh","family":"Thiem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Thi Hien","family":"Thanh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Devaux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tran Nhu","family":"Duong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Tran","family":"Hien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Cornillot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Gavotte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Frutos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,5,8]]},"reference":[{"key":"2427_CR1","doi-asserted-by":"publisher","first-page":"2104","DOI":"10.1093\/bioinformatics\/bti263","volume":"21","author":"F Abascal","year":"2005","unstructured":"Abascal F, Zardoya R, Posada D. ProtTest: selection of best-fit models of protein evolution. Bioinformatics. 2005;21:2104\u20135.","journal-title":"Bioinformatics"},{"key":"2427_CR2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0168-1702(99)00019-2","volume":"61","author":"S Abu Bakar","year":"1999","unstructured":"Abu Bakar S, Chee HY, Al-Kobaisi MF, Xiaoshan J, Bing CK, Kit LS. Identification of enterovirus 71 isolates from an outbreak of hand, foot and mouth disease (HFMD) with fatal cases of encephalomyelitis in Malaysia. Virus Res. 1999;61:1\u20139.","journal-title":"Virus Res"},{"key":"2427_CR3","doi-asserted-by":"crossref","first-page":"106","DOI":"10.47102\/annals-acadmedsg.V38N2p106","volume":"38","author":"LW Ang","year":"2009","unstructured":"Ang LW, Koh BK, Chan KP, Chua LT, James L, Goh KT. Epidemiology and control of hand, foot and mouth disease in Singapore. Ann Acad Med Singap. 2009;38:106\u201312.","journal-title":"Ann Acad Med Singap"},{"key":"2427_CR4","doi-asserted-by":"crossref","first-page":"9969","DOI":"10.1128\/JVI.73.12.9969-9975.1999","volume":"73","author":"BA Brown","year":"1999","unstructured":"Brown BA, Oberste MS, Alexander JP Jr, Kennett ML, Pallansch MA. Molecular epidemiology and evolution of enterovirus 71 strains isolated from 1970 to 1998. J Virol. 1999;73:9969\u201375.","journal-title":"J Virol"},{"key":"2427_CR5","doi-asserted-by":"publisher","first-page":"8592","DOI":"10.1128\/JVI.01370-16","volume":"90","author":"EA Caine","year":"2016","unstructured":"Caine EA, Moncla LH, Ronderos MD, Friedrich TC, Osorio JE. A single mutation in the VP1 of Enterovirus 71 is responsible for increased virulence and Neurotropism in adult interferon-deficient mice. J Virol. 2016;90:8592\u2013604.","journal-title":"J Virol"},{"key":"2427_CR6","unstructured":"Carter J, Saunders VA. Virology: principles and applications. Hoboken: John Wiley & Sons; 2007."},{"key":"2427_CR7","doi-asserted-by":"publisher","first-page":"e244","DOI":"10.1542\/peds.2006-3331","volume":"120","author":"KT Chen","year":"2007","unstructured":"Chen KT, Chang HL, Wang ST, Cheng YT, Yang JY. Epidemiologic features of hand-foot-mouth disease and herpangina caused by enterovirus 71 in Taiwan, 1998\u20132005. Pediatrics. 2007;120:e244\u201352.","journal-title":"Pediatrics"},{"key":"2427_CR8","doi-asserted-by":"publisher","unstructured":"Darriba D, Taboada GL, Doallo R, Posada D. jModelTest 2: more models, new heuristics and parallel computing. Nat Methods. 2012;9(8):772.","DOI":"10.1038\/nmeth.2109"},{"key":"2427_CR9","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1186\/1471-2105-5-113","volume":"5","author":"RC Edgar","year":"2004","unstructured":"Edgar RC. MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC bioinformatics. 2004;5:113.","journal-title":"BMC bioinformatics"},{"key":"2427_CR10","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1093\/molbev\/msp259","volume":"27","author":"M Gouy","year":"2010","unstructured":"Gouy M, Guindon S, Gascuel O. SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol Biol Evol. 2010;27:221\u20134.","journal-title":"Mol Biol Evol"},{"key":"2427_CR11","doi-asserted-by":"publisher","first-page":"936","DOI":"10.1056\/NEJM199909233411302","volume":"341","author":"CC Huang","year":"1999","unstructured":"Huang CC, Liu CC, Chang YC, Chen CY, Wang ST, Yeh TF. Neurologic complications in children with Enterovirus 71 infection. N Engl J Med. 1999;341:936\u201342.","journal-title":"N Engl J Med"},{"key":"2427_CR12","doi-asserted-by":"publisher","first-page":"11500","DOI":"10.1128\/JVI.02035-15","volume":"89","author":"SW Huang","year":"2005","unstructured":"Huang SW, Tai CH, Fonville JM, Lin CH., Wang SM, Liu CC, .Su IJ, Smith DJ, Wang JR. Mapping enterovirus A71 antigenic determinants from viral evolution. J Virol 2005; 89:11500-11506.","journal-title":"J Virol"},{"issue":"7","key":"2427_CR13","doi-asserted-by":"publisher","first-page":"e1005033","DOI":"10.1371\/journal.ppat.1005033","volume":"11","author":"C Kataoka","year":"2015","unstructured":"Kataoka C, Suzuki T, Kotani O, Iwata-Yoshikawa O, Nagata N, Ami Y, Wakita T, Nishimura Y, Shimizu H. The role of VP1 amino acid residue 145 of Enterovirus 71 in viral fitness and pathogenesis in a Cynomolgus monkey model. PLoS Pathog. 2015;11(7):e1005033.","journal-title":"PLoS Pathog"},{"key":"2427_CR14","doi-asserted-by":"publisher","first-page":"2002","DOI":"10.3201\/eid1812.120929","volume":"18","author":"TH Khanh","year":"2012","unstructured":"Khanh TH, Sabanathan S, Thanh TT, Thoa le PK, Thuong TC, Hang VT, Farrar J, Hien TT, Chau NV, van Doorn HR. Enterovirus 71-associated hand, foot, and mouth disease, southern Vietnam, 2011. Emerging Infect Dis. 2012;18:2002\u20135.","journal-title":"Emerging Infect Dis."},{"key":"2427_CR15","doi-asserted-by":"publisher","first-page":"582","DOI":"10.1002\/jmv.20579","volume":"78","author":"SK Lal","year":"2006","unstructured":"Lal SK, Kumar P, Yeo WM, Kar-Roy A, Chow VT. The VP1 protein of human enterovirus 71 self-associates via an interaction domain spanning amino acids 66\u2013297. J Med Virol. 2006;78:582\u201390.","journal-title":"J Med Virol"},{"issue":"10","key":"2427_CR16","doi-asserted-by":"publisher","first-page":"e26237","DOI":"10.1371\/journal.pone.0026237","volume":"6","author":"R Li","year":"2011","unstructured":"Li R, Zou Q, Chen L, Zhang H, Wang Y. Molecular analysis of virulent determinants of enterovirus 71. PLoS One. 2011;6(10):e26237.","journal-title":"PLoS One"},{"key":"2427_CR17","doi-asserted-by":"publisher","first-page":"1451","DOI":"10.1093\/bioinformatics\/btp187","volume":"25","author":"P Librado","year":"2009","unstructured":"Librado P, Rozas J. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics. 2009;25:1451\u20132.","journal-title":"Bioinformatics"},{"key":"2427_CR18","doi-asserted-by":"publisher","first-page":"7732","DOI":"10.1128\/JVI.75.16.7732-7738.2001","volume":"75","author":"P McMinn","year":"2001","unstructured":"McMinn P, Lindsay K, Perera D, Chan HM, Chan KP, Cardosa MJ. Phylogenetic analysis of enterovirus 71 strains isolated during linked epidemics in Malaysia, Singapore, and Western Australia. J Virol. 2001;75:7732\u20138.","journal-title":"J Virol"},{"key":"2427_CR19","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1186\/1471-2334-14-341","volume":"14","author":"NTB Nguyen","year":"2014","unstructured":"Nguyen NTB, Pham H, Hoang CQ, Nguyen TM, Nguyen LT, Phan HC, Phan LT, Vu LN, Minh NNT. Epidemiological and clinical characteristics of children who died from hand, foot and mouth disease in Vietnam, 2011. BMC Infect Dis. 2014;14:341.","journal-title":"BMC Infect Dis"},{"key":"2427_CR20","doi-asserted-by":"publisher","first-page":"2698","DOI":"10.1128\/JCM.00542-06","volume":"44","author":"WA Nix","year":"2006","unstructured":"Nix WA, Oberste MS, Pallansch MA. Sensitive, seminested PCR amplification of VP1 sequences for direct identification of all enterovirus serotypes from original clinical specimens. J Clin Microbiol. 2006;44:2698\u2013704.","journal-title":"J Clin Microbiol"},{"key":"2427_CR21","doi-asserted-by":"publisher","first-page":"1097","DOI":"10.1016\/S1474-4422(10)70209-X","volume":"9","author":"MH Ooi","year":"2010","unstructured":"Ooi MH, Wong SC, Lewthwaite P, Cardosa MJ, Solomon T. Clinical features, diagnosis, and management of enterovirus 71. The Lancet Neurology. 2010;9:1097\u2013105.","journal-title":"The Lancet Neurology"},{"key":"2427_CR22","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1186\/1471-2334-4-11","volume":"4","author":"D Perera","year":"2004","unstructured":"Perera D, Podin Y, Akin W, Tan CS, Cardosa MJ. Incorrect identification of recent Asian strains of Coxsackievirus A16 as human enterovirus 71: improved primers for the specific detection of human enterovirus 71 by RT PCR. BMC Infect Dis. 2004;4:11.","journal-title":"BMC Infect Dis"},{"key":"2427_CR23","doi-asserted-by":"publisher","first-page":"12572","DOI":"10.1128\/JVI.77.23.12572-12578.2003","volume":"77","author":"FW Peyerl","year":"2003","unstructured":"Peyerl FW, Barouch DH, Yeh WW, Bazick HS, Kunstman J, Kunstman KJ, Letvin NL. Simian-human immunodeficiency virus escape from cytotoxic T-lymphocyte recognition at a structurally constrained epitope. J Virol. 2003;77:12572\u20138.","journal-title":"J Virol"},{"key":"2427_CR24","doi-asserted-by":"publisher","first-page":"1003","DOI":"10.1007\/s00705-005-0684-9","volume":"151","author":"S Sanders","year":"2006","unstructured":"Sanders S, Herrero L, McPhie K, Chow S, Craig M, Dwyer D, Rawlinson W, McMinn PC. Molecular epidemiology of enterovirus 71 over two decades in an Australian urban community. Arch Virol. 2006;151:1003\u201313.","journal-title":"Arch Virol"},{"key":"2427_CR25","doi-asserted-by":"publisher","first-page":"648","DOI":"10.1093\/jac\/dki263","volume":"56","author":"M Schmidtke","year":"2005","unstructured":"Schmidtke M, Hammerschmidt E, Sch\u00fcler S, Zell R, Birch-Hirschfeld E, Makarov VA, Wutzler P. Susceptibility of coxsackievirus B3 laboratory strains and clinical isolates to the capsid function inhibitor pleconaril: antiviral studies with virus chimeras demonstrate the crucial role of amino acid 1092 in treatment. J Antimicrob Chemother. 2005;56:648\u201356.","journal-title":"J Antimicrob Chemother"},{"key":"2427_CR26","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1128\/JVI.64.2.467-475.1990","volume":"64","author":"PF Szurek","year":"1990","unstructured":"Szurek PF, Yuen PH, Ball JK, Wong PK. A Val-25-to-Ile substitution in the envelope precursor polyprotein, gPr80env, is responsible for the temperature sensitivity, inefficient processing of gPr80env, and neurovirulence of ts1, a mutant of Moloney murine leukemia virus TB. J Virol. 1990;64:467\u201375.","journal-title":"J Virol"},{"key":"2427_CR27","doi-asserted-by":"publisher","first-page":"3339","DOI":"10.1128\/JVI.01019-09","volume":"84","author":"KK Tee","year":"2010","unstructured":"Tee KK, Lam TTY, Chan YF, Bible JM, Kamarulzaman A, Tong C, Takebe Y, Pybus OG. Evolutionary genetics of human enterovirus 71: origin, population dynamics, natural selection, and seasonal periodicity of the VP1 gene. J Virol. 2010;84:3339\u201350.","journal-title":"J Virol"},{"key":"2427_CR28","doi-asserted-by":"publisher","first-page":"1733","DOI":"10.3201\/eid1311.070632","volume":"13","author":"PV Tu","year":"2007","unstructured":"Tu PV, Thao NTT, Perera D, Huu TK, Tien NTK, Thuong TC, How OM, Cardosa MJ, McMinn PC. Epidemiologic and virologic investigation of hand, foot, and mouth disease, southern Vietnam, 2005. Emerging Infect Dis. 2007;13:1733\u201341.","journal-title":"Emerging Infect Dis"},{"key":"2427_CR29","unstructured":"WHO. A Guide to Clinical Management and Public Health Response for Hand, Foot and Mouth Disease (HFMD). WHO WPRO; 2011."},{"key":"2427_CR30","doi-asserted-by":"publisher","first-page":"1601","DOI":"10.1099\/vir.0.031237-0","volume":"92","author":"Y Yamaguchi","year":"2011","unstructured":"Yamaguchi Y, Nukui Y, Tajima S, Nerome R, Kato F, Watanabe H, Kurane I. An amino acid substitution (V3I) in the Japanese encephalitis virus NS4A protein increases its virulence in mice, but not its growth rate in vitro. J Gen Virol. 2011;92:1601\u20136.","journal-title":"J Gen Virol"},{"key":"2427_CR31","doi-asserted-by":"publisher","first-page":"11809","DOI":"10.1128\/JVI.00297-11","volume":"85","author":"SL Yang","year":"2011","unstructured":"Yang SL, Chou YT, Wu CN, Ho MS. Annexin II binds to capsid protein VP1 of enterovirus 71 and enhances viral infectivity. J Virol. 2011;85:11809\u201320.","journal-title":"J Virol"},{"key":"2427_CR32","doi-asserted-by":"publisher","first-page":"204","DOI":"10.1016\/j.virol.2013.02.026","volume":"440","author":"F Yu","year":"2013","unstructured":"Yu F, Ren X, Wang Y, Qi X, Song J, Gao Y, Wang X. A single amino acid V4I substitution in VP1 attenuates virulence of very virulent infectious bursal disease virus (vvIBDV) in SPF chickens and increases replication in CEF cells. Virology. 2013;440:204\u20139.","journal-title":"Virology"},{"key":"2427_CR33","doi-asserted-by":"publisher","first-page":"1122","DOI":"10.1017\/S0950268811001622","volume":"140","author":"M Zeng","year":"2012","unstructured":"Zeng M, Li YF, Wang XH, Lu GP, Shen HG, Yu H, Zhu QR. Epidemiology of hand, foot, and mouth disease in children in shanghai 2007\u20132010. Epidemiol Infect. 2012;140:1122\u201330.","journal-title":"Epidemiol Infect"}],"container-title":["BMC Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12879-017-2427-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T13:12:36Z","timestamp":1692796356000},"score":1,"resource":{"primary":{"URL":"http:\/\/bmcinfectdis.biomedcentral.com\/articles\/10.1186\/s12879-017-2427-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,8]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["2427"],"URL":"https:\/\/doi.org\/10.1186\/s12879-017-2427-4","relation":{},"ISSN":["1471-2334"],"issn-type":[{"value":"1471-2334","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,8]]},"article-number":"333"}}